Detailed information NTG0139272

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: SB45_RS11950
• Genome accession: NZ_CP010556
• Coordinates: 2529272..2529886 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain L-H15
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2524272..2534886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SB45_RS11925 (SB45_11925)	rpsT	2524572..2524838 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
SB45_RS11930 (SB45_11930)	holA	2524855..2525896 (-)	1042	Protein_2367	DNA polymerase III subunit delta	-
SB45_RS19690	-	2525936..2526090 (-)	155	Protein_2368	hypothetical protein	-
SB45_RS19270 (SB45_11935)	-	2526131..2526265 (+)	135	WP_003152870.1	YqzM family protein	-
SB45_RS11940 (SB45_11940)	comEC	2526284..2528635 (-)	2352	WP_043867296.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SB45_RS11945 (SB45_11945)	-	2528636..2529205 (-)	570	WP_003152868.1	ComE operon protein 2	-
SB45_RS11950 (SB45_11950)	comEA	2529272..2529886 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
SB45_RS11955 (SB45_11955)	comER	2529945..2530766 (+)	822	WP_043867298.1	late competence protein ComER	-
SB45_RS11960 (SB45_11960)	-	2530835..2531572 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
SB45_RS11965 (SB45_11965)	rsfS	2531569..2531925 (-)	357	WP_003152864.1	ribosome silencing factor	-
SB45_RS11970 (SB45_11970)	yqeK	2531922..2532503 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SB45_RS11975 (SB45_11975)	-	2532493..2533062 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
SB45_RS11980 (SB45_11980)	yhbY	2533073..2533363 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
SB45_RS11985 (SB45_11985)	aroE	2533357..2534199 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=139272 SB45_RS11950 WP_043867297.1 2529272..2529886(-) (comEA) [Bacillus velezensis strain L-H15]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=139272 SB45_RS11950 WP_043867297.1 2529272..2529886(-) (comEA) [Bacillus velezensis strain L-H15]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363