Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B224_RS00445 Genome accession   NZ_CP007567
Coordinates   85201..86886 (-) Length   561 a.a.
NCBI ID   WP_041204906.1    Uniprot ID   -
Organism   Aeromonas media WS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 87386..88522 85201..86886 flank 500


Gene organization within MGE regions


Location: 85201..88522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B224_RS00445 (B224_0113) pilB 85201..86886 (-) 1686 WP_041204906.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  B224_RS23890 - 86927..87340 (-) 414 WP_081805615.1 hypothetical protein -
  B224_RS00455 (B224_0114) - 87386..88522 (-) 1137 WP_041204891.1 ISAs1-like element ISAeme1 family transposase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61972.20 Da        Isoelectric Point: 5.6694

>NTDB_id=121002 B224_RS00445 WP_041204906.1 85201..86886(-) (pilB) [Aeromonas media WS]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLMESDQDTLGMDHIDEAEISELEVSD
ESSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAARLKVMARL
DIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSASRLNLDQLGFDERQKAQYLHALSKPQGMILVTG
PTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRDLETAEIA
IKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFTQQQLAAG
LRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLAEINRITT
N

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=121002 B224_RS00445 WP_041204906.1 85201..86886(-) (pilB) [Aeromonas media WS]
ATGACATCTAGCCTCAATAGCGGCCTGGCCACCAGCTTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAACGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAACCCTTTGTCACCTTCCTGATCGAAAATGACATCCTCGACAGCAAGGCCCTCG
CGGATTTCTGCGAGCTGGAGTATGGAGTACCTCTGCTGGATCTTGCCGCCTTCGACCTCTCCGAAATCCCGCAGAAGTAC
CTCAATCAGAAATTGATCGAAAAGCACCACGTCCTGCCCATCTACACCCAAGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTATCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACCGAGGCCTTGCTGGTTGAAGAGGGCA
AGCTGATGGAAAGCGATCAGGATACCCTGGGCATGGATCACATCGACGAGGCCGAAATCTCGGAACTCGAGGTCTCCGAT
GAGAGTTCGCGACTCGACGACAGCGTCAACACTGCCGACGACGATGCCCCCATCGTCAAATACATTCACAAGATCATGAT
GGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACCGGATCCGCTTCCGGATCGACG
GCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGCTTAAAGGTAATGGCGCGGCTA
GATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAACCGGTCCATGGATATGCGGGT
CAACACCCTGCCCACCATGTGGGGAGAAAAGGTTGTCATTCGCCTGCTGGACTCGTCAGCCTCTCGCCTCAACCTCGATC
AACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAACCACAGGGCATGATACTGGTCACGGGC
CCCACGGGATCGGGCAAGACGGTGTCACTCTATACCGGTCTCAACATCCTCAATACCAGCGAGGTCAACATCTCCACCGC
TGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGATCAATCCCAAGGCGGGGCTCACCTTCGCCAGCG
CCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGATCTGGAAACCGCCGAGATTGCC
ATCAAGGCCGCTCAGACCGGACACCTGGTACTGTCAACTCTGCACACCAACTCGGCGGCCGAGACCCTGACCCGGATGAT
GAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGCGCCTCGCCCGCAAGCTGTGCG
ATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACCCAGCAACAGCTGGCCGCCGGT
TTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGTCGGCATCTATGAAATCATGCT
GATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTGCCATCGCCCAGAAAGAGGGTA
TGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCCGAGATCAACAGGATCACGACC
AACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.342

100

0.563
  pilB Acinetobacter baylyi ADP1

54.529

100

0.547
  pilB Legionella pneumophila strain ERS1305867

53.723

100

0.54
  pilB Vibrio cholerae strain A1552

52.313

100

0.524
  pilB Vibrio parahaemolyticus RIMD 2210633

48.845

100

0.49
  pilB Vibrio campbellii strain DS40M4

48.49

100

0.487
  pilF Neisseria gonorrhoeae MS11

46.786

99.822

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

38.156

98.574

0.376
  pilF Thermus thermophilus HB27

39.539

92.87

0.367

Multiple sequence alignment