Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACZ75_RS13855 Genome accession   NZ_CP012201
Coordinates   3418445..3420175 (+) Length   576 a.a.
NCBI ID   WP_050409289.1    Uniprot ID   A0A0K1JZ56
Organism   Massilia sp. NR 4-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3413445..3425175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACZ75_RS13830 (ACZ75_13875) uraH 3414395..3414745 (+) 351 WP_050409284.1 hydroxyisourate hydrolase -
  ACZ75_RS13835 (ACZ75_13880) - 3414789..3415289 (-) 501 WP_050409285.1 DUF4214 domain-containing protein -
  ACZ75_RS13840 (ACZ75_13885) - 3415453..3415950 (-) 498 WP_050409286.1 NADH-quinone oxidoreductase subunit B family protein -
  ACZ75_RS13845 - 3416038..3416871 (-) 834 WP_050409287.1 AraC family transcriptional regulator -
  ACZ75_RS13850 (ACZ75_13895) - 3417061..3418350 (+) 1290 WP_050409288.1 HlyC/CorC family transporter -
  ACZ75_RS13855 (ACZ75_13900) pilB 3418445..3420175 (+) 1731 WP_050409289.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACZ75_RS13860 (ACZ75_13905) pilC 3420232..3421449 (+) 1218 WP_050409290.1 type II secretion system F family protein Machinery gene
  ACZ75_RS13865 (ACZ75_13910) pilD 3421477..3422346 (+) 870 WP_050409291.1 A24 family peptidase Machinery gene
  ACZ75_RS13870 (ACZ75_13915) coaE 3422377..3423027 (+) 651 WP_050409292.1 dephospho-CoA kinase -
  ACZ75_RS13875 (ACZ75_13920) zapD 3423092..3423847 (+) 756 WP_050409293.1 cell division protein ZapD -
  ACZ75_RS13880 (ACZ75_13925) yacG 3423860..3424045 (+) 186 WP_050409294.1 DNA gyrase inhibitor YacG -
  ACZ75_RS13885 (ACZ75_13930) - 3424151..3424555 (-) 405 WP_050409295.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62182.85 Da        Isoelectric Point: 5.1015

