Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAW51_RS22960 Genome accession   NZ_CP011371
Coordinates   5431265..5432983 (+) Length   572 a.a.
NCBI ID   WP_047196465.1    Uniprot ID   A0A0G3BVB1
Organism   Caldimonas brevitalea strain DSM 7029     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5426265..5437983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAW51_RS22930 (AAW51_4605) obgE 5427216..5428316 (-) 1101 WP_047196460.1 GTPase ObgE -
  AAW51_RS22935 (AAW51_4606) - 5428313..5428510 (-) 198 WP_047196461.1 LiaI-LiaF-like domain-containing protein -
  AAW51_RS22940 (AAW51_4607) rpmA 5428719..5428976 (-) 258 WP_047196462.1 50S ribosomal protein L27 -
  AAW51_RS22945 (AAW51_4608) rplU 5429008..5429319 (-) 312 WP_047196463.1 50S ribosomal protein L21 -
  AAW51_RS22950 (AAW51_4609) ispB 5429676..5430668 (+) 993 WP_256365019.1 octaprenyl diphosphate synthase -
  AAW51_RS22960 (AAW51_4610) pilB 5431265..5432983 (+) 1719 WP_047196465.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAW51_RS22965 (AAW51_4611) pilC 5433021..5434241 (+) 1221 WP_047196466.1 type II secretion system F family protein Machinery gene
  AAW51_RS22970 (AAW51_4612) - 5434543..5435397 (+) 855 WP_047196467.1 prepilin peptidase -
  AAW51_RS22975 (AAW51_4613) coaE 5435428..5436048 (+) 621 WP_047196468.1 dephospho-CoA kinase -
  AAW51_RS22980 (AAW51_4614) zapD 5436215..5436967 (+) 753 WP_047196469.1 cell division protein ZapD -
  AAW51_RS22985 (AAW51_4615) - 5436967..5437188 (+) 222 WP_047196470.1 DNA gyrase inhibitor YacG -
  AAW51_RS22990 (AAW51_4616) - 5437206..5437637 (-) 432 WP_047196471.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62252.56 Da        Isoelectric Point: 5.8444

