Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FORC17_RS14400 Genome accession   NZ_CP012739
Coordinates   3101843..3103342 (-) Length   499 a.a.
NCBI ID   WP_011078943.1    Uniprot ID   A0A3Q0L2V2
Organism   Vibrio vulnificus strain FORC_017     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3096843..3108342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC17_RS14370 (FORC17_2811) gspK 3097440..3098462 (-) 1023 WP_047110366.1 type II secretion system minor pseudopilin GspK -
  FORC17_RS14375 (FORC17_2812) gspJ 3098455..3099156 (-) 702 WP_065090793.1 type II secretion system minor pseudopilin GspJ -
  FORC17_RS14380 (FORC17_2813) gspI 3099143..3099505 (-) 363 WP_065090794.1 type II secretion system minor pseudopilin GspI -
  FORC17_RS14385 (FORC17_2814) gspH 3099498..3100202 (-) 705 WP_065090795.1 type II secretion system minor pseudopilin GspH -
  FORC17_RS14390 (FORC17_2815) gspG 3100129..3100572 (-) 444 WP_017420501.1 type II secretion system major pseudopilin GspG -
  FORC17_RS14395 (FORC17_2816) gspF 3100626..3101843 (-) 1218 WP_011078942.1 type II secretion system inner membrane protein GspF -
  FORC17_RS14400 (FORC17_2817) pilB/pilB1 3101843..3103342 (-) 1500 WP_011078943.1 type II secretion system ATPase GspE Machinery gene
  FORC17_RS14405 (FORC17_2818) gspD 3103342..3105363 (-) 2022 WP_011078944.1 type II secretion system secretin GspD -
  FORC17_RS14410 (FORC17_2819) gspC 3105400..3106275 (-) 876 WP_157401904.1 type II secretion system protein GspC -
  FORC17_RS14415 (FORC17_2820) hslR 3106660..3107046 (+) 387 WP_011078946.1 ribosome-associated heat shock protein Hsp15 -
  FORC17_RS14420 (FORC17_2821) hslO 3107078..3107953 (+) 876 WP_011078947.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55846.14 Da        Isoelectric Point: 6.0826

>NTDB_id=136290 FORC17_RS14400 WP_011078943.1 3101843..3103342(-) (pilB/pilB1) [Vibrio vulnificus strain FORC_017]
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=136290 FORC17_RS14400 WP_011078943.1 3101843..3103342(-) (pilB/pilB1) [Vibrio vulnificus strain FORC_017]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AGTCGAGCATCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTCCGTCGTG
TGCTCAAACAGACGTTTGTCCCTCAAGCCATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGCCAGTTGATGGAAGACATTGGCTCGGATGATGACTTCTTCTCTTTGGCTGAAGAGTTGCCGCAAGA
TGAAGATTTGCTCGAAACGGAAGACGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GGGCGTCAGATATTCACATCGAAACGTTCGAGAAAATTCTGTCGATTCGTTTCCGCGTCGATGGCGTTTTACGCGATGTA
CTGTCTCCGAGTCGCAAACTGGCGCCGCTGTTGGTGTCACGTGTTAAAGTTATGGCCAAACTGGATATTGCAGAAAAACG
TGTGCCACAAGATGGCCGTATTTCACTGCGTATCGGTGGTCGCGCTGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGCGAGCGTGTGGTGATGCGCTTGCTGGATAAAAACGCCACTCGTCTTGACCTGCATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGTCCTCACGGCATTATCTTGGTCACCGGTCCAACGGGTTCGGGTAAATC
CACCACCTTGTACGCTGGCCTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGATCCGATCGAATTTG
ATATCGATGGTATCGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCTCGTGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTTGGGGAAATTCGTGACTTGGAAACCGCGCAAATTGCTGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACTTTGCACACCAACACCGCCGTAGGTGCCATTACCCGTCTGCGTGACATGGGTATTGAGCCCT
TTTTGATCTCTTCTTCACTGCTGGGCGTGCTGGCTCAGCGCCTGGTACGGACTTTATGCCAAGACTGTAAAGAACCGTAC
GAGGCTGACAAAGAACAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACTTTATATCGTGCCAAGGGATGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACGGGTATCCATGAGCTGTTGATGGTGGACGATAAAGTCCAAGAGCTGA
TCCATGCTGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCGAGCATTCGCAGCGATGGTTTAGAC
AAAGTGTTGCAAGGCGTGACCTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L2V2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.966

100

0.453
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.713

98.998

0.423
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363

Multiple sequence alignment