Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PDR5_RS15890 Genome accession   NZ_CP011566
Coordinates   3565667..3566779 (-) Length   370 a.a.
NCBI ID   WP_064596992.1    Uniprot ID   -
Organism   Pseudomonas sp. DR 5-09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3560667..3571779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PDR5_RS15885 (PDR5_31960) - 3561584..3565447 (-) 3864 WP_156669339.1 hypothetical protein -
  PDR5_RS15890 (PDR5_31970) pilU 3565667..3566779 (-) 1113 WP_064596992.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PDR5_RS15895 (PDR5_31980) - 3566908..3569445 (+) 2538 WP_064596994.1 PAS domain-containing sensor histidine kinase -
  PDR5_RS15900 (PDR5_31990) - 3569496..3569771 (+) 276 WP_007952044.1 peptidylprolyl isomerase -
  PDR5_RS15905 (PDR5_32000) - 3569892..3570824 (-) 933 WP_064596997.1 sugar kinase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41096.96 Da        Isoelectric Point: 6.6452

>NTDB_id=146904 PDR5_RS15890 WP_064596992.1 3565667..3566779(-) (pilU) [Pseudomonas sp. DR 5-09]
MEIDALLKLLASRHGSDLFLSTGAPPSARFDGVLTPLSDQPFKPGEVSSVAASLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHAVNASQALDRA
VNLFPEERRPQLMHALGNNLKALVSQRLVRTVDGQRRAAVEVMLGSPTIADLIKNNQLDDLKVIMEKSGELGMRTFDSAL
HQLVIEGAISEDEALRNADSVNNLRLSLKLNEQNVRADKTSSESWGLLDE

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=146904 PDR5_RS15890 WP_064596992.1 3565667..3566779(-) (pilU) [Pseudomonas sp. DR 5-09]
ATGGAAATCGACGCACTGCTCAAACTGCTGGCGAGCCGTCACGGTTCGGATCTGTTTCTTTCCACCGGTGCACCACCCAG
TGCGCGGTTCGACGGGGTACTGACGCCCTTGAGCGACCAGCCCTTCAAACCCGGCGAGGTGAGCAGTGTCGCCGCCTCCC
TGATGGACGCCGAGCAGCGCCGTGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCTCGAACGGGTATCGGCCGC
TTCCGGGTCAATATTTTCAAACAACGCAACGACGTGTCGATTGTTATCCGTAACGTCAAACTCGACATTCCGCGATTCGA
AGACCTCAAATTACCGCCAGTGCTGCTCGAAACGGTGATGCTCAAGCAGGGGTTGATTCTGTTCGTGGGCGCTACCGACT
CCGGCAAGTCTACCTCCCTGGCGGCGCTGATCGATCATCGCAATCGCCACAGCAGCGGGCACATCATCACCATTGAGGAT
CCGGTGGAGTATATCCATCGACACAAGAAGTCGATCATCAATCAGCGCGAAGTCGGGGTCGACACACGTAGCTTTCATGC
GGCGCTCAAGAACACCTTGCGCCAGGCTCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCATG
CATTGGCGTTCGCCGAAACCGGGCATCTGGTTCTCTCCACATTGCATGCTGTCAACGCCAGTCAGGCGCTGGATCGAGCC
GTCAATTTATTTCCCGAGGAGCGGCGTCCTCAACTGATGCACGCCTTGGGCAACAACCTCAAGGCTCTCGTGTCCCAGCG
TCTGGTACGAACCGTCGATGGGCAGCGGCGCGCGGCCGTGGAAGTGATGCTGGGATCGCCGACGATTGCCGACCTGATCA
AGAACAACCAGTTGGACGATCTGAAAGTCATCATGGAAAAGTCCGGGGAACTTGGTATGCGCACCTTTGACAGCGCCCTG
CATCAACTAGTGATTGAAGGTGCAATCAGTGAAGACGAAGCATTGAGAAATGCTGACTCAGTCAATAACTTGCGTCTTTC
ACTGAAGTTAAATGAGCAAAATGTACGTGCCGACAAAACTTCTTCGGAAAGTTGGGGGCTGCTTGATGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(67-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.261

99.459

0.53
  pilU Acinetobacter baylyi ADP1

52.113

95.946

0.5
  pilU Vibrio cholerae strain A1552

50

94.595

0.473
  pilT Pseudomonas stutzeri DSM 10701

39.826

92.973

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.528

91.622

0.362
  pilT Pseudomonas aeruginosa PAK

38.953

92.973

0.362

Multiple sequence alignment