Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOFKU_RS15435 Genome accession   NZ_OZ241361
Coordinates   3311984..3313681 (-) Length   565 a.a.
NCBI ID   WP_419813036.1    Uniprot ID   -
Organism   MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3306984..3318681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKU_RS15410 - 3308532..3308969 (+) 438 WP_419813031.1 copper chaperone PCu(A)C -
  ACOFKU_RS15415 yacG 3309003..3309191 (-) 189 WP_419813032.1 DNA gyrase inhibitor YacG -
  ACOFKU_RS15420 coaE 3309188..3309805 (-) 618 WP_419813033.1 dephospho-CoA kinase -
  ACOFKU_RS15425 - 3309821..3310717 (-) 897 WP_419813034.1 prepilin peptidase -
  ACOFKU_RS15430 pilC 3310718..3311944 (-) 1227 WP_419813035.1 type II secretion system F family protein Machinery gene
  ACOFKU_RS15435 pilB 3311984..3313681 (-) 1698 WP_419813036.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOFKU_RS15440 - 3313793..3314287 (-) 495 WP_419813037.1 pilin -
  ACOFKU_RS15445 nadC 3314521..3315369 (-) 849 WP_419813038.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACOFKU_RS15450 - 3315509..3316885 (-) 1377 WP_419813039.1 alanine/glycine:cation symporter family protein -
  ACOFKU_RS15455 ampD 3317090..3317629 (+) 540 WP_419813040.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACOFKU_RS15460 ampE 3317626..3318501 (+) 876 WP_419813041.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 61955.76 Da        Isoelectric Point: 4.5868

>NTDB_id=1170836 ACOFKU_RS15435 WP_419813036.1 3311984..3313681(-) (pilB) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
MSVISGLPKRLVEQNHISREQALQILESAQKDKVSFVTAAVQSGQIKAQPIALLASEEFGLPLLDLSAFDFDLAPQDLVS
QDLIEKHRVLPIFTRGTRLYIAQSDPSAILAIDEIKFNTGLNIEPVLVEEDKLSNAVDKFLAANDNTFDDFDDELDDVDL
EDPEAEAKKREADQESTGDDAPIVKFINKMLLDAIKMGSSDLHFEPYEKRYRVRYRTDGVLHEVASPPVNLAGKIAARLK
VMSKMDISERRIPQDGRIKMKISKNKAIDFRVNTLPTLFGEKIVLRILDPSSAQLGIDMLGYEPEQKALYMEALGKPQGM
ILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNAKVGLDFASALRSFLRQDPDVVMVGEIRDLE
TASIAIKAAQTGHMVMSTLHTNSAPETLTRLANMGVPNFSIATSVSLIIAQRLARRLCSSCKEPADLPQEVLREAGFTDE
LLTEAEIYKPVGCDNCKEGFKGRVGIYEVVKITDPISRIIMEGGNAIEIADAANREGFNDLRTSALMKVAQGITSLEEAN
RVTTD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=1170836 ACOFKU_RS15435 WP_419813036.1 3311984..3313681(-) (pilB) [MAG: Bacterioplanoides sp. isolate a8483998-2d78-4028-8a03-01e39642ae96]
ATGAGCGTTATATCCGGCTTACCTAAACGATTAGTTGAACAAAATCACATTAGCCGGGAACAGGCACTTCAAATTCTGGA
AAGCGCCCAAAAAGACAAAGTCAGTTTTGTGACAGCGGCTGTTCAATCAGGTCAAATTAAAGCCCAACCTATTGCCCTTC
TTGCCTCCGAAGAATTCGGCCTTCCACTGCTGGACTTATCAGCTTTTGACTTTGATCTTGCACCACAAGATCTAGTGAGC
CAGGATCTTATAGAGAAACACCGAGTACTACCGATCTTTACTCGTGGAACCCGGTTATACATTGCACAAAGTGACCCTTC
TGCCATCCTGGCAATTGATGAGATCAAGTTCAATACTGGCTTAAATATAGAGCCCGTTCTTGTTGAAGAAGATAAACTGT
CTAACGCGGTCGATAAGTTCTTAGCGGCTAATGATAATACATTTGACGACTTTGATGATGAACTGGATGATGTTGACTTA
GAAGACCCGGAAGCAGAAGCGAAAAAACGCGAGGCCGACCAAGAATCCACAGGTGATGATGCACCCATTGTAAAATTCAT
CAATAAAATGCTGTTGGATGCTATCAAGATGGGGTCCTCTGACCTGCATTTTGAACCCTATGAAAAACGCTATCGGGTGC
GCTACCGCACTGATGGCGTCTTGCACGAAGTTGCCAGCCCTCCGGTGAATTTAGCGGGCAAAATTGCAGCACGCCTGAAA
GTTATGTCAAAAATGGATATTTCTGAACGCCGCATCCCACAGGATGGCCGGATTAAAATGAAAATATCCAAAAATAAGGC
CATCGACTTCCGCGTGAATACCCTTCCAACCTTGTTTGGCGAAAAAATTGTATTACGAATTCTTGACCCGTCTTCTGCCC
AATTGGGTATTGATATGCTGGGCTACGAGCCTGAACAAAAAGCTCTGTATATGGAGGCCTTAGGCAAACCACAAGGCATG
ATTCTGGTTACCGGACCAACAGGCTCAGGGAAAACTGTTTCCTTGTATACCGGCTTAAATATCCTCAATGTCGAAGGCAC
CAATATTTCTACCGCTGAAGATCCGGTCGAGATTAATCTGGAAGGTATTAATCAGGTGAACGTTAATGCCAAAGTCGGCC
TGGACTTCGCTTCAGCTTTACGCTCCTTCCTTCGACAGGATCCGGATGTGGTAATGGTCGGAGAGATCCGGGATCTGGAA
ACCGCCTCCATTGCGATCAAAGCAGCGCAAACCGGCCATATGGTGATGTCGACCTTACATACCAACAGTGCACCAGAAAC
ACTGACTCGTTTGGCGAATATGGGGGTGCCTAACTTCAGTATTGCAACATCGGTCAGCCTGATTATTGCCCAACGTCTGG
CTCGAAGACTATGCTCAAGTTGCAAGGAGCCTGCCGACCTGCCTCAAGAGGTTCTAAGAGAAGCTGGCTTTACTGATGAA
CTCCTGACAGAGGCAGAAATCTATAAACCGGTGGGCTGTGATAATTGCAAAGAAGGTTTTAAAGGCCGTGTTGGAATATA
TGAAGTAGTTAAAATTACGGACCCTATCTCTCGTATTATCATGGAAGGCGGTAATGCTATTGAAATTGCCGATGCTGCCA
ATAGAGAAGGTTTTAATGACTTACGCACCTCAGCACTAATGAAGGTTGCCCAAGGTATTACCAGTTTAGAAGAAGCCAAC
CGAGTAACTACAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

64.248

100

0.642
  pilB Acinetobacter baylyi ADP1

63.186

100

0.632
  pilB Legionella pneumophila strain ERS1305867

53.792

100

0.54
  pilF Neisseria gonorrhoeae MS11

49.385

100

0.497
  pilB Vibrio cholerae strain A1552

52.471

93.097

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.841

99.292

0.485
  pilB Vibrio campbellii strain DS40M4

49.458

98.053

0.485
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.205

93.451

0.366
  pilF Thermus thermophilus HB27

35.714

100

0.363

Multiple sequence alignment