Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   ACLV7A_RS06620 Genome accession   NZ_OZ217344
Coordinates   1345266..1345940 (-) Length   224 a.a.
NCBI ID   WP_000590627.1    Uniprot ID   A0A7G1ISN9
Organism   Streptococcus mitis isolate S. mitis E22     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1340266..1350940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7A_RS06605 (SMIE22_13020) - 1342557..1343486 (-) 930 WP_411863902.1 peptidase U32 family protein -
  ACLV7A_RS06610 (SMIE22_13030) - 1343626..1343913 (+) 288 WP_411863903.1 DUF3270 domain-containing protein -
  ACLV7A_RS06615 (SMIE22_13040) ciaH 1343942..1345276 (-) 1335 WP_411863904.1 two-component system sensor histidine kinase CiaH Regulator
  ACLV7A_RS06620 (SMIE22_13050) ciaR 1345266..1345940 (-) 675 WP_000590627.1 two-component system response regulator CiaR Regulator
  ACLV7A_RS06625 (SMIE22_13060) - 1346047..1348593 (-) 2547 WP_411863905.1 M1 family metallopeptidase -
  ACLV7A_RS06630 (SMIE22_13070) - 1348716..1349156 (-) 441 WP_411863906.1 ASCH domain-containing protein -
  ACLV7A_RS06635 (SMIE22_13080) - 1349098..1350000 (-) 903 WP_411863907.1 putative PEP-binding protein -
  ACLV7A_RS06640 (SMIE22_13090) - 1350042..1350770 (-) 729 WP_411863908.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 224 a.a.        Molecular weight: 25481.30 Da        Isoelectric Point: 4.2907

>NTDB_id=1170297 ACLV7A_RS06620 WP_000590627.1 1345266..1345940(-) (ciaR) [Streptococcus mitis isolate S. mitis E22]
MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLSYGDILINLSTNTVKVEDTPVELLGKEFDLLVY
FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGYILKDVQ

Nucleotide


Download         Length: 675 bp        

>NTDB_id=1170297 ACLV7A_RS06620 WP_000590627.1 1345266..1345940(-) (ciaR) [Streptococcus mitis isolate S. mitis E22]
ATGATAAAAATCTTATTGGTTGAGGATGACCTAGGCCTGTCAAATTCAGTATTTGACTTTTTAGACGATTTTGCGGATGT
CATGCAGGTATTTGACGGGGAAGAAGGTCTCTACGAAGCTGAAAGTGGCGTCTATGACTTGATTTTGCTCGATTTGATGT
TACCTGAAAAAAATGGCTTCCAAGTCTTGAAAGAATTGCGTGAAAAGGGAATTACGACTCCAGTTCTGATCATGACTGCC
AAGGAAAGTTTGGATGACAAGGGACATGGATTTGAACTGGGAGCGGATGATTATCTGACCAAACCTTTCTACCTAGAAGA
ACTCAAAATGCGGATTCAGGCCCTTCTCAAACGTTCAGGTAAATTTAATGAAAATACGCTATCTTATGGAGATATTCTCA
TCAATTTATCAACAAATACCGTTAAGGTTGAAGATACTCCAGTCGAATTGCTGGGGAAAGAGTTCGATTTACTAGTTTAT
TTCCTTCAAAATCAAAATGTTATTTTGCCTAAGACGCAGATTTTTGATCGTCTATGGGGATTTGATAGTGACACAACGAT
TTCAGTTGTCGAAGTCTATGTTTCAAAAGTCCGTAAGAAATTAAAAGGAACCACTTTTGCAGAGAATTTGCAAACCTTGC
GCAGTGTTGGGTATATTTTAAAAGATGTTCAGTAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G1ISN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

97.768

100

0.978
  ciaR Streptococcus pneumoniae D39

97.768

100

0.978
  ciaR Streptococcus pneumoniae R6

97.768

100

0.978
  ciaR Streptococcus pneumoniae TIGR4

97.768

100

0.978
  ciaR Streptococcus mutans UA159

87.946

100

0.879
  covR Lactococcus lactis subsp. lactis strain DGCC12653

37.611

100

0.379
  vicR Streptococcus mutans UA159

36.481

100

0.379

Multiple sequence alignment