Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   HKU360_RS00650 Genome accession   NZ_CP009612
Coordinates   102691..103725 (+) Length   344 a.a.
NCBI ID   WP_223845212.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain HKU360     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97691..108725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKU360_RS00640 (HKU360_00132) - 101359..101724 (+) 366 WP_002986560.1 DUF1033 family protein -
  HKU360_RS00645 (HKU360_00133) comYA 101817..102755 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  HKU360_RS00650 (HKU360_00134) comYB 102691..103725 (+) 1035 WP_223845212.1 competence type IV pilus assembly protein ComGB Machinery gene
  HKU360_RS00655 (HKU360_00135) comYC 103727..104053 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  HKU360_RS00660 (HKU360_00136) comGD 104028..104456 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  HKU360_RS00665 (HKU360_00137) comGE 104413..104697 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  HKU360_RS00670 (HKU360_00138) comYF 104690..105124 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  HKU360_RS00675 (HKU360_00139) comGG 105108..105434 (+) 327 WP_002987787.1 competence type IV pilus minor pilin ComGG -
  HKU360_RS00680 (HKU360_00140) comYH 105532..106485 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  HKU360_RS00685 (HKU360_00141) - 106544..107740 (+) 1197 WP_002987791.1 acetate kinase -
  HKU360_RS00690 - 107927..108235 (+) 309 WP_002987795.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39734.23 Da        Isoelectric Point: 9.7085

>NTDB_id=130711 HKU360_RS00650 WP_223845212.1 102691..103725(+) (comYB) [Streptococcus pyogenes strain HKU360]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWSTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGVELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=130711 HKU360_RS00650 WP_223845212.1 102691..103725(+) (comYB) [Streptococcus pyogenes strain HKU360]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGAGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGTTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.462

94.477

0.401

Multiple sequence alignment