Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AOT11_RS06240 Genome accession   NZ_CP012881
Coordinates   1349205..1350704 (-) Length   499 a.a.
NCBI ID   WP_011149205.1    Uniprot ID   -
Organism   Vibrio vulnificus NBRC 15645 = ATCC 27562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1344205..1355704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT11_RS06210 (AOT11_13280) gspK 1344802..1345824 (-) 1023 WP_026050269.1 type II secretion system minor pseudopilin GspK -
  AOT11_RS06215 (AOT11_13285) gspJ 1345817..1346518 (-) 702 WP_017420498.1 type II secretion system minor pseudopilin GspJ -
  AOT11_RS06220 (AOT11_13290) gspI 1346505..1346867 (-) 363 WP_017420499.1 type II secretion system minor pseudopilin GspI -
  AOT11_RS06225 (AOT11_13295) gspH 1346860..1347564 (-) 705 WP_017420500.1 type II secretion system minor pseudopilin GspH -
  AOT11_RS06230 (AOT11_13300) gspG 1347491..1347934 (-) 444 WP_017420501.1 type II secretion system major pseudopilin GspG -
  AOT11_RS06235 (AOT11_13305) gspF 1347988..1349205 (-) 1218 WP_017420502.1 type II secretion system inner membrane protein GspF -
  AOT11_RS06240 (AOT11_13310) pilB/pilB1 1349205..1350704 (-) 1500 WP_011149205.1 type II secretion system ATPase GspE Machinery gene
  AOT11_RS06245 (AOT11_13315) gspD 1350704..1352725 (-) 2022 WP_017420503.1 type II secretion system secretin GspD -
  AOT11_RS06250 (AOT11_13320) gspC 1352762..1353637 (-) 876 WP_157401904.1 type II secretion system protein GspC -
  AOT11_RS06255 (AOT11_13325) hslR 1354022..1354408 (+) 387 WP_011078946.1 ribosome-associated heat shock protein Hsp15 -
  AOT11_RS06260 (AOT11_13330) hslO 1354440..1355315 (+) 876 WP_017420504.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55860.17 Da        Isoelectric Point: 6.0826

>NTDB_id=137125 AOT11_RS06240 WP_011149205.1 1349205..1350704(-) (pilB/pilB1) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
MVDILDTAPSYRRLPFSFANRFKMVLEIEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=137125 AOT11_RS06240 WP_011149205.1 1349205..1350704(-) (pilB/pilB1) [Vibrio vulnificus NBRC 15645 = ATCC 27562]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AATCGAGCATCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACCTTTGTCCCTCAAGCCATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGTCAGCTAATGGAAGATATTGGCTCGGATGATGACTTCTTCTCTTTGGCCGAAGAGCTGCCGCAAGA
TGAAGATTTGCTCGAAACGGAAGACGATGCACCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GTGCGTCGGATATTCACATCGAAACGTTTGAGAAAATTTTGTCGATTCGTTTCCGTGTCGATGGCGTTTTACGCGATGTG
TTGTCTCCGAGCCGCAAACTGGCGCCGCTGTTGGTCTCTCGCGTTAAAGTCATGGCCAAACTCGACATCGCGGAAAAACG
AGTGCCACAAGATGGTCGTATTTCACTGCGTATCGGTGGTCGCGCGGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGAGAGCGTGTGGTGATGCGTTTGCTCGACAAAAACGCCACTCGTCTTGACCTGCATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGTCCTCACGGCATTATTTTGGTCACAGGTCCAACGGGTTCGGGTAAATC
CACCACCTTGTACGCTGGCTTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGACCCGATCGAATTTG
ATATTGATGGCATTGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCACGCGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAAATTCGTGACTTGGAAACCGCGCAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCCGTAGGTGCGATTACCCGTCTGCGAGACATGGGCATTGAGCCTT
TTTTGATCTCATCTTCACTGCTGGGCGTGCTGGCTCAGCGCTTGGTGCGGACCTTATGCCAAGATTGTAAAGAGCCGTAT
GAGGCCGACAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACTTTATATCGTGCCAAGGGGTGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACGGGTATCCATGAGCTGTTGATGGTGGACGATAAAGTCCAAGAGCTGA
TCCATGCAGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCGAGCATTCGCAGCGATGGTTTAGAC
AAAGTGTTGCAAGGCGTGACCTCACTGGAAGAAGTGATGCGAGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.966

100

0.453
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.51

98.998

0.421
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363