Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FORC22_RS00635 Genome accession   NZ_CP013248
Coordinates   137797..139299 (+) Length   500 a.a.
NCBI ID   WP_071651737.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_022     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 132797..144299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC22_RS00615 (FORC22_0113) hslO 133261..134136 (-) 876 WP_005465326.1 Hsp33 family molecular chaperone HslO -
  FORC22_RS00620 (FORC22_0114) hslR 134168..134554 (-) 387 WP_005465328.1 ribosome-associated heat shock protein Hsp15 -
  FORC22_RS00625 (FORC22_0115) gspC 134810..135733 (+) 924 WP_005465330.1 type II secretion system protein GspC -
  FORC22_RS00630 (FORC22_0116) gspD 135770..137797 (+) 2028 WP_005478663.1 type II secretion system secretin GspD -
  FORC22_RS00635 (FORC22_0117) pilB/pilB1 137797..139299 (+) 1503 WP_071651737.1 type II secretion system ATPase GspE Machinery gene
  FORC22_RS00640 (FORC22_0118) gspF 139299..140516 (+) 1218 WP_005465194.1 type II secretion system inner membrane protein GspF -
  FORC22_RS00645 (FORC22_0119) gspG 140578..141021 (+) 444 WP_005465191.1 type II secretion system major pseudopilin GspG -
  FORC22_RS00650 (FORC22_0120) gspH 141052..141648 (+) 597 WP_023624183.1 type II secretion system minor pseudopilin GspH -
  FORC22_RS00655 (FORC22_0121) gspI 141635..142024 (+) 390 WP_025500561.1 type II secretion system minor pseudopilin GspI -
  FORC22_RS00660 (FORC22_0122) gspJ 142011..142694 (+) 684 WP_071651738.1 type II secretion system minor pseudopilin GspJ -
  FORC22_RS00665 (FORC22_0123) gspK 142687..143697 (+) 1011 WP_071651739.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55801.91 Da        Isoelectric Point: 5.7919

>NTDB_id=139853 FORC22_RS00635 WP_071651737.1 137797..139299(+) (pilB/pilB1) [Vibrio parahaemolyticus strain FORC_022]
MVDMLDTAPSMRRLPFSFANRFKLVLETEHPERPPILYYVEPLNPAALVEVRRVLKRSFVPQAIDKESFDKKLTEAYQRD
SSEARQLMEDIGADSDDFFSLAEELPHDEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGVLRE
VLTPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTP
SVHDNFRHLIGRPHGIILVTGPTGSGKSTTLYAGLQEINSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAIL
RQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCSDCKEP
YEADSEQKKLFGMAESESLILHRAKGCEECNQKGYRGRTGIHELVLVDETVQELIHREAGEQEVEKAVRNHTPSIRSDGL
SKVRRGITSLEEVMRVTKEA

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=139853 FORC22_RS00635 WP_071651737.1 137797..139299(+) (pilB/pilB1) [Vibrio parahaemolyticus strain FORC_022]
ATGGTAGATATGTTAGACACTGCGCCAAGTATGCGTCGTCTGCCATTCAGTTTTGCCAATCGCTTTAAACTGGTTTTGGA
GACTGAGCATCCTGAGCGTCCTCCAATTCTTTACTACGTGGAGCCGCTAAATCCTGCGGCTCTGGTTGAAGTACGTCGTG
TGCTTAAGCGCAGCTTCGTGCCTCAAGCCATCGACAAAGAATCCTTCGATAAAAAGCTGACTGAAGCGTATCAACGTGAT
TCATCAGAAGCTCGTCAACTGATGGAAGATATCGGTGCCGACAGTGACGATTTCTTCTCGTTGGCAGAAGAGCTACCGCA
TGATGAGGACTTGTTAGAGTCGGAAGACGATGCGCCAATCATCAAATTGATCAATGCCATGTTGGGTGAAGCGATCAAAG
AAGGTGCGTCCGATATCCATATCGAAACGTTTGAAAAGACGCTTTCTATCCGCTTCCGTGTCGACGGCGTTCTGCGTGAA
GTCTTGACGCCGAGTCGTAAGCTTGCGCCGTTGCTGGTGTCGCGTGTGAAGGTTATGGCGAAGCTCGATATTGCTGAAAA
GCGTGTGCCTCAAGATGGTCGTATCTCGCTACGTATTGGTGGCCGAGCGGTCGACGTGCGTGTTTCTACCATGCCATCCT
CTCACGGTGAACGAGTGGTAATGCGTCTGTTAGATAAAAACGCAACGCGCCTTGACCTGCATAGTTTGGGCATGACTCCT
TCGGTACACGACAACTTCCGTCACCTCATTGGTCGCCCACACGGTATCATCTTGGTGACCGGCCCTACGGGTTCTGGTAA
ATCGACCACTTTGTACGCCGGCTTGCAAGAAATCAACAGCAACGAGCGCAACATTCTGACGGTGGAAGACCCAATCGAAT
TTGATATCGATGGCATTGGCCAGACCCAAGTTAACCCGAAAGTCGACATGACATTTGCGCGTGGTCTACGTGCGATCTTA
CGTCAGGACCCGGATGTGGTGATGGTTGGTGAGATCCGTGACTTAGAAACCGCGCAAATTGCAGTGCAAGCGTCTTTGAC
TGGTCACTTGGTAATGTCGACGCTGCATACCAACACCGCTGTTGGTGCGATCACGCGTCTGCGTGATATGGGCATTGAAC
CATTTTTGATCTCCTCTTCACTGCTTGGTGTGCTAGCGCAGCGTCTGGTTCGTACGCTTTGTTCTGACTGTAAAGAACCT
TACGAAGCGGACAGCGAACAGAAAAAATTGTTTGGCATGGCAGAAAGTGAATCGCTCATTCTACATCGCGCGAAAGGCTG
TGAAGAGTGTAACCAAAAAGGTTATCGCGGACGTACCGGCATTCATGAATTAGTGCTTGTCGATGAAACGGTTCAAGAGC
TGATTCACCGCGAAGCAGGTGAGCAAGAAGTGGAAAAAGCCGTTCGTAATCATACGCCAAGTATTCGTAGTGATGGTCTA
AGCAAAGTACGTCGTGGTATTACGTCACTTGAAGAAGTGATGCGAGTGACGAAGGAAGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(126-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.939

100

0.45
  pilB Legionella pneumophila strain ERS1305867

43.967

97.8

0.43
  pilF Thermus thermophilus HB27

44.161

94.2

0.416
  pilB Acinetobacter baumannii D1279779

42.827

96.2

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

46.699

81.8

0.382
  pilB Vibrio campbellii strain DS40M4

46.699

81.8

0.382
  pilB Vibrio cholerae strain A1552

47.727

79.2

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.692

78

0.372
  pilF Neisseria gonorrhoeae MS11

46.193

78.8

0.364
  pilB Acinetobacter baylyi ADP1

46.53

77.8

0.362