Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   B224_RS10725 Genome accession   NZ_CP007567
Coordinates   2388123..2389262 (+) Length   379 a.a.
NCBI ID   WP_041205991.1    Uniprot ID   -
Organism   Aeromonas media WS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2383123..2394262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B224_RS10695 (B224_3020) - 2383210..2383590 (-) 381 WP_005328065.1 diacylglycerol kinase -
  B224_RS10700 (B224_3021) - 2383731..2384795 (+) 1065 WP_005328062.1 3-deoxy-7-phosphoheptulonate synthase -
  B224_RS10705 (B224_3022) - 2385003..2385788 (-) 786 WP_005328061.1 ATP-binding cassette domain-containing protein -
  B224_RS10710 (B224_3024) btuC 2385775..2386782 (-) 1008 WP_005328058.1 vitamin B12 ABC transporter permease BtuC -
  B224_RS10715 (B224_3026) - 2386868..2387599 (-) 732 WP_041205990.1 substrate-binding periplasmic protein -
  B224_RS10720 (B224_3028) - 2387712..2388068 (+) 357 WP_005328056.1 DMT family protein -
  B224_RS10725 (B224_3029) pilU 2388123..2389262 (+) 1140 WP_041205991.1 type IVa pilus ATPase TapW Machinery gene
  B224_RS10730 (B224_3031) glgC 2389534..2390805 (+) 1272 WP_041205992.1 glucose-1-phosphate adenylyltransferase -
  B224_RS10735 (B224_3033) - 2390957..2391199 (-) 243 WP_005328051.1 FeoC-like transcriptional regulator -
  B224_RS10740 (B224_3036) feoB 2391243..2393513 (-) 2271 WP_005328050.1 Fe(2+) transporter permease subunit FeoB -
  B224_RS10745 (B224_3035) - 2393510..2393737 (-) 228 WP_005328049.1 FeoA family protein -
  B224_RS10750 (B224_3037) - 2393858..2394187 (+) 330 WP_005328048.1 translation initiation factor -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42402.45 Da        Isoelectric Point: 6.4288

>NTDB_id=121012 B224_RS10725 WP_041205991.1 2388123..2389262(+) (pilU) [Aeromonas media WS]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLNEMPLEPGEVARIAADLMDSEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTEGGRRAAVEVMIGTHTIRDLIKRGEFGGLKDIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWKEKGQIGNSAEASGWSLEPSKDENNDIF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=121012 B224_RS10725 WP_041205991.1 2388123..2389262(+) (pilU) [Aeromonas media WS]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTCTCCACCGGGGCCCCCCCCTG
CGCCAAGTTCAATGGCGCCCTGCGCCCCTTGAACGAGATGCCGCTCGAGCCGGGCGAAGTGGCCAGGATCGCCGCTGATC
TCATGGACTCGGAGCAGAAGCAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCTCTGGTGGCGCGCAACATCAAGACCGAGATACCCCGTTTCGA
GGATCTCAAACTCCCCCCGGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGTGGTCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGACACCCGCAGCTTCCATGC
GGCCCTGAAGAACACCCTGCGCCAGGCACCGGACGTCATCCTCATCGGTGAGATCCGTGACCGGGAGACCATGGAGCATG
CGCTGGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCAGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCACCGAGGGCGGCCGCCGGGCGGCGGTGGAGGTCATGATCGGCACTCACACCATTCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGATATCATGGAGAAATCCAGGGCCCTTGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTGGTGGTGGAGGGGGTGATCGACGAAGAGGAGGCAGTCAAGAACGCCGATTCCCAGAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAAAAGGGGCAGATAGGCAACAGTGCGGAGGCCAGCGGCTGGAGCCTGGAGCCCAGCAAGGATG
AGAACAACGATATCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.667

98.945

0.541
  pilU Acinetobacter baylyi ADP1

52.394

93.668

0.491
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.305

98.681

0.388
  pilT Pseudomonas aeruginosa PAK

41.57

90.765

0.377
  pilT Legionella pneumophila strain ERS1305867

42.515

88.127

0.375
  pilT Legionella pneumophila strain Lp02

42.515

88.127

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.171

92.612

0.372
  pilT Vibrio cholerae strain A1552

40.171

92.612

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.988

90.765

0.372
  pilT Acinetobacter baumannii D1279779

41.916

88.127

0.369
  pilT Acinetobacter baumannii strain A118

41.916

88.127

0.369
  pilT Acinetobacter nosocomialis M2

41.916

88.127

0.369

Multiple sequence alignment