Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TQ33_RS08490 Genome accession   NZ_CP010975
Coordinates   1885779..1887491 (-) Length   570 a.a.
NCBI ID   WP_046561675.1    Uniprot ID   -
Organism   Kangiella geojedonensis strain YCS-5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1880779..1892491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TQ33_RS08465 (TQ33_1680) nadC 1881233..1882102 (+) 870 WP_046561670.1 carboxylating nicotinate-nucleotide diphosphorylase -
  TQ33_RS08470 (TQ33_1681) zapD 1882103..1882870 (-) 768 WP_046561671.1 cell division protein ZapD -
  TQ33_RS08475 (TQ33_1682) coaE 1882875..1883495 (-) 621 WP_046561672.1 dephospho-CoA kinase -
  TQ33_RS08480 (TQ33_1683) - 1883501..1884409 (-) 909 WP_046561673.1 A24 family peptidase -
  TQ33_RS08485 (TQ33_1684) pilC 1884440..1885687 (-) 1248 WP_046561674.1 type II secretion system F family protein Machinery gene
  TQ33_RS08490 (TQ33_1685) pilB 1885779..1887491 (-) 1713 WP_046561675.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TQ33_RS08495 (TQ33_1686) - 1887560..1889269 (-) 1710 WP_046561676.1 PglL family O-oligosaccharyltransferase -
  TQ33_RS08500 (TQ33_1687) - 1889325..1889756 (-) 432 WP_046561677.1 pilin -
  TQ33_RS08505 yacG 1890282..1890479 (+) 198 WP_052735260.1 DNA gyrase inhibitor YacG -
  TQ33_RS08510 (TQ33_1688) - 1890480..1890857 (-) 378 WP_046561678.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62545.70 Da        Isoelectric Point: 4.9910

