Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AKN91_RS09275 Genome accession   NZ_CP012362
Coordinates   1929362..1931053 (+) Length   563 a.a.
NCBI ID   WP_053109899.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain D3318     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1924362..1936053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN91_RS09235 (AKN91_09235) rpsM 1924454..1924810 (+) 357 WP_053100995.1 30S ribosomal protein S13 -
  AKN91_RS09240 (AKN91_09240) rpsK 1924841..1925230 (+) 390 WP_053100996.1 30S ribosomal protein S11 -
  AKN91_RS09245 (AKN91_09245) rpsD 1925249..1925869 (+) 621 WP_053100997.1 30S ribosomal protein S4 -
  AKN91_RS09250 (AKN91_09250) rpoA 1925892..1926893 (+) 1002 WP_053100998.1 DNA-directed RNA polymerase subunit alpha -
  AKN91_RS09255 (AKN91_09255) rplQ 1926937..1927320 (+) 384 WP_053100999.1 50S ribosomal protein L17 -
  AKN91_RS09260 (AKN91_09260) - 1927399..1927815 (+) 417 WP_053103338.1 PACE efflux transporter -
  AKN91_RS09265 (AKN91_09265) elbB 1927858..1928511 (+) 654 WP_053105042.1 isoprenoid biosynthesis glyoxalase ElbB -
  AKN91_RS09270 (AKN91_09270) - 1928582..1929091 (-) 510 WP_053105043.1 pilin -
  AKN91_RS09275 (AKN91_09275) pilB 1929362..1931053 (+) 1692 WP_053109899.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AKN91_RS09280 (AKN91_09280) pilC 1931059..1932276 (+) 1218 WP_053105045.1 type II secretion system F family protein Machinery gene
  AKN91_RS09285 (AKN91_09285) pilD 1932280..1933179 (+) 900 WP_053105046.1 A24 family peptidase Machinery gene
  AKN91_RS09290 (AKN91_09290) - 1933387..1933668 (-) 282 WP_053109901.1 GIY-YIG nuclease family protein -
  AKN91_RS09295 (AKN91_09295) - 1933933..1934922 (-) 990 WP_053105048.1 hypothetical protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62440.48 Da        Isoelectric Point: 6.2895

>NTDB_id=153798 AKN91_RS09275 WP_053109899.1 1929362..1931053(+) (pilB) [Thiopseudomonas alkaliphila strain D3318]
MELSSGLIGLAGQIVKLGLADYKKVLNSVADARLAGQPIISWLVHNKIVTSREVMTLATSQFGVASFDLSCFKIKLLSKD
LLSERIVRKHRVLPLAKRGNLLYVALADPGDQLVLNEIKFTTGCAVELVVVEQDKLDLLIEQLYDSLLESFESLADEVLE
ESEYIEIQNELIIDAIDEAPIVKFVNSMLLRAIKIGASDLHFEPYENSYRVRFRTDGILHEVSKQPVGLRDRISTRLKVM
ASIDISERRKPQDGRIKLKVSSVKSINFRVNTLPTLWGEKIVLRILDSDSTKLGIDSLGYESAQKKLYLEALDLPQGMIL
VTGPTGSGKSVSLYTGINIINTVERNISTAEDPVEIYLEGINQVNVNFKQGMDFAQALRAFLRQDPDVIMIGEIRDLETA
EIAIKAAQTGHMVMSTLHTNSAADTLTRLRNMGVAAFNLAISINLIIAQRLARRLCNICKSVKKIPVDILLQEGFSLEQV
GTFKVYEAVGCDKCNAGYKGRIGIYEVVKITPTLQAIIMEGGNPIEINQQIRKEGFNDLRTSALEKVIQGVVSLEEVNRV
TKD