>NTDB_id=145186 AAW51_RS22960 WP_047196465.1 5431265..5432983(+) (pilB) [Caldimonas brevitalea strain DSM 7029]
MDTLAEAPNLTLSGVARVLVNAGKLNAKTAEELAKAAKEKHVSFIAAVIAAGAVSPADLAHALSTAFSLPLIDLNAVDVP
RLPKNVIESKLAAQYQVAVLGKRGSRLFVAGADPTNQEALERIKFATQLTPEWVVVEHDKLLKLLESTGTSAAEALEQMA
NAEFDFDVSEDDTGEKESQDVATDVEDAPVVRFLQKMLIDAINLRASDLHFEPYEQNYRVRFRVDGELREITQPPVAIKE
KLASRIKVISRLDIAEKRVPQDGRMKLKFGNRAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEKEEKDRLLKAI
ERPYGMILVTGPTGSGKTVSLYTCLNMLNRPGVNISTVEDPAEINLPGINQVNVNDKAGLTFSTALKAFLRQDPDVIMVG
EIRDLDTADIAIKAAQTGHMVMSTLHTNDAPTTLTRLRNMGVASFNIASSVILITAQRLARRLCENCKAPATYPREAMLK
AGFQEAELDGGWKPYRAVGCANCNNGYKGRVGLYQVMPISEEIQRIILSDGSALDIAAQAAKEGVRDLRQSGLVKVKLGV
TTLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=145186 AAW51_RS22960 WP_047196465.1 5431265..5432983(+) (pilB) [Caldimonas brevitalea strain DSM 7029]
ATGGACACACTGGCCGAGGCCCCCAACCTGACCCTGTCTGGCGTTGCCCGGGTGCTGGTCAATGCCGGCAAGCTCAATGC
GAAGACGGCAGAAGAGTTGGCGAAGGCCGCCAAAGAGAAACACGTCAGTTTCATCGCGGCCGTGATCGCGGCCGGTGCCG
TGTCGCCGGCCGACCTCGCGCACGCGCTGTCCACGGCCTTCTCGTTGCCGCTGATCGACCTGAATGCGGTCGATGTGCCG
CGGCTGCCCAAGAATGTGATCGAGTCCAAGTTGGCGGCTCAATATCAGGTGGCCGTGCTAGGCAAGCGCGGCAGCCGGCT
GTTCGTCGCCGGCGCCGACCCGACCAACCAGGAGGCGCTGGAGCGCATCAAGTTCGCCACGCAGTTGACGCCCGAGTGGG
TGGTGGTCGAACACGACAAGCTCCTGAAGCTGCTCGAAAGCACGGGCACCTCGGCCGCAGAAGCGCTCGAGCAGATGGCC
AATGCCGAGTTCGATTTCGACGTCTCGGAAGACGATACCGGTGAGAAGGAATCGCAGGACGTCGCGACCGACGTCGAAGA
CGCGCCGGTGGTGCGCTTCCTGCAGAAGATGCTGATCGACGCCATCAACCTGCGTGCGTCCGATTTGCACTTCGAGCCTT
ACGAACAGAACTATCGGGTGCGCTTTCGGGTCGACGGCGAACTGCGCGAGATCACGCAGCCGCCGGTGGCGATCAAGGAA
AAGCTGGCGTCGCGCATCAAGGTCATCTCGCGCCTCGACATCGCTGAAAAGCGTGTGCCGCAAGACGGGCGCATGAAGCT
GAAGTTCGGCAACCGTGCCATCGACTTCCGCGTCAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCGTGATCCGTATTC
TCGACCCGTCCAGCGCCAAGCTGGGCATCGAGGCGCTGGGCTACGAGAAGGAAGAGAAAGACCGATTGCTGAAAGCGATC
GAGCGCCCCTACGGCATGATCCTGGTCACCGGGCCGACGGGTAGCGGCAAGACGGTGTCGCTGTATACCTGTTTGAACAT
GTTGAACCGCCCCGGCGTCAACATCTCGACGGTCGAAGATCCCGCCGAAATCAACCTGCCCGGCATCAACCAGGTTAACG
TCAACGACAAGGCCGGACTGACGTTTTCGACCGCGCTGAAGGCCTTTTTGCGCCAGGATCCCGACGTCATCATGGTCGGC
GAAATCCGCGACCTGGACACGGCCGACATCGCGATCAAGGCCGCCCAAACCGGCCACATGGTGATGTCGACCCTGCACAC
CAACGATGCGCCGACCACGCTGACCCGTCTGCGCAACATGGGTGTGGCCTCGTTCAATATCGCCTCCAGCGTGATCCTGA
TCACGGCACAGCGCTTGGCGCGCCGGCTCTGCGAGAACTGCAAGGCGCCGGCGACTTATCCTCGCGAGGCGATGCTGAAG
GCCGGCTTCCAGGAAGCGGAACTCGACGGCGGCTGGAAGCCCTACCGCGCCGTGGGCTGCGCCAATTGCAACAATGGGTA
TAAAGGTCGTGTCGGTCTTTACCAAGTGATGCCCATCTCGGAAGAGATCCAGCGCATCATCCTGTCCGACGGCAGCGCCC
TGGACATTGCTGCGCAGGCCGCGAAAGAAGGCGTGCGCGACCTCCGGCAGTCCGGACTGGTGAAAGTGAAGTTGGGCGTC
ACAACGCTCGAAGAAGTGATCTCGGTCACAAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-147)

Type II/IV secretion system protein (T2SSE)

(196-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G3BVB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.878

100

0.551
  pilB Acinetobacter baylyi ADP1

53.996

98.427

0.531
  pilF Neisseria gonorrhoeae MS11

52.9

99.476

0.526
  pilB Legionella pneumophila strain ERS1305867

51.146

99.126

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

46.018

98.776

0.455
  pilB Vibrio cholerae strain A1552

46.835

96.678

0.453
  pilB Vibrio campbellii strain DS40M4

46.237

97.552

0.451
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.566

92.657

0.376
  pilF Thermus thermophilus HB27

37.148

99.301

0.369

Multiple sequence alignment