Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACNUEH_RS02305 Genome accession   NZ_OZ197095
Coordinates   474158..475216 (-) Length   352 a.a.
NCBI ID   WP_002014066.1    Uniprot ID   N9LI98
Organism   Acinetobacter baumannii isolate 23S01404-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469158..480216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNUEH_RS02280 aroK 469433..470002 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ACNUEH_RS02285 pilQ 470014..472179 (-) 2166 WP_416632294.1 type IV pilus secretin PilQ Machinery gene
  ACNUEH_RS02290 pilP 472242..472769 (-) 528 WP_024436689.1 pilus assembly protein PilP Machinery gene
  ACNUEH_RS02295 pilO 472780..473520 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACNUEH_RS02300 pilN 473517..474158 (-) 642 WP_416632295.1 PilN domain-containing protein Machinery gene
  ACNUEH_RS02305 pilM 474158..475216 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACNUEH_RS02310 ponA 475378..477933 (+) 2556 WP_416632296.1 penicillin-binding protein PBP1a -
  ACNUEH_RS02315 - 477951..478775 (-) 825 WP_153904635.1 putative RNA methyltransferase -
  ACNUEH_RS02320 - 478778..479110 (-) 333 WP_000598371.1 DUF7709 family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39032.59 Da        Isoelectric Point: 4.4618

>NTDB_id=1169432 ACNUEH_RS02305 WP_002014066.1 474158..475216(-) (pilM) [Acinetobacter baumannii isolate 23S01404-6]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1169432 ACNUEH_RS02305 WP_002014066.1 474158..475216(-) (pilM) [Acinetobacter baumannii isolate 23S01404-6]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAATGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCGGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCCTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACTATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTAGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LI98

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

100

100

1
  comM Acinetobacter nosocomialis M2

99.148

100

0.991
  comM Acinetobacter baylyi ADP1

81.534

100

0.815
  pilM Legionella pneumophila strain ERS1305867

42.09

100

0.423

Multiple sequence alignment