Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NHmuc_RS05780 Genome accession   NZ_CP013479
Coordinates   1217942..1219351 (+) Length   469 a.a.
NCBI ID   WP_034009084.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NHmuc     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1212942..1224351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHmuc_RS05765 (NHmuc_1130) gspL 1213746..1214957 (+) 1212 WP_004348155.1 type II secretion system protein GspL -
  NHmuc_RS05770 (NHmuc_1131) - 1214944..1215537 (+) 594 WP_003112724.1 type II secretion system protein M -
  NHmuc_RS05775 (NHmuc_1132) gspD 1215534..1217945 (+) 2412 WP_009315212.1 type II secretion system secretin GspD -
  NHmuc_RS05780 (NHmuc_1133) pilB 1217942..1219351 (+) 1410 WP_034009084.1 type II secretion system ATPase GspE Machinery gene
  NHmuc_RS05785 (NHmuc_1134) pilC 1219351..1220565 (+) 1215 WP_003112721.1 type II secretion system inner membrane protein GspF Machinery gene
  NHmuc_RS05790 (NHmuc_1136) lapA 1221079..1222185 (+) 1107 WP_003116534.1 alkaline phosphatase LapA -
  NHmuc_RS05795 (NHmuc_1137) - 1222271..1223383 (+) 1113 WP_003112720.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50974.37 Da        Isoelectric Point: 5.7943

>NTDB_id=140996 NHmuc_RS05780 WP_034009084.1 1217942..1219351(+) (pilB) [Pseudomonas aeruginosa strain NHmuc]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHAPASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFEPHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=140996 NHmuc_RS05780 WP_034009084.1 1217942..1219351(+) (pilB) [Pseudomonas aeruginosa strain NHmuc]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGGACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCCGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAGCTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTCACTGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGCCCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCTTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCAGTGACGCGGCTGGTGGACATGGGGGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCTTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCGGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373

Multiple sequence alignment