Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   APT60_RS09455 Genome accession   NZ_CP013989
Coordinates   2040283..2041791 (+) Length   502 a.a.
NCBI ID   WP_003091377.1    Uniprot ID   A0A0H2ZC25
Organism   Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2035283..2046791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APT60_RS09435 (APT60_09435) - 2035313..2036524 (+) 1212 WP_003113933.1 O-succinylhomoserine sulfhydrylase -
  APT60_RS09440 (APT60_09440) - 2036521..2037288 (+) 768 WP_003103539.1 SDR family oxidoreductase -
  APT60_RS09445 (APT60_09445) xcpQ 2037375..2039351 (-) 1977 WP_003103536.1 GspD family T2SS secretin variant XcpQ -
  APT60_RS09450 (APT60_09450) xcpP 2039356..2040063 (-) 708 WP_003119750.1 type II secretion system protein N -
  APT60_RS09455 (APT60_09455) pilB 2040283..2041791 (+) 1509 WP_003091377.1 GspE family T2SS ATPase variant XcpR Machinery gene
  APT60_RS09460 (APT60_09460) xcpS 2041791..2043008 (+) 1218 WP_059349539.1 GspF family T2SS innner membrane protein variant XcpS -
  APT60_RS09465 (APT60_09465) xcpT 2043013..2043459 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  APT60_RS09470 (APT60_09470) xcpU 2043466..2043984 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  APT60_RS09475 (APT60_09475) xcpV 2043981..2044370 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  APT60_RS09480 (APT60_09480) xcpW 2044367..2045080 (+) 714 WP_003110067.1 GspJ family T2SS minor pseudopilin variant XcpW -
  APT60_RS09485 (APT60_09485) xcpX 2045077..2046078 (+) 1002 WP_003103524.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55524.73 Da        Isoelectric Point: 6.2074

>NTDB_id=143739 APT60_RS09455 WP_003091377.1 2040283..2041791(+) (pilB) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLKALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=143739 APT60_RS09455 WP_003091377.1 2040283..2041791(+) (pilB) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAAGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATACGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAACGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGTGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAACACTTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAGCTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCGAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACTACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.532

97.012

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.249

94.422

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.207

89.442

0.386
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367