Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOQN4_RS03120 Genome accession   NZ_OZ244735
Coordinates   612210..613340 (-) Length   376 a.a.
NCBI ID   WP_153713145.1    Uniprot ID   A0A5Q2QBF8
Organism   MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 607210..618340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQN4_RS03085 - 607262..607771 (-) 510 WP_153713152.1 DUF2244 domain-containing protein -
  ACOQN4_RS03090 - 607844..608671 (-) 828 WP_170272522.1 alpha/beta fold hydrolase -
  ACOQN4_RS03095 - 608700..608867 (+) 168 WP_153713150.1 Trm112 family protein -
  ACOQN4_RS03100 sbcB 608864..610303 (-) 1440 WP_153713149.1 exodeoxyribonuclease I -
  ACOQN4_RS03105 minE 610296..610544 (-) 249 WP_153713148.1 cell division topological specificity factor MinE -
  ACOQN4_RS03110 minD 610549..611361 (-) 813 WP_153713147.1 septum site-determining protein MinD -
  ACOQN4_RS03115 minC 611439..612140 (-) 702 WP_420550930.1 septum site-determining protein MinC -
  ACOQN4_RS03120 pilU 612210..613340 (-) 1131 WP_153713145.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOQN4_RS03125 - 613497..616130 (+) 2634 WP_153713144.1 cation-translocating P-type ATPase -
  ACOQN4_RS03130 - 616163..617128 (+) 966 WP_211365835.1 LLM class oxidoreductase -
  ACOQN4_RS03135 - 617125..617895 (+) 771 WP_153713143.1 SDR family NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 40518.21 Da        Isoelectric Point: 5.4667

>NTDB_id=1171105 ACOQN4_RS03120 WP_153713145.1 612210..613340(-) (pilU) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
MTLLTWLRELDTRQGSDLYLSTAAPATVRVNGVMQPIGEPLATGAVAGLLAGVTSAAQKAEFAAKPELNFAVSEPGLGRF
RINAFKQRGEVSIVARAIKTDIPSIASLGLPPLLKQLSMAPRGLILFVGATGSGKSTSLASMVDYRNANAAGHIITIEDP
IEFVHAHKQSVVNQREVGMDTDTYQDALENTLRQAPDVILIGEIRTRETMEHAIAFAETGHLCLSTLHANNANQALDRII
NFFPEERRDQLLMDLSLNLRGIVSQRLVKTVSSARTAAVEILLGTPRVSDLIRQGEVGLLKEAMEQAGTEGMVTFDAALL
ALWKSGAVSLDEALRNADSENNLRLAINLVEQRQADGSDAGWSLTPNQEAPGQRLR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1171105 ACOQN4_RS03120 WP_153713145.1 612210..613340(-) (pilU) [MAG: Litorivicinus lipolyticus isolate cfb32c0c-0c5a-4fc9-8b22-2b72eeb7acf8]
ATGACATTACTGACTTGGTTACGTGAACTGGACACCCGTCAGGGTTCGGACCTTTACCTGTCGACGGCAGCACCGGCGAC
CGTGCGGGTTAACGGGGTGATGCAGCCGATCGGCGAGCCGCTGGCCACTGGTGCCGTCGCGGGGCTGTTAGCCGGGGTCA
CCAGTGCGGCCCAAAAAGCCGAGTTCGCGGCCAAGCCGGAACTTAACTTCGCGGTGTCCGAGCCGGGCCTGGGGCGTTTC
CGCATCAACGCCTTTAAACAGCGCGGCGAGGTCAGCATCGTCGCCCGTGCCATCAAGACCGACATACCCAGCATTGCGTC
GTTGGGGTTGCCGCCGCTGCTAAAACAGTTATCGATGGCGCCACGCGGCCTGATCTTATTCGTTGGTGCCACCGGCAGCG
GCAAATCAACCTCGTTGGCGTCGATGGTCGACTACCGCAATGCCAATGCGGCCGGGCACATCATCACCATCGAGGATCCG
ATTGAATTTGTTCACGCGCACAAGCAATCGGTGGTCAACCAGCGCGAGGTCGGGATGGACACCGACACCTACCAGGACGC
CCTCGAAAACACCCTGCGCCAGGCCCCGGACGTCATTTTGATCGGCGAAATTCGAACCCGTGAAACCATGGAACACGCGA
TCGCCTTTGCCGAAACGGGCCACCTGTGCCTGTCGACCTTGCACGCCAATAACGCCAATCAAGCCTTGGATCGCATTATT
AACTTTTTCCCCGAGGAGCGACGCGACCAGTTGCTGATGGACCTGTCATTGAACTTGCGCGGGATTGTGTCCCAGCGTTT
GGTTAAAACGGTTAGCAGTGCGCGCACCGCCGCGGTCGAAATTCTATTGGGCACACCCCGGGTCAGCGACTTGATACGTC
AGGGCGAGGTGGGACTGCTCAAAGAAGCCATGGAGCAGGCCGGAACCGAGGGCATGGTGACCTTTGATGCGGCGCTGTTG
GCGTTATGGAAATCAGGGGCCGTTAGCCTGGACGAGGCGCTGCGCAATGCGGACAGTGAAAACAACCTGCGTTTGGCCAT
TAATCTGGTCGAGCAGCGCCAAGCGGACGGCAGCGATGCCGGTTGGTCATTGACGCCAAACCAAGAGGCCCCGGGCCAGC
GGTTGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q2QBF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.219

100

0.532
  pilU Vibrio cholerae strain A1552

51.576

92.819

0.479
  pilU Acinetobacter baylyi ADP1

52.187

91.223

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.424

96.543

0.41
  pilT Pseudomonas aeruginosa PAK

40.643

90.957

0.37
  pilT Pseudomonas stutzeri DSM 10701

40.058

90.957

0.364

Multiple sequence alignment