Detailed information NTG0152167

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACZ75_RS13865
• Genome accession: NZ_CP012201
• Coordinates: 3421477..3422346 (+)
• Length: 289 a.a.
• NCBI ID: WP_050409291.1
• Uniprot ID: A0A0K1JZ69
• Organism: Massilia sp. NR 4-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3416477..3427346

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACZ75_RS13850 (ACZ75_13895)	-	3417061..3418350 (+)	1290	WP_050409288.1	HlyC/CorC family transporter	-
ACZ75_RS13855 (ACZ75_13900)	pilB	3418445..3420175 (+)	1731	WP_050409289.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACZ75_RS13860 (ACZ75_13905)	pilC	3420232..3421449 (+)	1218	WP_050409290.1	type II secretion system F family protein	Machinery gene
ACZ75_RS13865 (ACZ75_13910)	pilD	3421477..3422346 (+)	870	WP_050409291.1	A24 family peptidase	Machinery gene
ACZ75_RS13870 (ACZ75_13915)	coaE	3422377..3423027 (+)	651	WP_050409292.1	dephospho-CoA kinase	-
ACZ75_RS13875 (ACZ75_13920)	zapD	3423092..3423847 (+)	756	WP_050409293.1	cell division protein ZapD	-
ACZ75_RS13880 (ACZ75_13925)	yacG	3423860..3424045 (+)	186	WP_050409294.1	DNA gyrase inhibitor YacG	-
ACZ75_RS13885 (ACZ75_13930)	-	3424151..3424555 (-)	405	WP_050409295.1	NUDIX domain-containing protein	-
ACZ75_RS13890 (ACZ75_13935)	-	3424552..3425433 (-)	882	WP_050409296.1	ATP-binding protein	-
ACZ75_RS13895 (ACZ75_13940)	argJ	3425437..3426675 (-)	1239	WP_050409297.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31016.78 Da
• Isoelectric Point: 8.3290

>NTDB_id=152167 ACZ75_RS13865 WP_050409291.1 3421477..3422346(+) (pilD) [Massilia sp. NR 4-1]
MPEYLMLFSPPGTPGAAIIAGIFGLMIGSFLNVVIYRLPKMMQRESDNYVAAESGKQLPHTDRFNLMVPRSACPHCGHQV
TALENVPVFSWLALRGKCSGCKNPISARYPLIEALTALLSAGLVWQFGSGYMGLATLVFAWMLIAMTFIDADTQLLPDDL
TYPLLWAGLLVNLNGTFVPLRDAVIGAAAGYLALWSIFWLFKLATGKEGMGYGDFKLLAALGAWLGWAMLPSIILLSSVV
GAVVGICLIVFARHGRNNPIPFGPYLAAAGMIALIFGAPISRFTSSLAG

Nucleotide

• Length: 870 bp

>NTDB_id=152167 ACZ75_RS13865 WP_050409291.1 3421477..3422346(+) (pilD) [Massilia sp. NR 4-1]
ATGCCTGAATACCTCATGCTGTTTTCGCCGCCCGGCACGCCGGGCGCCGCCATCATCGCCGGCATTTTCGGCCTGATGAT
AGGCAGTTTCCTGAACGTGGTGATCTACCGCCTGCCGAAGATGATGCAGCGCGAGTCGGACAATTATGTGGCAGCCGAAT
CGGGCAAGCAGCTGCCGCATACCGACCGCTTCAACCTGATGGTGCCGCGTTCGGCCTGCCCGCACTGCGGCCACCAGGTG
ACGGCACTGGAAAACGTCCCCGTGTTCAGCTGGCTGGCCCTGCGCGGCAAATGCAGCGGCTGCAAGAACCCGATCTCGGC
GCGCTATCCGCTGATCGAGGCCTTGACCGCCCTGCTGTCGGCGGGCCTGGTGTGGCAATTCGGCAGCGGCTATATGGGCC
TGGCCACCCTGGTCTTCGCCTGGATGCTGATCGCCATGACCTTCATCGACGCCGATACCCAGCTGCTGCCGGACGACCTG
ACCTACCCGCTGCTGTGGGCCGGCCTGCTGGTGAACCTGAACGGCACCTTCGTGCCGCTGCGCGATGCCGTGATCGGCGC
CGCCGCCGGCTATCTGGCGCTATGGAGCATCTTCTGGCTCTTCAAGCTGGCGACGGGCAAGGAGGGAATGGGGTATGGTG
ACTTCAAGCTGCTGGCGGCGCTGGGCGCCTGGCTGGGCTGGGCCATGCTGCCATCGATCATCCTGCTGTCCTCGGTGGTG
GGCGCGGTGGTCGGCATCTGCCTGATCGTCTTTGCGCGGCACGGCCGCAACAACCCGATTCCCTTTGGTCCTTATCTGGC
GGCGGCCGGCATGATCGCGCTGATCTTCGGCGCACCGATCAGCCGTTTCACCAGCAGCCTGGCGGGCTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

• Type IV leader peptidase family (Peptidase_A24) (137-246)

3D structure

• AlphaFold DB: A0A0K1JZ69

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.895	92.042	0.533
pilD	Vibrio campbellii strain DS40M4	55.797	95.502	0.533
pilD	Neisseria gonorrhoeae MS11	50	91.349	0.457
pilD	Acinetobacter baumannii D1279779	49.438	92.388	0.457
pilD	Acinetobacter nosocomialis M2	48.315	92.388	0.446