Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PTET_RS13890 Genome accession   NZ_CP011041
Coordinates   2978120..2979796 (+) Length   558 a.a.
NCBI ID   WP_096038788.1    Uniprot ID   A0AA37S261
Organism   Pseudoalteromonas tetraodonis strain GFC     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2973120..2984796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTET_RS13865 (PTET_a3097) nadC 2973148..2973993 (+) 846 WP_096038783.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PTET_RS13870 (PTET_a3098) - 2974514..2974951 (+) 438 WP_407681291.1 pilin -
  PTET_RS13875 (PTET_a3099) pilC 2974998..2976218 (+) 1221 WP_096038785.1 type II secretion system F family protein Machinery gene
  PTET_RS13880 (PTET_a3100) pilD 2976443..2977354 (+) 912 WP_096038786.1 prepilin peptidase Machinery gene
  PTET_RS13885 (PTET_a3101) coaE 2977357..2977986 (+) 630 WP_096038787.1 dephospho-CoA kinase -
  PTET_RS13890 (PTET_a3102) pilB 2978120..2979796 (+) 1677 WP_096038788.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PTET_RS13895 (PTET_a3104) yacG 2979862..2980092 (+) 231 WP_008466423.1 DNA gyrase inhibitor YacG -
  PTET_RS13900 (PTET_a3105) - 2980435..2980899 (-) 465 WP_008114914.1 periplasmic heavy metal sensor -
  PTET_RS13905 (PTET_a3106) - 2981067..2981756 (+) 690 WP_008114913.1 response regulator transcription factor -
  PTET_RS13910 (PTET_a3108) - 2981734..2983161 (+) 1428 WP_096038789.1 ATP-binding protein -
  PTET_RS13915 (PTET_a3109) - 2983151..2984095 (+) 945 WP_096038790.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62372.67 Da        Isoelectric Point: 6.1023

>NTDB_id=141713 PTET_RS13890 WP_096038788.1 2978120..2979796(+) (pilB) [Pseudoalteromonas tetraodonis strain GFC]
MNINSPLLRKFITLGRVDTDTVKTRQQDFASTAELISKCGKIDSKDLAEQCIDLFRVPFFDLKDFNVAQISAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYVQLDNKIEQLLDATGSLNLSEDEFKEFAELDSEQ
HKEHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKVTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELIRQGFSEQQIQDMVL
YAPKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGFNNIRLSGLRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=141713 PTET_RS13890 WP_096038788.1 2978120..2979796(+) (pilB) [Pseudoalteromonas tetraodonis strain GFC]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTCGGCCGGGTTGATACTGATACCGTAAAAACACGGCAGCA
AGATTTTGCGTCAACTGCAGAGCTTATCTCTAAATGTGGAAAAATAGACAGTAAAGATTTAGCTGAGCAATGCATTGATT
TATTTAGAGTGCCTTTTTTTGATTTAAAAGATTTTAATGTTGCACAAATATCAGCTGAGCTGGTTAAAGAAAAACTGATC
CGCAAACACCATATTTTGCCTTTGGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCAGATCCTACCGATTATGGTGC
CTTCGAAAACTTTGAATTTAGCACGGGTTTATCGTGCGAAGTAGTTGTGGTTGACTACGTCCAGCTCGATAACAAAATTG
AGCAATTACTCGATGCCACTGGCAGTTTAAATTTAAGTGAAGACGAATTTAAAGAGTTCGCTGAACTCGATTCTGAGCAG
CACAAAGAACACACTCCTAAAGACGATGACAAAGACGACGCCCCGATTATTGTCTACATTAATAAAATTTTAATGGATGC
AATAAAAAAAGGCGCCTCAGATTTACACTTTGAACCTTATGAACACAAATACCGTATTCGCTTTCGTATTGATGGTATTT
TGCACGAAGTAGCCAGTCCGCCAAATACTTTAGCCAGTAAATTATCAGCGCGAATAAAAGTAATGTCGCGACTGGATATT
GCAGAAAAGCGTAAACCACAAGATGGCCGTATTAAGCTTAAGGTAACAGAGCGTAAAAGCATTGATTTTCGTGTTAGTAC
CATGCCAACTTTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCCAGTGCCATGCTAGGTATTGATGTATTAG
GCTATGAACCCGAGCAGAAAAAACTATACCTCGATGCACTTGAGCAACCGCAAGGAATGATTTTGGTTACAGGCCCAACC
GGTTCAGGTAAAACGGTTTCTTTGTATACGGGCTTAAATATACTAAATCAGCCGGAGCGGAATATTAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATAAATACTAAAGCAGATATGACTTTTGCTAATGCACTGC
GGGCGTTTTTACGTCAAGATCCCGATGTGGTGATGGTGGGTGAAATACGTGATTTAGAAACTGCAGAAATATCAATTAAA
GCGGCGCAAACAGGTCACTTGGTGCTCTCAACACTGCATACCAATTCAGCGCCAGAAACGATTACTCGTTTGTTAAATAT
GGGTGTACCCGCGTATAATGTGGCCAGTTCTATTAGTTTAATTATAGCTCAGCGTTTAGCCCGAAGGTTATGCTCTAAAT
GTAAAACACCAGAGCAGTTACCTCGCGAAGAGCTTATACGCCAAGGCTTTAGTGAACAACAAATTCAAGACATGGTTTTA
TACGCACCTAAAGGCTGTGAAAACTGTACCGATGGCTACAAAGGCCGTGTTGGTATTTACGAAGTAATGAAAATTACGCC
TGAAATTGCCCAAATTATAATGCGCGGCGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGGTTTAATAACA
TACGCTTATCAGGTCTTCGAAAAGCCGCAGAGGGTGTGACCTCACTTGCTGAGATAAACCGTGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.778

96.774

0.559
  pilB Acinetobacter baylyi ADP1

58.614

90.502

0.53
  pilB Legionella pneumophila strain ERS1305867

55.078

91.756

0.505
  pilB Vibrio cholerae strain A1552

52.06

95.699

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.311

95.699

0.491
  pilB Vibrio campbellii strain DS40M4

50.749

95.699

0.486
  pilF Neisseria gonorrhoeae MS11

49.425

93.548

0.462
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.996

92.832

0.362

Multiple sequence alignment