>NTDB_id=152165 ACZ75_RS13855 WP_050409289.1 3418445..3420175(+) (pilB) [Massilia sp. NR 4-1]
MAAVQPSAVPGAPMPGLGRALIQAGRLSAPQAEALQKKSLNDKLAFIDALLGSGMMDARELAAFCSATFGYPLMDLQALN
PDALPPKLIEPRLMHGQRVLALARRGNKIAVAISDPTNTQALDQIKFQTESSVEPVIVPHDALLRLLTELGKDSEQAMNE
LAGEEGEIQFAEEEEAAAAAPDAAANEVEDAPIVRFLNKMLMDAVGMGASDLHFEPFEKFYRIRFRVDGVLIEHAQPPVS
IKDKLVSRIKVLAKLDISEKRVPQDGRMRLIVSPTKTIDLRISTLPTLFGEKTVMRILDATQAQMGIDSLGYEPDQRQLL
LDAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDI
IMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLGRRLCSCKQPVEIADEL
LLRAGYQQEELDGSWKPYGPVGCERCNGTGYKGRVGIYEIMPITPAIESLILAHGNAMQIAAQAQADGVKSLRQSGLVKV
KAGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=152165 ACZ75_RS13855 WP_050409289.1 3418445..3420175(+) (pilB) [Massilia sp. NR 4-1]
ATGGCAGCAGTCCAACCCAGTGCGGTCCCTGGCGCCCCCATGCCGGGCCTGGGACGGGCTTTGATCCAGGCCGGCCGGCT
CAGCGCGCCGCAGGCCGAGGCGCTGCAAAAAAAATCCCTCAACGATAAACTGGCGTTCATCGATGCGCTGCTGGGCAGCG
GCATGATGGACGCGCGCGAACTGGCCGCCTTCTGCTCCGCCACCTTCGGCTATCCGCTGATGGACTTGCAGGCGCTGAAC
CCCGACGCCCTGCCGCCCAAGCTGATCGAGCCGCGCCTGATGCACGGCCAGCGCGTGCTGGCCCTGGCGCGGCGCGGCAA
CAAGATCGCCGTCGCCATCTCCGATCCCACCAATACCCAGGCCCTGGACCAGATCAAGTTCCAGACCGAGTCGTCGGTGG
AACCGGTGATCGTGCCGCACGACGCCTTGCTGCGCCTGCTGACGGAGCTGGGCAAGGACAGCGAACAGGCGATGAACGAG
CTGGCCGGCGAGGAAGGCGAGATCCAGTTCGCCGAGGAGGAGGAAGCGGCGGCCGCCGCGCCCGACGCCGCCGCCAACGA
GGTCGAGGACGCGCCCATCGTGCGCTTCCTGAACAAGATGCTGATGGATGCGGTGGGCATGGGCGCCTCCGACCTGCATT
TCGAACCGTTTGAAAAGTTTTACCGCATCCGCTTCCGCGTCGACGGCGTGCTGATCGAGCATGCGCAGCCGCCGGTGTCG
ATCAAGGACAAGCTGGTGTCGCGCATCAAGGTGCTGGCCAAGCTCGATATCTCGGAAAAGCGCGTGCCGCAGGATGGCCG
CATGCGCCTGATCGTTTCGCCCACCAAGACCATCGACCTGCGCATCTCGACCCTGCCCACCCTGTTCGGCGAAAAGACCG
TGATGCGTATCCTGGACGCCACGCAGGCGCAGATGGGCATCGACTCCCTCGGCTACGAGCCGGACCAGCGGCAACTGCTG
CTGGATGCCATCCAGCGTCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACAC
CTGCCTGAACATCCTGAACAAACCGGGCATCAATATCTCGACGGCGGAAGACCCGGCCGAGATCAATCTGCCCGGCGTCA
ACCAGGTCAACGTCAACGACAAGGCGGGCCTGACCTTCCCGGTGGCGCTGAAATCCTTCCTGCGGCAAGACCCGGACATC
ATCATGGTGGGCGAGATCCGCGACCTGGAGACGGCCGATATCGCCATCAAGGCGGCACAGACCGGGCATATGGTGTTCTC
CACCCTGCACACCAACGACGCGCCCTCGACCCTGACGCGGCTGATGAATATGGGCGTGGCGCCGTTCAATATCGCCTCCT
CCGTGATCCTGATCACGGCCCAGCGCCTGGGCCGCCGCCTGTGCAGCTGCAAGCAGCCGGTGGAGATCGCGGACGAGCTG
CTGCTGCGCGCCGGCTACCAGCAGGAAGAGCTGGATGGCAGCTGGAAGCCGTATGGCCCGGTGGGCTGCGAACGCTGCAA
CGGCACCGGCTACAAGGGCCGGGTCGGCATTTACGAGATCATGCCGATCACGCCCGCCATCGAGTCGCTGATCCTGGCGC
ATGGCAATGCGATGCAGATCGCCGCCCAGGCACAGGCCGACGGCGTGAAGTCGCTGCGCCAATCGGGACTGGTCAAGGTC
AAGGCCGGCCTGACCAGCCTGGAGGAAGTGCTGGGCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1JZ56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.055

100

0.528
  pilF Neisseria gonorrhoeae MS11

52.817

98.611

0.521
  pilB Acinetobacter baylyi ADP1

52.753

97.743

0.516
  pilB Legionella pneumophila strain ERS1305867

46.986

97.917

0.46
  pilB Vibrio cholerae strain A1552

47.302

96.528

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

46.631

97.917

0.457
  pilB Vibrio campbellii strain DS40M4

45.406

98.264

0.446
  pilF Thermus thermophilus HB27

39.965

98.611

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.236

100

0.392

Multiple sequence alignment