Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AO356_RS09355 Genome accession   NZ_CP012831
Coordinates   2110008..2111471 (-) Length   487 a.a.
NCBI ID   WP_060739548.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2105008..2116471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS09320 (AO356_09305) gspL 2105013..2106116 (-) 1104 WP_060739541.1 type II secretion system protein GspL -
  AO356_RS09325 (AO356_09310) - 2106116..2106961 (-) 846 WP_060739542.1 type II secretion system protein GspK -
  AO356_RS09330 (AO356_09315) - 2106958..2107491 (-) 534 WP_060739543.1 type II secretion system protein GspJ -
  AO356_RS09335 (AO356_09320) gspI 2107484..2107870 (-) 387 WP_060739544.1 type II secretion system minor pseudopilin GspI -
  AO356_RS09340 (AO356_09325) gspH 2107863..2108330 (-) 468 WP_060739545.1 type II secretion system minor pseudopilin GspH -
  AO356_RS09345 (AO356_09330) gspG 2108333..2108791 (-) 459 WP_060739546.1 type II secretion system major pseudopilin GspG -
  AO356_RS09350 (AO356_09335) gspF 2108795..2110003 (-) 1209 WP_060739547.1 type II secretion system inner membrane protein GspF -
  AO356_RS09355 (AO356_09340) pilB 2110008..2111471 (-) 1464 WP_060739548.1 type II secretion system ATPase GspE Machinery gene
  AO356_RS09360 (AO356_09345) gspD 2111468..2113393 (-) 1926 WP_060743088.1 type II secretion system secretin GspD -
  AO356_RS09365 (AO356_09350) - 2113402..2113929 (-) 528 WP_060739549.1 type II secretion system protein N -
  AO356_RS09370 (AO356_09355) - 2113926..2114468 (-) 543 WP_060739550.1 cytochrome b -
  AO356_RS09375 (AO356_09360) gbpA 2114535..2116001 (-) 1467 WP_060739551.1 N-acetylglucosamine-binding protein GbpA -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 53101.15 Da        Isoelectric Point: 6.9997

>NTDB_id=136712 AO356_RS09355 WP_060739548.1 2110008..2111471(-) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
MSEPLPFGFARRLGVLLEGAGPTLSLAMRSDTPLTAVAEVRRLCGRDLPVQVLAPAQFAARLASVYRQGQSAAEQVAQGL
DEELDLLSLADQMPQTTDLLEQQGDAPIIRLINALLSEAVREHASDVHLETFEQYLSVRMRIDGQLQEMLRPRRELANLL
VSRIKVMARLDIAEKRVPQDGRMALRLAGHEVDVRVSTLPSAHGERVVLRLLDKQAGRLDLQRLGLPDDTLASLRQLLGK
PHGIVLVTGPTGSGKTTSLYAALSSINDQSRNILTVEDPIEYHLPGIGQMPVNPKVDMTFARGLRAILRQDPDVVMVGEI
RDRETAEIAVQASLTGHLVLSTLHTNTAVGAVTRLVDMGVDAYLLASSLAGIVAQRLLRCLCPSCKAPYVADAATCRRLG
LDADAAPKLFKAQGCERCQKGYRGRFGIYELVHVNAALSQLIHQGATEQALAAEARKTSRSLFQDGRQRVLEGLTSVDEL
LRITQED

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=136712 AO356_RS09355 WP_060739548.1 2110008..2111471(-) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
GTGAGCGAGCCGTTGCCCTTTGGTTTCGCCAGGCGCCTGGGCGTGCTGCTGGAAGGCGCGGGGCCGACCTTGAGCCTGGC
GATGCGCAGCGACACGCCCCTGACTGCCGTGGCTGAAGTCCGGCGTCTGTGTGGGCGGGATCTACCTGTACAGGTCCTGG
CGCCGGCGCAGTTCGCCGCACGCCTGGCCAGCGTCTATCGCCAGGGCCAAAGCGCCGCCGAGCAGGTGGCCCAAGGCCTG
GACGAGGAACTGGACCTGCTCAGCCTGGCCGACCAGATGCCGCAGACCACCGACCTGTTGGAGCAGCAGGGTGATGCGCC
GATCATTCGCCTGATCAATGCGCTGCTCAGCGAGGCGGTCCGCGAGCATGCCTCGGATGTGCACCTGGAAACGTTCGAGC
AATACCTGTCGGTGCGCATGCGCATCGATGGGCAGTTACAGGAAATGCTGCGCCCGCGCCGGGAACTGGCGAACCTGCTG
GTGTCGCGGATCAAGGTCATGGCGCGCCTGGACATCGCCGAAAAACGCGTGCCCCAGGACGGCCGGATGGCGTTGCGCCT
GGCCGGGCACGAGGTCGATGTGCGGGTCTCGACCCTGCCTTCGGCCCATGGCGAGCGGGTGGTGTTGCGCTTGCTCGACA
AGCAGGCCGGGCGTCTGGATCTGCAACGCCTGGGCCTGCCCGACGACACCCTGGCGAGCCTGCGCCAGTTGCTCGGCAAG
CCCCATGGCATCGTGTTGGTCACCGGGCCCACGGGGTCGGGGAAGACCACCAGCCTGTACGCGGCGCTGAGCAGCATCAA
CGACCAGAGCCGCAACATCCTCACGGTCGAAGACCCCATCGAATATCACCTGCCGGGTATCGGCCAGATGCCGGTCAACC
CGAAAGTCGACATGACCTTCGCCCGCGGCTTGCGGGCCATTTTGCGCCAGGATCCGGACGTGGTGATGGTCGGCGAAATA
CGCGACCGCGAGACCGCCGAAATCGCCGTCCAGGCGTCGCTGACCGGGCACCTGGTGCTCTCGACCCTGCACACCAACAC
CGCCGTCGGCGCGGTCACGCGGCTGGTGGACATGGGCGTGGATGCGTATCTGCTGGCGTCGTCCCTGGCCGGCATCGTCG
CCCAGCGTTTGTTGCGCTGTCTGTGCCCCTCCTGCAAAGCCCCTTATGTGGCCGACGCCGCCACGTGTCGGCGCCTGGGC
CTCGACGCCGATGCCGCGCCCAAGTTGTTCAAGGCCCAGGGCTGCGAGCGATGCCAGAAAGGCTATCGCGGGCGCTTCGG
CATCTATGAGCTGGTGCATGTCAACGCCGCCCTGTCGCAGTTGATTCACCAGGGCGCCACCGAGCAGGCGCTAGCCGCCG
AGGCCCGCAAAACCTCCCGCAGCCTGTTCCAGGACGGTCGCCAGCGCGTGCTGGAAGGCCTGACCAGTGTCGACGAACTG
CTGCGCATTACCCAGGAGGATTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-379)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

43.841

98.357

0.431
  pilB Vibrio cholerae strain A1552

44.111

95.893

0.423
  pilB/pilB1 Synechocystis sp. PCC 6803

38.667

100

0.417
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.141

100

0.415
  pilB Acinetobacter baumannii D1279779

45.667

87.68

0.4
  pilB Legionella pneumophila strain ERS1305867

41.015

97.125

0.398
  pilF Thermus thermophilus HB27

39.069

100

0.396
  pilB Vibrio parahaemolyticus RIMD 2210633

46.985

81.725

0.384
  pilB Acinetobacter baylyi ADP1

45.06

85.216

0.384
  pilF Neisseria gonorrhoeae MS11

46.447

80.903

0.376