Detailed information NTG0127714

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IX87_RS12530
• Genome accession: NZ_CP009257
• Coordinates: 2474830..2475888 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain AB30
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2469830..2480888

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IX87_RS12505 (IX87_12785)	aroK	2470105..2470674 (-)	570	WP_000643712.1	shikimate kinase AroK	-
IX87_RS12510 (IX87_12790)	pilQ	2470686..2472851 (-)	2166	WP_001017038.1	type IV pilus secretin PilQ family protein	Machinery gene
IX87_RS12515 (IX87_12795)	pilP	2472914..2473441 (-)	528	WP_000695058.1	pilus assembly protein PilP	Machinery gene
IX87_RS12520 (IX87_12800)	pilO	2473452..2474192 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
IX87_RS12525 (IX87_12805)	pilN	2474189..2474830 (-)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
IX87_RS12530 (IX87_12810)	pilM	2474830..2475888 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
IX87_RS12535 (IX87_12815)	ponA	2476050..2478605 (+)	2556	WP_000736683.1	penicillin-binding protein PBP1a	-
IX87_RS12540 (IX87_12820)	-	2478623..2479447 (-)	825	WP_086242007.1	putative RNA methyltransferase	-
IX87_RS12545 (IX87_12825)	-	2479450..2479782 (-)	333	WP_000169601.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=127714 IX87_RS12530 WP_002014066.1 2474830..2475888(-) (pilM) [Acinetobacter baumannii strain AB30]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=127714 IX87_RS12530 WP_002014066.1 2474830..2475888(-) (pilM) [Acinetobacter baumannii strain AB30]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAATGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCGGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCCTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACTATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCCTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423