Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAX06_RS10575 Genome accession   NZ_CP011376
Coordinates   2195199..2196854 (+) Length   551 a.a.
NCBI ID   WP_046697229.1    Uniprot ID   A0AAC8PXR3
Organism   Moraxella bovoculi strain 22581     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2190199..2201854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAX06_RS10545 (AAX06_10545) nusA 2191316..2192797 (-) 1482 WP_046697226.1 transcription termination factor NusA -
  AAX06_RS10550 (AAX06_10550) rimP 2192842..2193339 (-) 498 WP_046697227.1 ribosome maturation factor RimP -
  AAX06_RS10565 (AAX06_10565) secG 2193874..2194200 (-) 327 WP_046697228.1 preprotein translocase subunit SecG -
  AAX06_RS10570 (AAX06_10570) tpiA 2194244..2194981 (-) 738 WP_046697609.1 triose-phosphate isomerase -
  AAX06_RS10575 (AAX06_10575) pilB 2195199..2196854 (+) 1656 WP_046697229.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAX06_RS10580 (AAX06_10580) - 2196847..2198094 (+) 1248 WP_080946133.1 type II secretion system F family protein -
  AAX06_RS10585 (AAX06_10585) - 2198091..2198993 (+) 903 WP_046697230.1 A24 family peptidase -
  AAX06_RS10590 (AAX06_10590) coaE 2199042..2199680 (+) 639 WP_046699412.1 dephospho-CoA kinase -
  AAX06_RS10595 (AAX06_10595) - 2199677..2200213 (-) 537 WP_046697611.1 DUF924 family protein -
  AAX06_RS10600 (AAX06_10600) - 2200248..2200751 (-) 504 WP_080946134.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 551 a.a.        Molecular weight: 60181.61 Da        Isoelectric Point: 6.3771

>NTDB_id=145249 AAX06_RS10575 WP_046697229.1 2195199..2196854(+) (pilB) [Moraxella bovoculi strain 22581]
MNHATTGLARIILDKALINHDVLGAAIDDASAAQISLARHLVRHGVLSSDVIAKTLSESLGIDLVDLDKFNIKELPLHLL
DDALIARHEVLPLGQRDGQIFIATSDPTRAQAMSELGFATNAHITPVLVDENKLVRIMQSLHLGAVLELGEDDHELPDND
GEAMLDAPTVKFVHKTLSDAIIMGASDIHFEPFDKHYRVRFRIDGIMQTIANPPKASGNKISTRLKVMARLDIAERRKPQ
DGRIKFPLHEGKSVDFRVSTTPTLFGEKIVLRLLDSAQALIGIDTLGMNDAQKQLFLDALNRPQGMIVITGPTGSGKTIS
LYTALGILNQPEINIQTVEDPVEIHLDGINQVNINPKIGLDFADVLRAFLRQDPDVIMVGEIRDVQTAEIAIKAAQTGHL
VLSTLHTNSAIDTIIRLKNMGVATFNIASSLSLIIAQRLVRRLCGDCKQPLDIPTQSLIELGFSPDQIGTAKLYKPVGCH
QCKDGYKGRIGIYEMLPITPKLATLIMSDAPTQALSEFAKEHGMITLRQSAMHQVISGITSLQEMERLTGA

