Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   REIFOR_RS13215 Genome accession   NZ_CP011797
Coordinates   2885780..2887471 (+) Length   563 a.a.
NCBI ID   WP_100258788.1    Uniprot ID   A0A2K8KVI0
Organism   Reinekea forsetii strain Hel1_31_D35     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2880780..2892471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  REIFOR_RS13185 (REIFOR_02650) minE 2880936..2881187 (+) 252 WP_100258006.1 cell division topological specificity factor MinE -
  REIFOR_RS13190 (REIFOR_02651) trxB 2881299..2882243 (-) 945 WP_100258007.1 thioredoxin-disulfide reductase -
  REIFOR_RS13195 (REIFOR_02652) ampE 2882385..2883188 (-) 804 WP_100258008.1 regulatory signaling modulator protein AmpE -
  REIFOR_RS13200 (REIFOR_02654) nadC 2883396..2884253 (+) 858 WP_100258009.1 carboxylating nicotinate-nucleotide diphosphorylase -
  REIFOR_RS13205 (REIFOR_02655) - 2884259..2884747 (-) 489 WP_100258010.1 pilin -
  REIFOR_RS13210 (REIFOR_02656) - 2884886..2885449 (-) 564 WP_100258011.1 pilin -
  REIFOR_RS13215 (REIFOR_02657) pilB 2885780..2887471 (+) 1692 WP_100258788.1 type IV-A pilus assembly ATPase PilB Machinery gene
  REIFOR_RS13220 (REIFOR_02658) pilC 2887485..2888708 (+) 1224 WP_100258012.1 type II secretion system F family protein Machinery gene
  REIFOR_RS13225 (REIFOR_02659) pilD 2888708..2889580 (+) 873 WP_100258013.1 A24 family peptidase Machinery gene
  REIFOR_RS13230 (REIFOR_02660) coaE 2889577..2890173 (+) 597 WP_227003691.1 dephospho-CoA kinase -
  REIFOR_RS13235 (REIFOR_02661) - 2890185..2890394 (+) 210 WP_100258014.1 DNA gyrase inhibitor YacG -
  REIFOR_RS13240 (REIFOR_02662) argJ 2890457..2891680 (-) 1224 WP_100258015.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61193.28 Da        Isoelectric Point: 5.0595

>NTDB_id=148750 REIFOR_RS13215 WP_100258788.1 2885780..2887471(+) (pilB) [Reinekea forsetii strain Hel1_31_D35]
MALGGLARRLVVEGSISEEAAVSAANEASRAKKGLVPYLVENDLIGARKIAVIAADEFGTPFFDLDGFDIELFPKDLIAN
NLIIAHHAIPLVKRGTRLFVGVSDPTNLVAIDEIKFHTGLSVDAILVEEDKLRSFIERITDDASDAFDNLEDEGLDDLDV
VDTQTRAEDKAVGDGSDDAPVVRFINQMLLSAIKKGASDLHFEPYEKSYRVRFRIDGILQTAAKPPIALGPKMSARLKVM
SNLDISERRVPQDGRIKLKISKTKAIDFRVNTLPTLFGEKIVMRILDPSSAKMGIDALGYELVQKEMYLKALDKPQGMIL
VTGPTGSGKTVSLYTGINILNQDGINISTAEDPVEINLEGINQVNVNTKVGLDFSSALRSFLRQDPDIIMVGEIRDLETA
SIAIKAAQTGHMVMSTLHTNSAAETITRMLNMGVPSFNLATTINLIIAQRLARRLCSKCKEPADLSAEQLVKAGFSSELA
QTATIFKPKGCDSCTGGYKGRVGIYEVVRITPDVSRIIMDEGNAIQLNEQFKVEGFNSLRISGLVKAASGVTSLEEVNRI
TTD

