Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NTHI723_RS06245 Genome accession   NZ_CP007472
Coordinates   1261178..1262515 (+) Length   445 a.a.
NCBI ID   WP_011272046.1    Uniprot ID   Q4QNB0
Organism   Haemophilus influenzae strain 723     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1256178..1267515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI723_RS06220 (NTHI723_01249) - 1256243..1258837 (-) 2595 WP_044364772.1 penicillin-binding protein 1A -
  NTHI723_RS06225 (NTHI723_01250) comA 1258936..1259733 (+) 798 WP_011272050.1 pilus assembly protein PilM Machinery gene
  NTHI723_RS06230 (NTHI723_01251) comB 1259734..1260240 (+) 507 WP_011272049.1 competence protein ComB Machinery gene
  NTHI723_RS06235 (NTHI723_01252) comC 1260237..1260758 (+) 522 WP_011272048.1 competence protein ComC Machinery gene
  NTHI723_RS06240 (NTHI723_01253) comD 1260755..1261168 (+) 414 WP_011272047.1 pilus assembly protein PilP Machinery gene
  NTHI723_RS06245 (NTHI723_01254) comE 1261178..1262515 (+) 1338 WP_011272046.1 type IV pilus secretin PilQ Machinery gene
  NTHI723_RS06250 (NTHI723_01255) - 1262528..1263214 (+) 687 WP_011272045.1 ComF family protein -
  NTHI723_RS06255 (NTHI723_01256) nfuA 1263290..1263886 (+) 597 WP_005649300.1 Fe-S biogenesis protein NfuA -
  NTHI723_RS06260 (NTHI723_01257) nudC 1263953..1264747 (+) 795 WP_005686893.1 NAD(+) diphosphatase -
  NTHI723_RS06265 (NTHI723_01258) - 1264783..1265373 (+) 591 WP_005686892.1 YjaG family protein -
  NTHI723_RS06270 (NTHI723_01259) - 1265513..1265785 (+) 273 WP_005630954.1 HU family DNA-binding protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49137.44 Da        Isoelectric Point: 7.7981

>NTDB_id=119777 NTHI723_RS06245 WP_011272046.1 1261178..1262515(+) (comE) [Haemophilus influenzae strain 723]
MKKYFLKCGYFLVCFCLPLIVFANPKTDNECFFIRLSQAPLAQTLEQLAFQQDVNLVMGERLEGNISLKLNNIDMPRLLK
IIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSN
LLVIQDEPRFVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLEALKQKSAGKK

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=119777 NTHI723_RS06245 WP_011272046.1 1261178..1262515(+) (comE) [Haemophilus influenzae strain 723]
ATGAAGAAATATTTTTTAAAGTGCGGTTATTTTTTAGTGTGTTTTTGTTTGCCATTAATCGTTTTTGCTAATCCTAAAAC
AGATAACGAATGTTTTTTTATTCGTTTATCGCAAGCACCTTTAGCTCAAACACTGGAGCAATTAGCTTTTCAACAAGATG
TGAATTTAGTGATGGGTGAGAGGTTAGAAGGCAATATTTCTTTGAAATTAAACAATATTGATATGCCACGTTTGCTAAAA
ATAATCGCAAAAAGTAAGCATCTTACTTTGAATAAAGATGATGGGGTTTATTATTTAAACGGCAGTCAATCTGGCAAAGG
TCAAGTTGCAGGAAATCTTACGACAAATGAACCGCACTTAGTCAGCCACACGGTAAAACTTCATTTTGCTAAAGCCTCTG
AATTAATGAAATCCTTAACAACAGGAAGTGGATCTTTGCTTTCTTCTGCGGGGAGCATTACCTTTGATGATCGCAGTAAT
TTGCTGGTTATTCAGGATGAACCTCGTTTTGTGCAAAATATCAAAAAACTGATTGCTGAAATGGATAAGCCTATTGAACA
GATCGCTATTGAAGCGCGAATTGTGACAATTACGGATGAGAGTTTGAAAGAACTTGGCGTTCGGTGGGGGATTTTTAATC
CAACTGAAAATGCAAGACGAGTTGCGGGCAGCCTTACAGGCAATAGCTTTGAAAATATTGCGGATAATCTTAATGTAAAT
TTTGCGACAACGACGACACCTGCTGGCTCTATAGCATTACAAGTCGCGAAAATTAATGGGCGATTGCTTGATTTAGAATT
GAGTGCGTTGGAGCGTGAAAATAATGTAGAAATTATTGCAAGTCCTCGCTTACTCACTACCAATAAGAAAAGTGCGAGCA
TTAAACAGGGGACAGAAATTCCTTACATCGTGAGTAATACTCGTAACGATACGCAATCTGTGGAATTTCGTGAGGCAGTA
CTTGGTTTGGAAGTGACGCCACATATTTCTAAAGATAACAATATCTTACTTGATTTATTGGTAAGTCAAAATTCCCCTGG
TTCTCGTGTCGCTTATGGACAAAATGAGGTGGTTTCTATTGATAAGCAAGAAATTAATACTCAGGTTTTTGCCAAAGATG
GGGAAACCATTGTGCTTGGCGGCGTATTTCACGACACAATCACGAAAAGCGAAGATAAAGTGCCATTGCTTGGCGATATA
CCCGTTATTAAACGATTATTTAGCAAAGAAAGTGAACGACATCAAAAACGTGAGCTCGTGATTTTCGTCACGCCGCATAT
TTTAAAAGCAGGAGAAACGTTAGAGGCGTTGAAACAAAAAAGTGCGGGGAAAAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(123-187)

Bacterial type II and III secretion system protein (Secretin)

(269-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

100

100

1
  comE Haemophilus influenzae Rd KW20

97.528

100

0.975
  comE Glaesserella parasuis strain SC1401

52

95.506

0.497
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.625

100

0.409
  pilQ Vibrio cholerae strain A1552

40.625

100

0.409
  pilQ Vibrio campbellii strain DS40M4

42.959

94.157

0.404

Multiple sequence alignment