Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AzCIB_RS18185 Genome accession   NZ_CP011072
Coordinates   4063004..4064719 (+) Length   571 a.a.
NCBI ID   WP_050417189.1    Uniprot ID   -
Organism   Azoarcus sp. CIB     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4058004..4069719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AzCIB_RS18160 (AzCIB_3633) - 4058362..4059135 (-) 774 WP_232299262.1 SprT family zinc-dependent metalloprotease -
  AzCIB_RS18165 (AzCIB_3634) - 4059132..4059884 (-) 753 WP_050417185.1 molybdopterin-binding protein -
  AzCIB_RS18170 (AzCIB_3635) - 4059888..4060658 (-) 771 WP_050417186.1 EI24 domain-containing protein -
  AzCIB_RS18175 (AzCIB_3636) ffh 4060672..4062030 (-) 1359 WP_050417187.1 signal recognition particle protein -
  AzCIB_RS18180 (AzCIB_3637) ccsA 4062073..4062900 (+) 828 WP_050417188.1 cytochrome c biogenesis protein CcsA -
  AzCIB_RS18185 (AzCIB_3638) pilB 4063004..4064719 (+) 1716 WP_050417189.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AzCIB_RS18190 (AzCIB_3639) - 4064736..4065967 (+) 1232 Protein_3666 type II secretion system F family protein -
  AzCIB_RS18195 (AzCIB_3641) pilD 4066022..4066873 (+) 852 WP_050417190.1 A24 family peptidase Machinery gene
  AzCIB_RS18200 (AzCIB_3642) - 4067000..4067263 (+) 264 WP_050417191.1 zinc ribbon domain-containing protein -
  AzCIB_RS18205 (AzCIB_3643) - 4067279..4067905 (+) 627 WP_050417192.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62204.35 Da        Isoelectric Point: 5.2369

>NTDB_id=142031 AzCIB_RS18185 WP_050417189.1 4063004..4064719(+) (pilB) [Azoarcus sp. CIB]
MAANPQTALSGLARALIQHGRLSEADAIACTKSAAAVPNGFILELAQRKLLNTKAVAWFAAETFGYPLLDISSVDRASIP
RDAIDRKLMAKHQVVALGKRQNRLTVATADPSNMRALDEIRFQTGMQVDLIVVEADRLARLAEALSESTEDTLKDLTGEE
FDMDLLQDQEATEQASEDASAEVDDAPVVKFIQKILIDAINEGASDVHFEPYEKYYRIRVRTDGILREIAQPPLILKEKI
AARIKVISRLDISEKRVPQDGRMKLVLSKNKSIDFRVSSLPTLHGEKIVMRILDASSAMLGVDALGYEPEQKKVLLDAIE
RPYGMILVTGPTGSGKTVSLYTCLNILNKAGVNISTAEDPAEINLAGINQVNVNDKAGLTFAASLRAFLRQDPDVIMVGE
VRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVILITAQRLARRLCTCKQPVDIPVEALLEAG
FSGEDLDGSWRPYGPVGCERCKGSGYKGRLGIYQVMPISEEIAHIIMTNGNSMDIAAQAQREGVRDLRQSGLLKVKQGAT
SLEEVLATTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=142031 AzCIB_RS18185 WP_050417189.1 4063004..4064719(+) (pilB) [Azoarcus sp. CIB]
ATGGCAGCAAACCCCCAGACCGCCCTGAGCGGACTCGCGCGAGCACTGATCCAGCACGGCCGGCTCTCCGAAGCCGATGC
GATTGCGTGCACCAAGAGCGCGGCAGCCGTCCCGAACGGCTTCATCCTCGAACTTGCGCAGCGCAAGCTCCTGAACACCA
AGGCCGTAGCCTGGTTCGCAGCCGAAACGTTCGGCTATCCGCTGCTCGACATCTCGTCCGTGGATCGCGCCAGCATCCCC
CGCGATGCGATCGACCGCAAGCTGATGGCCAAGCATCAGGTCGTCGCGCTGGGCAAACGCCAGAACCGCCTCACCGTTGC
GACGGCCGACCCGTCCAACATGCGCGCGCTCGACGAGATCCGCTTCCAGACCGGGATGCAGGTCGACCTCATCGTCGTCG
AAGCCGACCGTCTGGCACGCCTTGCGGAAGCGCTGTCCGAATCGACCGAAGACACGCTCAAGGATCTCACGGGCGAAGAG
TTCGACATGGACCTCCTGCAGGACCAGGAGGCCACGGAGCAGGCGAGCGAGGACGCCTCCGCGGAAGTCGATGATGCCCC
CGTCGTCAAATTCATCCAGAAGATCCTGATCGACGCGATCAACGAAGGCGCGTCCGACGTCCACTTCGAGCCGTACGAAA
AGTACTACCGCATCCGCGTCCGTACCGACGGCATCCTGCGGGAAATCGCCCAGCCGCCGTTGATCCTGAAGGAAAAGATC
GCCGCGCGCATCAAGGTCATCTCGCGCCTGGACATTTCCGAGAAGCGCGTCCCGCAGGACGGACGCATGAAGCTCGTCCT
ATCGAAGAACAAGTCGATCGACTTCCGCGTGTCCTCGCTGCCGACCCTGCATGGCGAGAAGATCGTCATGCGTATTCTCG
ATGCTTCCTCGGCAATGCTGGGGGTCGACGCACTCGGTTACGAGCCGGAACAGAAAAAAGTGCTGCTCGACGCGATCGAG
CGTCCGTACGGAATGATTCTCGTGACCGGACCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCTGCCTGAACATCCT
CAACAAGGCGGGCGTCAACATCTCGACCGCGGAGGACCCCGCGGAAATCAACCTGGCCGGCATCAATCAGGTCAACGTGA
ACGACAAGGCCGGTCTGACCTTCGCCGCGTCGCTGCGCGCCTTTCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAA
GTTCGCGATCTCGAGACCGCCGAAATCTCGATCAAGGCGGCGCAGACCGGCCACCTGGTGTTATCGACGCTGCACACCAA
CGACGCCCCGACCACGCTCGAGCGCCTGCGCAACATGGGCGTCGCACCGTTCAACATCGCCTCCTCGGTGATCCTGATCA
CCGCCCAGCGCCTCGCGCGGCGCCTGTGCACGTGCAAGCAACCGGTGGACATCCCGGTAGAAGCCTTGCTCGAAGCGGGA
TTCAGCGGCGAAGACCTCGACGGCAGCTGGCGCCCGTACGGCCCTGTCGGGTGCGAACGCTGCAAGGGCAGCGGCTACAA
GGGGCGCCTGGGTATCTACCAGGTCATGCCCATTTCCGAAGAAATTGCCCATATCATCATGACCAACGGCAATTCGATGG
ACATCGCGGCTCAAGCTCAGCGCGAAGGCGTGCGCGACCTGCGCCAGTCCGGTCTGCTGAAAGTGAAACAGGGTGCGACC
TCGCTTGAGGAAGTGCTCGCCACCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.007

99.124

0.555
  pilB Acinetobacter baumannii D1279779

55.3

99.124

0.548
  pilB Legionella pneumophila strain ERS1305867

53.136

100

0.534
  pilF Neisseria gonorrhoeae MS11

56.061

92.469

0.518
  pilB Vibrio cholerae strain A1552

49.074

94.571

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

50.383

91.419

0.461
  pilB Vibrio campbellii strain DS40M4

49.123

89.842

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.678

92.995

0.378
  pilF Thermus thermophilus HB27

38.93

94.921

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

42.052

87.04

0.366

Multiple sequence alignment