Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FORC16_RS00565 Genome accession   NZ_CP011775
Coordinates   120627..122126 (+) Length   499 a.a.
NCBI ID   WP_060532761.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115627..127126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC16_RS00545 (FORC16_0093) hslO 116016..116891 (-) 876 WP_011078947.1 Hsp33 family molecular chaperone HslO -
  FORC16_RS00550 (FORC16_0094) hslR 116923..117309 (-) 387 WP_011078946.1 ribosome-associated heat shock protein Hsp15 -
  FORC16_RS00555 (FORC16_0095) gspC 117694..118569 (+) 876 WP_157401904.1 type II secretion system protein GspC -
  FORC16_RS00560 (FORC16_0096) gspD 118606..120627 (+) 2022 WP_060532763.1 type II secretion system secretin GspD -
  FORC16_RS00565 (FORC16_0097) pilB/pilB1 120627..122126 (+) 1500 WP_060532761.1 type II secretion system ATPase GspE Machinery gene
  FORC16_RS00570 (FORC16_0098) gspF 122126..123343 (+) 1218 WP_011078942.1 type II secretion system inner membrane protein GspF -
  FORC16_RS00575 (FORC16_0099) gspG 123397..123840 (+) 444 WP_011078941.1 type II secretion system major pseudopilin GspG -
  FORC16_RS00580 (FORC16_0100) gspH 123767..124471 (+) 705 WP_081036266.1 type II secretion system minor pseudopilin GspH -
  FORC16_RS00585 (FORC16_0101) gspI 124464..124826 (+) 363 WP_039552224.1 type II secretion system minor pseudopilin GspI -
  FORC16_RS00590 (FORC16_0102) gspJ 124813..125514 (+) 702 WP_013572574.1 type II secretion system minor pseudopilin GspJ -
  FORC16_RS00595 (FORC16_0103) gspK 125507..126529 (+) 1023 WP_011078937.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55833.10 Da        Isoelectric Point: 5.9493

>NTDB_id=129302 FORC16_RS00565 WP_060532761.1 120627..122126(+) (pilB/pilB1) [Vibrio vulnificus strain FORC_016]
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPEAIASEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLEAEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=129302 FORC16_RS00565 WP_060532761.1 120627..122126(+) (pilB/pilB1) [Vibrio vulnificus strain FORC_016]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AGTCGAGCATCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACGTTTGTCCCTGAAGCTATTGCCTCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGCCAGTTGATGGAAGACATTGGCTCGGATGATGACTTCTTCTCTTTGGCTGAAGAGCTGCCGCAAGA
TGAAGATTTGCTGGAAGCGGAAGACGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GTGCGTCAGATATTCACATCGAAACGTTCGAGAAAATTTTGTCGATTCGTTTCCGTGTCGATGGCGTTTTGCGCGATGTG
TTGTCTCCGAGTCGCAAACTGGCGCCGCTGTTGGTCTCTCGCGTTAAAGTCATGGCCAAACTGGATATTGCAGAAAAACG
AGTGCCACAAGATGGTCGTATTTCACTGCGTATCGGTGGTCGCGCGGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGAGAGCGTGTGGTGATGCGTTTGCTGGATAAAAACGCCACTCGTCTTGACCTGCATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGTCCTCACGGCATTATCTTGGTCACAGGTCCAACCGGCTCAGGTAAATC
CACCACCTTGTACGCTGGCCTGCAAGAGCTGAACAGCAACGAGCGCAATATCTTGACGGTGGAAGACCCGATCGAATTTG
ATATTGATGGCATTGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCTCGTGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAAATTCGTGACTTGGAAACCGCACAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCAGTGGGTGCCATTACCCGTCTGCGTGACATGGGCATTGAGCCCT
TTTTGATCTCTTCTTCACTGCTGGGCGTGCTAGCTCAGCGCTTGGTGAGGACCTTATGCCAAGACTGTAAAGAACCGTAC
GAGGCAGACAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACCTTATATCGTGCCAAGGGGTGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACCGGTATCCATGAGCTGTTGATGGTAGACGATAAAGTCCAAGAGCTGA
TCCATGCTGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCAAGCATTCGCAGCGACGGTTTAGAC
AAAGTGTTGCAAGGCGTGACGTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.966

100

0.453
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.713

98.998

0.423
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363