Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RR32_RS14975 Genome accession   NZ_CP010368
Coordinates   3179316..3180806 (-) Length   496 a.a.
NCBI ID   WP_032048516.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain 6411     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3174316..3185806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RR32_RS14945 (RR32_14950) gigA 3175309..3176277 (+) 969 WP_002054548.1 RsbU family protein phosphatase GigA -
  RR32_RS14950 (RR32_14955) gigB 3176380..3176901 (+) 522 WP_000103065.1 anti-anti-sigma factor GigB -
  RR32_RS14955 (RR32_14960) - 3176928..3177752 (+) 825 WP_032048519.1 carbon-nitrogen hydrolase family protein -
  RR32_RS14960 (RR32_14965) - 3177767..3178144 (+) 378 WP_032048518.1 DUF4951 domain-containing protein -
  RR32_RS14965 (RR32_14970) - 3178304..3178741 (+) 438 WP_004710519.1 MarR family winged helix-turn-helix transcriptional regulator -
  RR32_RS14970 (RR32_14975) - 3178829..3179260 (+) 432 WP_000051124.1 organic hydroperoxide resistance protein -
  RR32_RS14975 (RR32_14980) pilB 3179316..3180806 (-) 1491 WP_032048516.1 type II secretion system ATPase GspE Machinery gene
  RR32_RS14980 (RR32_14985) - 3180903..3181724 (+) 822 WP_002054549.1 hypothetical protein -
  RR32_RS14985 (RR32_14990) - 3181734..3182651 (+) 918 WP_004883114.1 mechanosensitive ion channel family protein -
  RR32_RS14990 (RR32_14995) - 3182641..3185448 (-) 2808 WP_032048515.1 DUF2339 domain-containing protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55343.25 Da        Isoelectric Point: 6.2040

>NTDB_id=120258 RR32_RS14975 WP_032048516.1 3179316..3180806(-) (pilB) [Acinetobacter nosocomialis strain 6411]
MQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCSEQEFNSLLSTSYAGDTGESQQV
AAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQPRREL
APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQ
LVHRPHGIILVTGPTGSGKTTTLYAALSDLNDNTRNILTAEDPIEYQLEGIGQTQVNTKVDMTFARALKAMLRQDPDVVM
VGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPFLLSSSLIGVVAQRLVRTLCPHCMTWREADTFEK
QVFQHISHEPTLKLPEAQGCEQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGK
TTLEEVLRVTNEASEA

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=120258 RR32_RS14975 WP_032048516.1 3179316..3180806(-) (pilB) [Acinetobacter nosocomialis strain 6411]
ATGCAAATATTGAAACAAATACAAGTTCCATATAGTTTTGCCAAGCGTCATGGTGTTTTATTTCGTTATGACGGTGATCA
AGTTTTTATTGTTAGACGTCAAAATACAGAAAAAATTGCATTGCAGGAAGCAAGACGAATTTTGGGGAAGCCTGCACATT
ATCAGCTATGTAGTGAACAAGAGTTTAATAGCTTATTAAGTACGAGTTACGCTGGTGACACAGGGGAGTCGCAACAAGTT
GCAGCAGGTCTAGAAGATCATCCAGATTTATTGAGTTTGGCCGATCAGGTTCCTGAAACAGAAGACTTAATGGACCAAGA
AGATGACGCGCCTATTGTCCGTCTGATCAATGCACTTCTATCAGAGGCGATTCGAGTTGGTGCTTCTGATATCCATATTG
AAGCTTTTGAAAAAAAATTATCCGTTCGTTTGCGGGTAGATGGTCAATTACGTGAAATTGTACAGCCGCGCCGTGAGTTG
GCACCGTTACTTGTTTCACGTATTAAAGTTATGGCGAAACTAGATATTGCCGAAAAGCGTGTGCCGCAAGATGGACGTAT
TTCTTTACGACTTGCTGGACGTGAAGTGGATGTGCGTGTATCTACTTTGCCTTCGTCTCATGGCGAGCGAGTTGTTATGC
GTTTGCTTGATAAACAAGCGGGTCGCCTTAATATGACTCACTTGGGGTTAATGGCAAATGACTATGAGCGTTTAACTCAA
TTGGTGCATCGTCCACACGGAATTATTTTAGTTACAGGTCCTACGGGTTCGGGAAAAACCACTACCTTATATGCAGCTTT
ATCTGATTTAAATGATAATACGCGCAACATCTTAACTGCCGAAGACCCAATCGAATATCAACTTGAAGGCATAGGGCAAA
CACAAGTTAATACCAAAGTGGATATGACATTCGCTCGTGCTTTAAAGGCAATGTTACGTCAAGATCCAGATGTTGTAATG
GTTGGTGAGATTCGTGATTTAGAAACTGCTGAAATTGCAGTGCAAGCATCATTAACAGGTCATTTGGTTTTATCGACACT
ACATACCAATACTGCTATTGGTGCGGTTACTCGTCTTAAAGATATGGGAATTGAACCGTTCTTACTTTCAAGTTCTTTAA
TTGGGGTCGTTGCTCAGCGTTTGGTTCGTACCTTATGTCCACATTGTATGACATGGCGTGAAGCCGATACTTTTGAAAAG
CAGGTTTTTCAGCATATTTCGCATGAGCCAACTTTAAAGTTACCGGAAGCTCAAGGGTGTGAGCAATGTTCCCACTTAGG
GTTTAATGGCCGTACAGCGATTTATGAAATTGTACCTATTGATGAACCTATGCGCCGACTCATTCATGGTAATGCAGCAG
AGTTTGAATTAGAAAATCATGCCCGTCAGTATTCTGGTTCTATTCGAGATGATGGATTGCGTAAAGTGCTCTCAGGCAAA
ACTACACTTGAAGAAGTACTTCGTGTAACTAATGAAGCAAGTGAAGCATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(118-384)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

41.803

98.387

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.057

99.194

0.407
  pilB Vibrio campbellii strain DS40M4

48.622

80.444

0.391
  pilF Thermus thermophilus HB27

39.351

99.395

0.391
  pilB Vibrio parahaemolyticus RIMD 2210633

48.25

80.645

0.389
  pilB Vibrio cholerae strain A1552

48

80.645

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

39.834

97.177

0.387
  pilF Neisseria gonorrhoeae MS11

48.223

79.435

0.383
  pilB Acinetobacter baumannii D1279779

47.146

81.25

0.383
  pilB Acinetobacter baylyi ADP1

46.787

78.427

0.367

Multiple sequence alignment