Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACG06_RS09415 Genome accession   NZ_CP011857
Coordinates   1977736..1979244 (+) Length   502 a.a.
NCBI ID   WP_003158838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ATCC 27853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1972736..1984244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG06_RS09395 (ACG06_09410) - 1972767..1973978 (+) 1212 WP_003110062.1 O-succinylhomoserine sulfhydrylase -
  ACG06_RS09400 (ACG06_09415) - 1973975..1974742 (+) 768 WP_003103539.1 SDR family oxidoreductase -
  ACG06_RS09405 (ACG06_09420) xcpQ 1974828..1976804 (-) 1977 WP_003103536.1 GspD family T2SS secretin variant XcpQ -
  ACG06_RS09410 (ACG06_09425) xcpP 1976809..1977516 (-) 708 WP_003119750.1 type II secretion system protein N -
  ACG06_RS09415 (ACG06_09430) pilB 1977736..1979244 (+) 1509 WP_003158838.1 GspE family T2SS ATPase variant XcpR Machinery gene
  ACG06_RS09420 (ACG06_09435) xcpS 1979244..1980461 (+) 1218 WP_003091376.1 GspF family T2SS innner membrane protein variant XcpS -
  ACG06_RS09425 (ACG06_09440) xcpT 1980466..1980912 (+) 447 WP_003119751.1 GspG family T2SS major pseudopilin variant XcpT -
  ACG06_RS09430 (ACG06_09445) xcpU 1980919..1981437 (+) 519 WP_003103530.1 GspH family T2SS minor pseudopilin variant XcpU -
  ACG06_RS09435 (ACG06_09450) xcpV 1981434..1981823 (+) 390 WP_003103528.1 GspI family T2SS minor pseudopilin variant XcpV -
  ACG06_RS09440 (ACG06_09455) xcpW 1981820..1982533 (+) 714 WP_003110067.1 GspJ family T2SS minor pseudopilin variant XcpW -
  ACG06_RS09445 (ACG06_09460) xcpX 1982530..1983531 (+) 1002 WP_016852649.1 GspK family T2SS minor pseudopilin variant XcpX -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55552.74 Da        Isoelectric Point: 6.2074

>NTDB_id=129408 ACG06_RS09415 WP_003158838.1 1977736..1979244(+) (pilB) [Pseudomonas aeruginosa strain ATCC 27853]
MMTAPLPDIAAPAAPPRRLPFSFAKRQGLLFLCLEEQYWLACRPQVELAAIAEAQRFAGRRLPLRALGEDAFNQALAASY
QHDSSAAMQLAEDLGGSLDLAALADQVPETEDLMEQEDDAPIIRLINAILGEAIRENASDIHLETFEKRLVVRFRVDGVL
REVLEPKRELAALLVSRIKVMARLDIAEKRIPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLNLQHLGM
SERDRKLMDETVRKPHGILLVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYHLEGIGQTQVNAKVDMTFARGLRA
ILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAIGAITRLVDMGIEPFLLSSSMLGVLAQRLVRVLCPACK
EPYRADEAECALLGVDPAAPPTLHRARGCGECHQHGYRGRTGIYELVVFDDHMRSLIHNESSEQEMTRHARTSGPSIRDD
GRRKVLEGVTTVEEVLRVTREE

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=129408 ACG06_RS09415 WP_003158838.1 1977736..1979244(+) (pilB) [Pseudomonas aeruginosa strain ATCC 27853]
ATGATGACAGCCCCATTACCCGACATCGCCGCGCCGGCCGCACCGCCACGGCGTCTGCCGTTCAGCTTCGCCAAGCGCCA
GGGCCTGTTGTTCCTGTGCCTGGAGGAGCAGTACTGGCTGGCCTGCCGGCCGCAGGTGGAGCTGGCGGCGATCGCCGAGG
CGCAGCGCTTCGCCGGCCGCCGCCTGCCGCTCAGGGCGCTCGGCGAGGACGCCTTCAACCAGGCCCTGGCGGCCTCCTAC
CAGCACGACTCCTCGGCGGCCATGCAGCTCGCCGAGGACCTCGGCGGCAGCCTGGACCTCGCCGCGCTGGCCGACCAGGT
CCCGGAAACCGAGGACCTGATGGAGCAGGAGGACGACGCGCCGATCATTCGCCTGATCAACGCGATCCTCGGCGAGGCGA
TCCGCGAGAACGCCTCGGACATCCACCTGGAAACCTTCGAGAAGCGCCTGGTGGTGCGCTTCCGCGTCGACGGCGTGCTG
CGCGAGGTGCTGGAGCCCAAGCGCGAGCTGGCGGCCCTGCTGGTATCGCGGATCAAGGTCATGGCGCGGCTGGACATCGC
CGAGAAGCGTATTCCCCAGGATGGGCGGATTTCCCTGCGGGTCGGCGGGCGCGAGGTGGACATCCGCGTCTCGACCCTGC
CCTCGGCCAACGGTGAACGGGTGGTGCTGCGTCTGCTCGACAAGCAGGCCGGGCGCCTGAACCTGCAGCACCTGGGCATG
AGCGAGCGCGACCGCAAGCTGATGGACGAAACGGTGCGCAAGCCGCACGGCATCCTGCTGGTCACCGGCCCCACCGGCTC
GGGCAAGACCACCACCCTGTACGCCAGCCTGACCACGCTCAACGACCGCACGCGGAACATCCTCACCGTCGAGGATCCGA
TCGAATACCACCTGGAGGGCATCGGCCAGACCCAGGTCAACGCCAAGGTCGACATGACCTTCGCCCGCGGCCTGCGGGCG
ATCCTCCGGCAGGACCCGGACGTGGTGATGGTCGGCGAGATCCGCGACCGGGAGACCGCCGAGATCGCGGTGCAGGCTTC
GCTGACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACAGCGCCATCGGCGCGATCACCCGCCTGGTGGACATGGGCA
TCGAGCCGTTCCTGCTGTCCTCCTCCATGCTTGGCGTGCTGGCCCAGCGCCTGGTGCGGGTGCTCTGCCCGGCGTGCAAG
GAGCCCTACCGCGCCGACGAGGCCGAGTGCGCGCTGCTCGGCGTCGATCCCGCCGCGCCGCCGACCCTGCATCGCGCCCG
CGGCTGCGGCGAGTGCCACCAGCATGGCTATCGCGGGCGTACCGGTATCTATGAACTGGTGGTGTTCGACGACCACATGC
GCTCGCTGATCCACAACGAATCCTCCGAGCAGGAAATGACCCGGCATGCCCGCACCAGCGGACCAAGCATCCGCGACGAC
GGTCGGCGCAAGGTGCTGGAAGGCGTCACTACGGTCGAGGAAGTCCTGCGCGTGACCCGGGAAGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.737

97.012

0.424
  pilB/pilB1 Synechocystis sp. PCC 6803

42.245

97.61

0.412
  pilB Vibrio campbellii strain DS40M4

43.949

93.825

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

47.045

84.263

0.396
  pilF Thermus thermophilus HB27

43.146

88.645

0.382
  pilB Acinetobacter baumannii D1279779

47.88

79.88

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.446

76.892

0.373
  pilF Neisseria gonorrhoeae MS11

46.954

78.486

0.369
  pilB Acinetobacter baylyi ADP1

47.68

77.291

0.369
  pilB Legionella pneumophila strain ERS1305867

47.423

77.291

0.367

Multiple sequence alignment