Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ADJ79_RS09925 Genome accession   NZ_CP012073
Coordinates   2264339..2266063 (-) Length   574 a.a.
NCBI ID   WP_050715822.1    Uniprot ID   -
Organism   Ottowia sp. oral taxon 894 strain W10237     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2259339..2271063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ADJ79_RS13305 - 2260517..2260717 (-) 201 WP_082203248.1 DNA gyrase inhibitor YacG -
  ADJ79_RS09905 (ADJ79_09970) zapD 2260714..2261469 (-) 756 WP_050715818.1 cell division protein ZapD -
  ADJ79_RS09910 (ADJ79_09975) coaE 2261503..2262108 (-) 606 WP_050715819.1 dephospho-CoA kinase -
  ADJ79_RS09915 (ADJ79_09980) - 2262130..2263071 (-) 942 WP_050715820.1 A24 family peptidase -
  ADJ79_RS09920 (ADJ79_09985) pilC 2263075..2264304 (-) 1230 WP_050715821.1 type II secretion system F family protein Machinery gene
  ADJ79_RS09925 (ADJ79_09990) pilB 2264339..2266063 (-) 1725 WP_050715822.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ADJ79_RS09935 (ADJ79_10000) - 2266329..2267309 (-) 981 WP_050715823.1 polyprenyl synthetase family protein -
  ADJ79_RS09940 (ADJ79_10005) - 2267363..2268493 (-) 1131 WP_050715824.1 WG repeat-containing protein -
  ADJ79_RS09945 (ADJ79_10010) - 2268505..2270448 (-) 1944 WP_050715825.1 RecQ family ATP-dependent DNA helicase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62634.65 Da        Isoelectric Point: 4.7807

>NTDB_id=151082 ADJ79_RS09925 WP_050715822.1 2264339..2266063(-) (pilB) [Ottowia sp. oral taxon 894 strain W10237]
MATVESPAQAAPLPGLAHTLVMTGKLGQQAAEDLYKKAQQSNKNFIAELTGSGAVSAFDLAHTISAAFAAPLIDAAAIDP
NRLPQDLIDRKLIVKYRILPLSKRGNRLIIATADPTDNEAAEKVKFATQMGVEWVIAEYDKLSHLIETATRGTGDGMDGI
SADDFEFGEDAASPNEAEEDAEQDDSEDTPIVRFLQKMLVDAVSMRASDLHFEPYEFMYRVRFRIDGELREIASPPPGIK
EKLASRIKVISRLDISEKRVPQDGRMKLKLGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPIEKERLMT
AIERPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPAEINLPGINQVNMNDKAGLTFAVALRAFLRQDPDIIM
VGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPKCKEPTDIPNEAL
LEAGFTEEDLDGSWTPYRPVGCDACNNGYKGRVGIYQVMPISEEMQRIILADGSALEISDQARSEGVRSLRESGLHKVKI
GVTSLEEVLAVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=151082 ADJ79_RS09925 WP_050715822.1 2264339..2266063(-) (pilB) [Ottowia sp. oral taxon 894 strain W10237]
ATGGCAACCGTTGAATCCCCCGCACAAGCAGCGCCTCTGCCCGGCTTGGCGCACACGCTGGTCATGACTGGCAAGCTCGG
CCAGCAAGCCGCTGAAGACCTCTACAAGAAGGCGCAGCAGTCCAACAAAAATTTCATTGCGGAGCTAACTGGCTCCGGCG
CGGTTTCCGCCTTCGATCTGGCGCACACCATTTCCGCTGCATTCGCGGCGCCGCTCATTGATGCCGCGGCCATTGACCCC
AACCGCCTCCCGCAGGATCTGATCGATCGCAAGCTGATCGTCAAATACCGCATCCTGCCTCTAAGCAAGAGAGGCAACCG
GCTCATCATCGCAACAGCTGATCCCACGGATAACGAAGCTGCAGAAAAAGTCAAGTTCGCCACCCAGATGGGTGTGGAAT
GGGTCATTGCCGAATATGACAAACTTTCCCATCTGATCGAGACAGCCACTCGCGGCACAGGCGATGGAATGGACGGCATC
AGCGCCGACGACTTCGAATTTGGCGAAGATGCCGCCAGCCCCAATGAAGCCGAAGAAGATGCTGAACAAGATGACAGCGA
AGACACTCCTATCGTTCGCTTCCTGCAAAAAATGCTGGTAGACGCCGTCAGTATGCGCGCCTCTGACTTGCATTTTGAAC
CCTACGAATTCATGTACCGGGTGCGTTTTCGCATAGATGGCGAATTGCGCGAAATTGCCAGTCCTCCACCCGGCATCAAA
GAAAAACTGGCTTCTCGCATCAAGGTTATTTCGCGTCTTGATATTTCCGAAAAACGCGTGCCGCAAGATGGCCGCATGAA
GCTCAAACTGGGGCCTGATCGCGTGATTGATTTCCGCGTCAGCACCTTGCCCACCCTGTTTGGCGAAAAAATCGTGATCC
GTATTCTGGACCCGAGCAGCGCCAAGTTGGGAATTGATGCCCTCGGCTACGAACCAATTGAAAAAGAGCGTTTGATGACG
GCCATTGAGCGCCCCTATGGCATGATTCTCGTTACCGGCCCCACTGGCTCTGGCAAAACTGTTTCGCTCTATACTTGTCT
CAACTTGTTAAACAAGCCAGGGGTCAATATATCCACTGCAGAGGATCCGGCTGAAATCAACTTGCCTGGCATCAACCAGG
TCAATATGAATGACAAGGCTGGCCTGACTTTTGCTGTCGCCCTCAGGGCCTTTCTCCGTCAGGATCCGGATATCATCATG
GTAGGTGAAATTCGTGATCTGGAAACAGCTGACATTGCCATCAAGGCGGCCCAGACAGGGCATTTAGTATTATCCACACT
GCATACCAACGATGCTCCGACAACGTTAACGCGCATGCGAAATATGGGGATCGCCCCATTTAACATCGCATCCAGCGTCA
TTCTTATCACAGCCCAACGCTTGGCGCGGCGACTCTGCCCAAAATGCAAGGAGCCCACTGATATACCCAATGAAGCTCTC
TTAGAGGCCGGGTTTACAGAAGAAGATCTGGATGGAAGCTGGACACCCTACAGACCTGTCGGATGCGACGCATGCAACAA
CGGTTATAAAGGCCGGGTCGGCATCTATCAAGTCATGCCTATTTCAGAAGAGATGCAGCGCATTATTCTCGCTGATGGGA
GCGCCCTGGAGATATCAGACCAGGCTCGCTCCGAGGGAGTCCGTTCATTGCGTGAGTCTGGTCTGCACAAAGTAAAAATA
GGCGTCACTTCGCTTGAAGAAGTCTTGGCCGTTACTAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-148)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.923

95.645

0.554
  pilB Acinetobacter baylyi ADP1

55.16

97.909

0.54
  pilF Neisseria gonorrhoeae MS11

53.546

98.258

0.526
  pilB Legionella pneumophila strain ERS1305867

50.355

98.258

0.495
  pilB Vibrio campbellii strain DS40M4

47.979

99.129

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

47.594

97.735

0.465
  pilB Vibrio cholerae strain A1552

49.906

92.509

0.462
  pilF Thermus thermophilus HB27

39.791

99.826

0.397
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

92.334

0.392

Multiple sequence alignment