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=148750 REIFOR_RS13215 WP_100258788.1 2885780..2887471(+) (pilB) [Reinekea forsetii strain Hel1_31_D35]
ATGGCATTAGGGGGCCTGGCACGGCGCCTGGTAGTAGAGGGGTCCATATCTGAAGAGGCCGCCGTAAGCGCGGCCAACGA
GGCCAGCAGAGCGAAAAAGGGCCTGGTGCCCTATCTGGTCGAAAACGACCTGATCGGTGCGCGCAAGATTGCGGTCATTG
CCGCCGATGAATTTGGCACGCCCTTTTTTGACCTGGACGGGTTCGATATTGAGCTGTTCCCAAAAGATCTGATCGCCAAC
AATCTCATTATCGCCCATCACGCCATTCCGCTGGTTAAGCGCGGCACCCGCCTGTTTGTCGGGGTCTCGGATCCAACCAA
TCTAGTGGCCATCGATGAAATCAAGTTCCACACCGGCCTAAGTGTCGATGCGATCTTGGTCGAAGAAGATAAGTTGCGCA
GCTTTATTGAGCGAATCACCGATGACGCCTCCGATGCGTTCGATAACTTAGAGGATGAGGGACTCGACGATCTTGATGTG
GTCGACACCCAAACTCGCGCGGAGGATAAGGCCGTTGGTGACGGCAGCGACGATGCCCCGGTGGTGCGCTTTATCAACCA
GATGCTGCTCAGTGCGATTAAAAAAGGCGCGTCGGACCTGCATTTTGAACCCTATGAAAAGTCTTATCGGGTCCGCTTCC
GCATTGATGGCATTTTACAAACCGCGGCCAAGCCACCCATTGCCCTAGGGCCAAAGATGTCGGCGCGCTTGAAGGTAATG
TCGAATCTGGACATTTCCGAGCGCCGTGTACCCCAGGACGGGCGCATCAAGTTAAAAATTTCCAAAACCAAGGCCATCGA
TTTCCGCGTCAACACCCTGCCGACGCTGTTTGGCGAAAAAATCGTTATGCGGATTCTCGATCCCTCGTCAGCCAAGATGG
GCATCGACGCATTGGGCTATGAACTGGTGCAAAAAGAAATGTATCTAAAAGCACTCGATAAACCCCAAGGCATGATTTTA
GTGACCGGCCCAACGGGTTCGGGTAAAACCGTCTCCCTCTATACCGGCATTAATATTCTTAACCAGGACGGTATTAATAT
CTCAACCGCCGAAGATCCGGTCGAGATCAATCTAGAGGGTATTAACCAGGTCAACGTCAACACCAAGGTCGGCCTGGACT
TCTCCTCGGCCCTGCGCTCTTTTTTGCGCCAGGACCCGGATATCATTATGGTCGGCGAAATCCGCGACCTGGAAACGGCG
TCCATCGCCATTAAGGCAGCCCAGACCGGTCACATGGTCATGTCGACACTGCACACCAATAGCGCGGCAGAAACCATTAC
CCGGATGCTCAATATGGGTGTGCCATCCTTTAACCTGGCCACCACCATCAATCTCATTATCGCCCAACGTTTGGCCCGCC
GGCTGTGCAGCAAGTGTAAGGAGCCGGCCGACTTAAGCGCAGAGCAGCTGGTAAAGGCCGGCTTTTCCAGTGAGTTGGCC
CAGACGGCGACAATCTTCAAACCCAAGGGTTGTGACAGTTGCACCGGCGGGTACAAGGGCCGTGTTGGTATTTATGAAGT
GGTTCGCATCACCCCAGACGTGTCCCGTATTATTATGGATGAGGGAAACGCGATCCAACTCAATGAACAGTTCAAGGTTG
AGGGCTTTAACAGCCTAAGGATCTCGGGTCTGGTGAAGGCGGCCTCGGGCGTCACCAGTCTGGAAGAAGTAAACAGAATT
ACAACCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8KVI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.646

100

0.599
  pilB Acinetobacter baumannii D1279779

59.503

100

0.595
  pilB Legionella pneumophila strain ERS1305867

51.499

100

0.519
  pilB Vibrio cholerae strain A1552

50.365

97.336

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.023

100

0.49
  pilF Neisseria gonorrhoeae MS11

47.957

100

0.48
  pilB Vibrio campbellii strain DS40M4

50.677

91.829

0.465

Multiple sequence alignment