Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   REIFOR_RS13225 Genome accession   NZ_CP011797
Coordinates   2888708..2889580 (+) Length   290 a.a.
NCBI ID   WP_100258013.1    Uniprot ID   A0A2K8KSU7
Organism   Reinekea forsetii strain Hel1_31_D35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2883708..2894580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  REIFOR_RS13205 (REIFOR_02655) - 2884259..2884747 (-) 489 WP_100258010.1 pilin -
  REIFOR_RS13210 (REIFOR_02656) - 2884886..2885449 (-) 564 WP_100258011.1 pilin -
  REIFOR_RS13215 (REIFOR_02657) pilB 2885780..2887471 (+) 1692 WP_100258788.1 type IV-A pilus assembly ATPase PilB Machinery gene
  REIFOR_RS13220 (REIFOR_02658) pilC 2887485..2888708 (+) 1224 WP_100258012.1 type II secretion system F family protein Machinery gene
  REIFOR_RS13225 (REIFOR_02659) pilD 2888708..2889580 (+) 873 WP_100258013.1 A24 family peptidase Machinery gene
  REIFOR_RS13230 (REIFOR_02660) coaE 2889577..2890173 (+) 597 WP_227003691.1 dephospho-CoA kinase -
  REIFOR_RS13235 (REIFOR_02661) - 2890185..2890394 (+) 210 WP_100258014.1 DNA gyrase inhibitor YacG -
  REIFOR_RS13240 (REIFOR_02662) argJ 2890457..2891680 (-) 1224 WP_100258015.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  REIFOR_RS13245 (REIFOR_02663) secA 2891726..2894440 (-) 2715 WP_100258016.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32098.19 Da        Isoelectric Point: 6.6800

>NTDB_id=148752 REIFOR_RS13225 WP_100258013.1 2888708..2889580(+) (pilD) [Reinekea forsetii strain Hel1_31_D35]
MIDYLSTNTSALIVTVFIVGLLIGSFLNVVIYRLPAMMHKAWAVEVAQFNSDVDLLDRLQREPTYNLVTPDSSCPQCGHK
IRAWENIPLISWLMLRARCSACHKPISARYPAVELITALLSALVAWQFGWSLTSLCLIPLVWVLVTLTLIDVDTQLLPDS
LTLPLLWAGLLLNSQEIIVSLNSAVIGAAAGYLSLWSIYWLFKLLTGKDGMGYGDFKLFAAFGAWFGWIALPLIILLSSL
VGAVVGIVMILSLGRDRQVPIPFGPYLCGAALVYIFWGQSIMNWYLGYLV

Nucleotide


Download         Length: 873 bp        

>NTDB_id=148752 REIFOR_RS13225 WP_100258013.1 2888708..2889580(+) (pilD) [Reinekea forsetii strain Hel1_31_D35]
ATGATTGACTATCTGTCCACAAACACCAGCGCCTTGATAGTAACCGTCTTTATTGTCGGTTTACTGATCGGCAGCTTTCT
CAATGTTGTCATCTACCGCCTACCGGCGATGATGCACAAGGCCTGGGCGGTGGAAGTGGCTCAGTTCAATAGTGACGTAG
ACCTACTCGATCGACTCCAACGGGAACCGACTTACAATCTGGTCACACCGGACTCCAGTTGCCCGCAGTGCGGCCACAAG
ATTCGGGCCTGGGAGAACATTCCGCTGATCAGCTGGCTGATGCTTCGAGCTCGATGTTCGGCCTGTCACAAGCCTATTTC
GGCCCGTTACCCAGCAGTGGAGCTCATCACGGCCTTACTCTCGGCGCTGGTTGCTTGGCAGTTTGGCTGGTCTCTAACCA
GTCTATGCCTTATTCCGCTGGTCTGGGTGTTGGTGACCCTGACCCTAATCGATGTCGATACCCAACTACTGCCCGACAGC
CTCACCCTGCCCCTGCTCTGGGCCGGTTTGCTGCTAAACAGCCAAGAGATCATAGTGAGCTTGAACAGTGCCGTTATCGG
TGCCGCCGCAGGCTATCTCAGCCTCTGGTCAATTTACTGGCTCTTTAAACTGCTTACCGGCAAGGACGGCATGGGCTATG
GTGACTTTAAATTGTTTGCCGCCTTCGGTGCCTGGTTTGGCTGGATTGCTCTACCACTGATAATATTATTGTCCTCCCTG
GTGGGCGCGGTGGTCGGTATCGTGATGATTCTCAGTCTGGGCCGCGATCGACAGGTGCCTATACCGTTCGGCCCTTACCT
CTGTGGCGCCGCCCTGGTTTATATCTTTTGGGGCCAGAGCATAATGAACTGGTATCTGGGGTATCTGGTTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8KSU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

51.389

99.31

0.51
  pilD Acinetobacter baumannii D1279779

51.042

99.31

0.507
  pilD Vibrio campbellii strain DS40M4

51.613

96.207

0.497
  pilD Neisseria gonorrhoeae MS11

50.362

95.172

0.479
  pilD Vibrio cholerae strain A1552

51.481

93.103

0.479

Multiple sequence alignment