Nucleotide


Download         Length: 1656 bp        

>NTDB_id=145249 AAX06_RS10575 WP_046697229.1 2195199..2196854(+) (pilB) [Moraxella bovoculi strain 22581]
ATGAACCACGCCACCACAGGACTTGCTCGAATTATACTAGATAAAGCACTCATCAATCATGACGTGCTTGGGGCTGCCAT
TGATGACGCTAGTGCCGCGCAGATTAGCCTTGCTCGCCATCTAGTGCGACATGGCGTACTGTCCTCCGATGTGATCGCCA
AGACTTTATCTGAGAGTCTGGGCATAGACTTGGTGGATTTGGATAAATTTAACATCAAAGAGCTGCCCTTGCATCTGCTT
GATGATGCGCTCATTGCACGCCACGAGGTGTTACCCCTTGGTCAGCGCGATGGGCAAATTTTTATCGCCACCAGTGACCC
AACACGCGCGCAAGCAATGAGCGAGCTTGGCTTTGCAACCAATGCGCACATCACGCCCGTTCTGGTCGATGAGAATAAAC
TCGTACGCATCATGCAAAGCCTGCATCTAGGCGCGGTGCTAGAGCTTGGCGAGGACGATCATGAACTGCCTGATAATGAC
GGTGAGGCGATGCTTGATGCGCCGACCGTTAAATTCGTCCACAAGACATTAAGTGATGCCATCATCATGGGTGCATCAGA
CATTCATTTTGAGCCGTTCGATAAGCATTATCGGGTAAGATTTCGCATTGATGGCATCATGCAGACCATCGCAAACCCTC
CCAAGGCTTCAGGCAATAAAATCAGTACCCGCCTAAAAGTCATGGCACGTCTCGACATTGCCGAACGACGAAAGCCGCAA
GATGGGCGCATCAAATTTCCCCTACATGAAGGCAAATCGGTTGATTTTCGTGTCAGTACCACGCCCACACTCTTTGGCGA
GAAGATCGTCTTAAGGCTACTAGATTCTGCCCAAGCCCTAATCGGCATTGATACGCTCGGCATGAATGATGCCCAAAAAC
AGCTGTTCCTTGATGCACTAAACCGTCCTCAAGGCATGATCGTCATCACAGGCCCGACAGGCTCTGGTAAGACGATCTCG
CTATACACCGCGCTTGGCATCCTAAATCAACCCGAGATTAACATCCAAACCGTCGAAGACCCTGTGGAAATTCACCTAGA
TGGCATCAATCAGGTCAATATCAACCCAAAGATTGGGCTTGACTTTGCTGATGTGCTAAGAGCTTTCTTGCGTCAAGATC
CCGACGTCATCATGGTGGGCGAGATTCGCGACGTACAGACCGCAGAGATTGCCATTAAGGCCGCTCAGACAGGCCACTTG
GTGCTGTCCACATTACACACCAATTCTGCCATCGACACCATCATTCGCCTAAAGAACATGGGCGTGGCAACCTTTAATAT
CGCAAGCTCTCTAAGTCTCATCATCGCCCAAAGGCTCGTCCGCCGTCTGTGCGGTGACTGTAAGCAGCCCTTAGACATCC
CCACCCAAAGCCTGATTGAGCTTGGCTTTAGCCCAGATCAAATCGGCACAGCCAAGCTATATAAGCCCGTCGGTTGCCAT
CAATGCAAAGACGGCTACAAAGGACGCATAGGCATCTATGAGATGCTGCCCATCACCCCTAAGCTCGCCACGCTCATCAT
GAGCGACGCGCCGACTCAGGCACTTAGCGAATTTGCCAAAGAACACGGCATGATAACCCTTAGACAATCTGCCATGCATC
AGGTCATCAGCGGCATCACAAGCCTACAAGAGATGGAGCGTCTCACAGGTGCCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-139)

Type II/IV secretion system protein (T2SSE)

(175-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

48.227

100

0.494
  pilB Acinetobacter baylyi ADP1

48.128

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

47.687

100

0.486
  pilB Legionella pneumophila strain ERS1305867

49.628

97.641

0.485
  pilB Vibrio campbellii strain DS40M4

46.797

100

0.477
  pilB Vibrio cholerae strain A1552

46.524

100

0.474
  pilF Neisseria gonorrhoeae MS11

44.485

100

0.446
  pilF Thermus thermophilus HB27

37.138

100

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

38.168

95.1

0.363

Multiple sequence alignment