Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AOT11_RS09840 Genome accession   NZ_CP012881
Coordinates   2088249..2088698 (-) Length   149 a.a.
NCBI ID   WP_176309687.1    Uniprot ID   -
Organism   Vibrio vulnificus NBRC 15645 = ATCC 27562     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2083249..2093698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT11_RS09820 (AOT11_16855) coaE 2083712..2084320 (-) 609 WP_017421190.1 dephospho-CoA kinase -
  AOT11_RS09825 (AOT11_16860) pilD 2084322..2085191 (-) 870 WP_026060802.1 prepilin peptidase Machinery gene
  AOT11_RS09830 (AOT11_16865) pilC 2085285..2086511 (-) 1227 WP_017421192.1 type II secretion system F family protein Machinery gene
  AOT11_RS09835 (AOT11_16870) pilB 2086559..2088244 (-) 1686 WP_017421193.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOT11_RS09840 (AOT11_16875) pilA 2088249..2088698 (-) 450 WP_176309687.1 pilin Machinery gene
  AOT11_RS09845 (AOT11_16880) nadC 2088921..2089808 (-) 888 WP_017421195.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AOT11_RS09850 (AOT11_16885) ampD 2089901..2090473 (+) 573 WP_017421196.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AOT11_RS09855 (AOT11_16890) pdhR 2090862..2091629 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15940.41 Da        Isoelectric Point: 9.9449

>NTDB_id=137161 AOT11_RS09840 WP_176309687.1 2088249..2088698(-) (pilA) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
MKKLNKTKKQQGFTLIELMIVVAIIGVLAAIAIPAYQNYVKKSEAAIGLATAKSLITNVDMYIQEKGNFPTNDSTGFTAV
GATTTMNKLGTLSFESISGANGTIKFLFNNKSSIDTAFVSIAKTNSGWSCSFTTSNKLTNEEIPKACQP

Nucleotide


Download         Length: 450 bp        

>NTDB_id=137161 AOT11_RS09840 WP_176309687.1 2088249..2088698(-) (pilA) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
ATGAAGAAATTAAACAAAACCAAGAAACAACAAGGTTTTACCTTGATTGAGTTAATGATAGTGGTGGCAATCATTGGTGT
GTTAGCTGCAATTGCTATCCCGGCATATCAAAATTATGTCAAAAAATCTGAGGCAGCTATTGGTTTAGCTACAGCAAAAT
CACTAATAACAAACGTAGATATGTATATACAAGAAAAAGGAAACTTCCCTACGAATGATTCGACAGGCTTTACTGCGGTA
GGTGCAACAACGACAATGAATAAGTTAGGCACTTTGTCTTTCGAGAGTATAAGTGGCGCCAATGGCACGATTAAGTTTTT
ATTTAACAATAAATCTTCAATAGATACAGCCTTCGTATCAATAGCAAAAACAAATAGTGGTTGGAGCTGTAGCTTTACAA
CTAGCAATAAACTTACAAACGAAGAAATACCAAAAGCTTGTCAGCCATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)

Pilin (bacterial filament) (Pilin)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46

100

0.463
  pilA Vibrio cholerae strain A1552

46

100

0.463
  pilA Vibrio cholerae C6706

46

100

0.463
  pilA Pseudomonas aeruginosa PAK

40.909

100

0.423
  pilA Vibrio parahaemolyticus RIMD 2210633

48.837

86.577

0.423
  pilA Acinetobacter baumannii strain A118

40.972

96.644

0.396