Detailed information NTG0144132

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: XM40_RS11965
• Genome accession: NZ_CP011278
• Coordinates: 2526097..2526711 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain L-S60
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2521097..2531711

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XM40_RS11940 (XM40_11940)	rpsT	2521397..2521663 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
XM40_RS11945 (XM40_11945)	holA	2521680..2522721 (-)	1042	Protein_2366	DNA polymerase III subunit delta	-
XM40_RS19715	-	2522761..2522915 (-)	155	Protein_2367	hypothetical protein	-
XM40_RS19295 (XM40_11950)	-	2522956..2523090 (+)	135	WP_003152870.1	YqzM family protein	-
XM40_RS11955 (XM40_11955)	comEC	2523109..2525460 (-)	2352	WP_043867296.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
XM40_RS11960 (XM40_11960)	-	2525461..2526030 (-)	570	WP_003152868.1	ComE operon protein 2	-
XM40_RS11965 (XM40_11965)	comEA	2526097..2526711 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
XM40_RS11970 (XM40_11970)	comER	2526770..2527591 (+)	822	WP_043867298.1	late competence protein ComER	-
XM40_RS11975 (XM40_11975)	-	2527660..2528397 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
XM40_RS11980 (XM40_11980)	rsfS	2528394..2528750 (-)	357	WP_003152864.1	ribosome silencing factor	-
XM40_RS11985 (XM40_11985)	yqeK	2528747..2529328 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
XM40_RS11990 (XM40_11990)	-	2529318..2529887 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
XM40_RS11995 (XM40_11995)	yhbY	2529898..2530188 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
XM40_RS12000 (XM40_12000)	aroE	2530182..2531024 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=144132 XM40_RS11965 WP_043867297.1 2526097..2526711(-) (comEA) [Bacillus velezensis strain L-S60]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=144132 XM40_RS11965 WP_043867297.1 2526097..2526711(-) (comEA) [Bacillus velezensis strain L-S60]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363