Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSA3335_RS08090 Genome accession   NZ_CP008742
Coordinates   1639911..1641605 (-) Length   564 a.a.
NCBI ID   WP_031597287.1    Uniprot ID   A0A554GP76
Organism   Pseudomonas savastanoi pv. savastanoi NCPPB 3335     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1634911..1646605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSA3335_RS08070 (PSA3335_07495) yacG 1636991..1637200 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSA3335_RS08075 (PSA3335_07500) coaE 1637197..1637820 (-) 624 WP_002552021.1 dephospho-CoA kinase -
  PSA3335_RS08080 (PSA3335_07505) pilD 1637817..1638689 (-) 873 WP_002552022.1 prepilin peptidase Machinery gene
  PSA3335_RS08085 (PSA3335_07510) pilC 1638691..1639908 (-) 1218 WP_002552023.1 type II secretion system F family protein Machinery gene
  PSA3335_RS08090 (PSA3335_07515) pilB 1639911..1641605 (-) 1695 WP_031597287.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSA3335_RS08095 - 1641831..1642226 (+) 396 WP_031597289.1 pilin -
  PSA3335_RS08100 (PSA3335_07520) - 1642387..1643691 (+) 1305 WP_057425986.1 O-antigen ligase family protein -
  PSA3335_RS29950 - 1643849..1644004 (+) 156 Protein_1490 polysaccharide export protein -
  PSA3335_RS08105 (PSA3335_07525) - 1644042..1645395 (-) 1354 Protein_1491 IS3 family transposase -
  PSA3335_RS08110 - 1645517..1646125 (+) 609 Protein_1492 polysaccharide export protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62542.83 Da        Isoelectric Point: 6.1206

>NTDB_id=123984 PSA3335_RS08090 WP_031597287.1 1639911..1641605(-) (pilB) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MSDAVLTGLAKQLVLAELLTETAAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLIRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTIAIDRFFDTESGLGELADIDLGLD
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=123984 PSA3335_RS08090 WP_031597287.1 1639911..1641605(-) (pilB) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGTCTGATGCTGTCCTCACCGGTTTGGCCAAACAACTGGTCCTGGCCGAGCTGCTTACCGAAACTGCTGCGCAGCAAGC
TTATCAGCAGGCGCGCCGCGACAAGATTTCATTGGTAAGTTATCTGGTCCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCTGATCAGTTCGGCGTTCCTTTCATGGACCTTGCCAGCCTGGACAAGGAAAGCCAGCCCAAAGGCCTT
GTGAGTGAAAAGCTGATTCGTCAGCACCATGCACTGCCGCTGTGGCGTCGTGGTAATAAGCTGTTTATAGGCATCTCAGA
CCCTACCAACCACCAGGCAGTCACTGACATACAATTCAGTACCGGCCTGAACACTGAAGCGATTCTGGTTGAAGATGACA
AGCTGACCATTGCCATAGACCGTTTTTTTGACACTGAAAGCGGCCTTGGCGAGTTGGCAGACATTGATTTGGGCCTGGAC
ATCGAGCCTGCGAGTGACAAGGAAACCTCACTGGCCACTCAGAGTGATGCCGATGATGCCCCGGTCGTGCGCTTCGTTAA
CAAGATGCTGATGGACGCGATACGGCTCGGCTCGTCCGACCTGCATTTTGAACCTTACGAAAAGATCTTCCGAGTGCGGT
TGCGTACCGACGGCATTTTGCATGAGGTGGCCAGACCACCGATCCATCTGGCCAACCGTATTGCTGCGCGCCTGAAGGTC
ATGGCCAGCCTCGATATCTCTGAGCGGCGTAAACCGCAGGACGGCCGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCTAT
CGACTTCCGTATGAATACGCTGCCGACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATCCGACCAGCGCACAGA
TGGGCATTGACGCGCTGGGCTATGAGCCAGACCAAAAAGCTCTGTATATGGAGGCCTTGAAACAACCACAAGGCATGATT
CTTGTAACCGGTCCCACTGGCTCGGGTAAAACCGTTTCGCTGTACACTGGCCTGAACATTCTTAATACTGTGGACATCAA
CATTTCCACAGCTGAAGACCCGGTAGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGACTCG
ACTTTTCTCAGGCATTGCGCGCATTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATACGCGACCTGGAAACC
GCCGAAATCGCCATTAAAGCATCGCAAACTGGCCACATGGTGTTATCTACTCTGCATACTAACAGTGCCGCAGAAACCCT
CACTCGCTTGCATCATATGGGCGTCGCAGCGTTCAACATCGCTACGGCTATTAACCTCATCATTGCCCAGCGGCTGGCGC
GCAAGCTATGCAGCCATTGCAAGAAAGAGCTCGATATACCCCGGGAAACGCTCATCCAGGAGGGTTTTCCAGAGGAGAAG
ATCGGTACGTTTAAAATCTATGGCCCAGTAGGTTGTGAGCATTGCAACGGCGGTTACAGGGGCAGGGTTGGTATTTACGA
AGTAGTAAAGAAAACACCAGAACTGGAACGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAATGATTTGAGGACCTCCGGGCTGTCGAAGGCCATGCAAGGTATTACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554GP76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.865

100

0.589
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.56
  pilB Vibrio cholerae strain A1552

50.98

99.468

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.722

98.227

0.498
  pilB Vibrio campbellii strain DS40M4

49.468

100

0.495
  pilF Neisseria gonorrhoeae MS11

48.043

99.645

0.479
  pilF Thermus thermophilus HB27

39.223

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.279

93.44

0.367

Multiple sequence alignment