Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PA50071_RS22165 Genome accession   NZ_CP012001
Coordinates   4806626..4808035 (-) Length   469 a.a.
NCBI ID   WP_003120811.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4801626..4813035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA50071_RS22150 (PA50071_22165) - 4802594..4803706 (-) 1113 WP_003112720.1 substrate-binding domain-containing protein -
  PA50071_RS22155 (PA50071_22170) lapA 4803792..4804898 (-) 1107 WP_003112123.1 alkaline phosphatase LapA -
  PA50071_RS22160 (PA50071_22175) pilC 4805412..4806626 (-) 1215 WP_024928895.1 type II secretion system inner membrane protein GspF Machinery gene
  PA50071_RS22165 (PA50071_22180) pilB 4806626..4808035 (-) 1410 WP_003120811.1 type II secretion system ATPase GspE Machinery gene
  PA50071_RS22170 (PA50071_22185) gspD 4808032..4810443 (-) 2412 WP_023115013.1 type II secretion system secretin GspD -
  PA50071_RS22175 (PA50071_22190) - 4810440..4811033 (-) 594 WP_003112724.1 type II secretion system protein M -
  PA50071_RS22180 (PA50071_22195) gspL 4811020..4812231 (-) 1212 WP_016253830.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50994.41 Da        Isoelectric Point: 5.7943

>NTDB_id=130063 PA50071_RS22165 WP_003120811.1 4806626..4808035(-) (pilB) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHALASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPACNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=130063 PA50071_RS22165 WP_003120811.1 4806626..4808035(-) (pilB) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGCACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCTGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTTACCGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCTGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTATCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTGGACAAGCAGGCCG
GACGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCTTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCGGTGACGCGGCTGGTGGACATGGGGGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTCGGCTGCCCGGCTTGCAACCAG
ACCGGCTACAGCGGGCGCACCGGCATCCACGAACTGTTTGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCGGCTGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373