Detailed information NTG0133724

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QI18_RS09110
• Genome accession: NZ_CP010050
• Coordinates: 1852217..1852864 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1847217..1857864

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QI18_RS09085 (QI18_09085)	atpF	1847286..1847792 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
QI18_RS09090 (QI18_09090)	atpB	1847807..1848520 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
QI18_RS09095 (QI18_09095)	-	1848565..1848780 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
QI18_RS09100 (QI18_09100)	-	1848965..1849741 (-)	777	WP_039115826.1	alpha/beta hydrolase	-
QI18_RS09105 (QI18_09105)	comEC	1850026..1852236 (-)	2211	WP_039115828.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QI18_RS09110 (QI18_09110)	comEA	1852217..1852864 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
QI18_RS09115 (QI18_09115)	-	1852924..1854285 (-)	1362	WP_039115830.1	ABC transporter permease	-
QI18_RS09120 (QI18_09120)	-	1854282..1855214 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
QI18_RS09125 (QI18_09125)	-	1855319..1855717 (-)	399	WP_023188528.1	hypothetical protein	-
QI18_RS09130 (QI18_09130)	-	1855821..1856384 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
QI18_RS09135 (QI18_09135)	-	1856557..1857735 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=133724 QI18_RS09110 WP_010906131.1 1852217..1852864(-) (comEA) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=133724 QI18_RS09110 WP_010906131.1 1852217..1852864(-) (comEA) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386