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=153798 AKN91_RS09275 WP_053109899.1 1929362..1931053(+) (pilB) [Thiopseudomonas alkaliphila strain D3318]
ATGGAGTTATCAAGCGGCTTAATAGGTCTGGCAGGACAAATTGTCAAATTAGGTTTGGCAGACTATAAAAAGGTTTTAAA
CTCTGTTGCAGATGCTAGGCTGGCGGGCCAGCCAATTATTTCTTGGCTAGTGCATAATAAAATTGTCACTTCTCGGGAAG
TGATGACATTAGCTACAAGCCAATTTGGAGTAGCTAGTTTTGATTTAAGCTGTTTTAAAATAAAATTACTGAGTAAAGAT
TTACTTAGTGAAAGAATTGTTCGTAAACACCGAGTATTACCTTTAGCTAAGCGAGGCAATCTGCTTTATGTTGCCTTGGC
AGACCCTGGAGATCAGCTTGTACTTAATGAAATAAAATTTACGACTGGCTGTGCTGTTGAGCTAGTCGTAGTAGAGCAAG
ATAAATTGGATCTGCTTATTGAGCAACTTTATGACAGCTTGCTTGAGAGTTTTGAAAGTCTGGCTGATGAAGTCTTAGAA
GAGTCTGAGTATATTGAAATACAAAATGAGCTAATAATAGATGCTATAGATGAGGCTCCTATAGTTAAATTTGTAAATAG
TATGTTATTGCGTGCTATTAAAATAGGTGCTTCAGATTTACATTTTGAACCCTATGAAAATAGTTATAGAGTTCGCTTTC
GTACTGATGGGATATTGCATGAAGTAAGCAAGCAGCCTGTAGGTTTACGTGATCGGATCTCTACACGGCTCAAGGTTATG
GCTAGCATTGATATTTCTGAGCGTCGTAAACCACAAGACGGAAGAATTAAACTAAAGGTATCATCGGTAAAGTCTATAAA
TTTTCGAGTTAATACTTTACCTACTTTATGGGGTGAAAAAATTGTGCTAAGGATTTTAGATTCTGATAGTACAAAACTAG
GAATTGACTCGTTAGGCTATGAGTCAGCACAAAAAAAGCTTTATTTAGAAGCTTTAGATTTACCCCAAGGTATGATTTTA
GTGACTGGGCCTACTGGCTCGGGTAAAAGTGTATCTTTATATACAGGAATCAATATTATCAACACTGTAGAACGTAACAT
TTCTACTGCAGAAGATCCTGTGGAAATCTATTTGGAAGGGATTAATCAAGTTAATGTTAACTTCAAGCAAGGAATGGATT
TTGCACAAGCTTTGCGTGCTTTTTTACGACAGGATCCTGATGTAATAATGATTGGAGAAATTCGTGACTTAGAAACTGCA
GAAATTGCAATTAAGGCGGCACAAACTGGACACATGGTAATGTCAACTTTACATACAAATAGTGCTGCAGACACTCTTAC
ACGTCTACGTAATATGGGAGTAGCAGCCTTTAATTTAGCTATTTCGATTAATTTAATCATTGCCCAACGGTTAGCTAGAC
GTTTATGTAATATATGTAAGTCTGTTAAGAAAATACCAGTTGATATTTTATTGCAGGAGGGTTTTAGCTTAGAGCAAGTA
GGCACTTTTAAGGTATATGAGGCTGTTGGCTGTGATAAGTGTAATGCGGGATATAAAGGGCGAATTGGAATTTATGAAGT
GGTGAAAATTACACCTACATTACAAGCTATTATTATGGAAGGGGGGAATCCTATTGAAATTAACCAACAAATACGTAAAG
AAGGTTTTAATGATCTACGCACTTCAGCGTTAGAGAAGGTAATTCAGGGAGTTGTTAGTTTAGAAGAGGTGAATCGAGTA
ACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.617

96.448

0.536
  pilB Acinetobacter baylyi ADP1

54.025

99.29

0.536
  pilB Legionella pneumophila strain ERS1305867

52.533

94.671

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

47.292

98.401

0.465
  pilF Neisseria gonorrhoeae MS11

48.785

95.027

0.464
  pilB Vibrio campbellii strain DS40M4

46.085

99.822

0.46
  pilB Vibrio cholerae strain A1552

47.932

94.494

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.273

97.691

0.364
  pilB/pilB1 Synechocystis sp. PCC 6803

39.038

92.362

0.361

Multiple sequence alignment