Detailed information NTG0116723

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AW02_RS12770
• Genome accession: NZ_CP007165
• Coordinates: 2689900..2690514 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis NJN-6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2684900..2695514

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AW02_RS12745 (AW02_025380)	rpsT	2685200..2685466 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AW02_RS12750 (AW02_025390)	holA	2685483..2686524 (-)	1042	Protein_2550	DNA polymerase III subunit delta	-
AW02_RS20535	-	2686564..2686718 (-)	155	Protein_2551	hypothetical protein	-
AW02_RS19840	-	2686759..2686893 (+)	135	WP_003152870.1	YqzM family protein	-
AW02_RS12760 (AW02_025400)	comEC	2686912..2689263 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AW02_RS12765 (AW02_025410)	-	2689264..2689833 (-)	570	WP_003152868.1	ComE operon protein 2	-
AW02_RS12770 (AW02_025420)	comEA	2689900..2690514 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
AW02_RS12775 (AW02_025430)	comER	2690573..2691394 (+)	822	WP_014305449.1	late competence protein ComER	-
AW02_RS12780 (AW02_025440)	-	2691463..2692200 (-)	738	WP_024085626.1	class I SAM-dependent DNA methyltransferase	-
AW02_RS12785 (AW02_025450)	rsfS	2692197..2692553 (-)	357	WP_003152864.1	ribosome silencing factor	-
AW02_RS12790 (AW02_025460)	yqeK	2692571..2693131 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AW02_RS12795 (AW02_025470)	-	2693121..2693690 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
AW02_RS12800 (AW02_025480)	yhbY	2693701..2693991 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AW02_RS12805 (AW02_025490)	aroE	2693985..2694827 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=116723 AW02_RS12770 WP_041482377.1 2689900..2690514(-) (comEA) [Bacillus velezensis NJN-6]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=116723 AW02_RS12770 WP_041482377.1 2689900..2690514(-) (comEA) [Bacillus velezensis NJN-6]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363