Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   lpt_RS06275 Genome accession   NZ_CP012019
Coordinates   1407544..1409028 (-) Length   494 a.a.
NCBI ID   WP_010947050.1    Uniprot ID   Q5ZVW9
Organism   Legionella pneumophila subsp. pneumophila strain Toronto-20052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1402544..1414028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  lpt_RS06260 (lpt_06250) cysS 1403546..1404916 (+) 1371 WP_061784246.1 cysteine--tRNA ligase -
  lpt_RS06265 (lpt_06255) - 1405086..1406999 (+) 1914 WP_061784247.1 DUF3413 domain-containing protein -
  lpt_RS06270 (lpt_06260) - 1407129..1407443 (+) 315 WP_061476081.1 DUF6482 family protein -
  lpt_RS06275 (lpt_06265) pilB 1407544..1409028 (-) 1485 WP_010947050.1 GspE family T2SS ATPase variant LspE Machinery gene
  lpt_RS06280 (lpt_06270) lspD 1409032..1411407 (-) 2376 WP_011213622.1 GspD family T2SS secretin variant LspD -
  lpt_RS06285 (lpt_06275) - 1411580..1412608 (+) 1029 WP_061784248.1 Gfo/Idh/MocA family protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 55151.48 Da        Isoelectric Point: 5.4872

>NTDB_id=130714 lpt_RS06275 WP_010947050.1 1407544..1409028(-) (pilB) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=130714 lpt_RS06275 WP_010947050.1 1407544..1409028(-) (pilB) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACAGTTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATACAAGA
AAATCTGGCTATTGTCTATCACTTACCTAACACACCACTGCAAGCTTTCGCTGAGATAAAGCGATTATTACAATGTGAAC
TTGATCTAAAACAAGTCGATGAATCCACTTTTCAGCAACATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCTGCAGAAGGCATGGAAGAAGATATGGATTTATCCATGTTGGCCAGTCAACTTCCCGTCAGTGAAGACTTATTGGAAAA
CCAAGACGATGCCCCAATTATCCGCTTACTGAACGCTCTGTTTACCCAAGCGATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTGGTGCGCAATCGAATTGATGGTGTTCTACAAGAGGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGAATTCCTCAAGATGGTCG
AATTTCATTGCGTATAGGAGGACATAATATTGATGTCAGGGTCTCAACTTTACCCTCAAACCATGGAGAACGAGTAGTTT
TAAGGATATTAGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTGAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCCCATGGCATTATTTTAGTCACTGGCCCAACAGGATCAGGCAAGACAACTTCTCTCTATGCTAT
GCTCACCGAACTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGACCTGCCTGGTGTAGGAC
AAACGCAGGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCCATATTACGTCAAGACCCGGATGTTGTC
ATGATAGGAGAAATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCAAGCTTAACAGGGCATTTGGTGCTATCAAC
CCTTCATACCAATACCGCATTGGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGCCTCATTGCGCAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCTCATGAATTAAGGCAGGAAGAA
AAGGAGTTAATGGGTCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAGCCCAAGGGATGCGACTATTGCAACCACTT
GGGTTATAAAGGCAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACAATGGAAAATTATTTACGACCAACCACGCCGAGCATACGTGAAGATGGTTTTAAAAGAGTATTATGTGGC
GATACGTCACTTGCAGAAATTTTAAGGGTAACATCCCAAAATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(119-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZVW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.067

96.356

0.415
  pilB Vibrio campbellii strain DS40M4

46.305

82.186

0.381
  pilB Acinetobacter baumannii D1279779

44.952

84.211

0.379
  pilB Vibrio parahaemolyticus RIMD 2210633

46.04

81.781

0.377
  pilB Acinetobacter baylyi ADP1

47.558

78.745

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

41.475

87.854

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.524

79.15

0.36

Multiple sequence alignment