>NTDB_id=141007 TQ33_RS08490 WP_046561675.1 1885779..1887491(-) (pilB) [Kangiella geojedonensis strain YCS-5]
MALVETGLTGLAHMLVAEGLIDKEKVKDAIERAKEEKIGLIDWLVNSGVVQGADAVKAQGVNFGAPYFDASSINPDNLPK
DIIDLNMMRKHRVLPLYKRGTRLFVGVADATNLKSLEEVRFQTNCSIEAVLVEPSKLDSLVNKLIDEKESESLSDFDDAD
LDNIDLDAVDEGQGGDIDEGAERDDAPVVKFVKKMLVDAIRKGASDLHFEPYEKKYRVRFRIDGLLHEVASPPIQLSSRI
SARLKVLSNLDISERRVPQDGRVKLKLSKTKAIDFRVNTLPTLFGEKIVMRILDPTSAMLGVDALGFEQQQKKHFMEAIS
KPQGMVLVTGPTGSGKTVTLYTGVNILNDEHKNISTAEDPVEINLVGVNQVNVNVKAGLTFASALRAFLRQDPDIVLVGE
IRDLETAEIAIKASQTGHLVLSTLHTNSAPETLTRLVNMGVAPFNIATTVNLVVAQRLARRLCEHCKKPADLPDEALLEE
GFTQEELNDLTIYEPVGCDKCTLGYKGRVGLFQVMPVSEETGRIIMEGGNAIDIADQAKKENIPDLRRAGLNKIKLGATS
LEEVNRVTQE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=141007 TQ33_RS08490 WP_046561675.1 1885779..1887491(-) (pilB) [Kangiella geojedonensis strain YCS-5]
ATGGCATTAGTTGAAACAGGCCTTACAGGTTTAGCCCACATGCTTGTAGCAGAAGGGCTAATAGATAAAGAAAAAGTTAA
AGATGCAATTGAGCGAGCTAAAGAAGAAAAAATTGGCCTGATTGATTGGTTAGTTAATTCTGGCGTAGTTCAGGGGGCCG
ATGCCGTAAAAGCACAAGGAGTTAATTTCGGTGCGCCTTATTTCGACGCATCTTCCATAAACCCTGACAATTTACCCAAG
GATATTATCGATCTAAATATGATGCGAAAGCATCGTGTTCTTCCGCTTTATAAACGCGGAACCCGTTTATTTGTGGGGGT
AGCAGATGCTACTAATCTTAAGAGCCTAGAAGAAGTCAGGTTCCAAACCAATTGCTCTATTGAAGCTGTATTGGTGGAAC
CTTCAAAGTTAGACTCACTCGTTAATAAGCTGATTGATGAAAAAGAAAGTGAGTCATTATCCGATTTTGATGACGCCGAT
CTCGATAATATTGACTTAGATGCCGTGGATGAGGGGCAAGGCGGAGACATTGATGAGGGAGCTGAAAGAGATGATGCTCC
TGTTGTTAAATTTGTAAAAAAAATGTTAGTGGATGCCATTCGAAAAGGGGCATCGGATTTGCACTTCGAACCTTACGAAA
AAAAATACAGAGTTAGATTTCGTATTGATGGCCTGTTACATGAGGTTGCAAGTCCGCCAATTCAGTTATCTTCACGTATA
TCAGCTCGTCTAAAAGTACTATCTAACTTGGATATTTCTGAACGTCGAGTACCCCAAGATGGTCGTGTGAAGTTAAAGCT
ATCGAAAACCAAGGCAATTGATTTTCGTGTGAATACTCTGCCCACGCTATTCGGCGAAAAGATTGTTATGCGTATTCTCG
ATCCAACAAGTGCCATGTTAGGTGTTGATGCGCTAGGTTTTGAACAACAGCAAAAGAAACATTTTATGGAGGCTATCAGC
AAGCCACAAGGAATGGTTTTAGTCACAGGGCCTACTGGTTCTGGTAAGACAGTTACGCTGTATACAGGCGTCAATATTTT
AAACGATGAACACAAAAACATTTCTACTGCAGAAGACCCTGTAGAAATTAACCTAGTCGGTGTAAATCAGGTTAATGTTA
ATGTGAAAGCTGGGCTAACTTTCGCTTCGGCATTAAGAGCTTTCTTACGTCAGGATCCTGACATTGTACTGGTCGGTGAG
ATACGTGACTTAGAAACTGCTGAAATTGCAATTAAAGCGTCCCAAACGGGTCACTTAGTACTATCTACTTTGCACACAAA
CTCGGCACCAGAAACCTTAACGCGATTGGTAAACATGGGCGTTGCGCCGTTTAATATAGCGACTACCGTTAATCTTGTTG
TAGCTCAGCGTTTGGCTAGAAGGCTCTGTGAACACTGCAAAAAACCGGCAGATTTGCCCGATGAGGCATTATTAGAAGAA
GGTTTTACGCAAGAAGAGCTGAACGATCTCACAATTTATGAACCGGTTGGTTGCGATAAATGTACATTAGGTTATAAAGG
TCGTGTGGGTTTATTCCAAGTAATGCCAGTATCTGAGGAGACAGGACGTATTATCATGGAGGGTGGTAACGCAATTGATA
TCGCGGATCAGGCTAAAAAAGAAAATATACCTGATTTAAGAAGAGCTGGTTTGAATAAAATTAAGTTAGGGGCTACCAGT
CTAGAAGAAGTAAATCGCGTCACTCAAGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.89

99.298

0.565
  pilB Acinetobacter baylyi ADP1

56.283

99.123

0.558
  pilB Legionella pneumophila strain ERS1305867

54.196

100

0.544
  pilF Neisseria gonorrhoeae MS11

52.165

89.123

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

45.55

100

0.458
  pilB Vibrio cholerae strain A1552

46.113

99.298

0.458
  pilB Vibrio campbellii strain DS40M4

45.648

98.772

0.451
  pilF Thermus thermophilus HB27

38.129

97.544

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.143

98.246

0.365

Multiple sequence alignment