Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AU14_RS13485 Genome accession   NZ_CP007151
Coordinates   2900316..2902379 (+) Length   687 a.a.
NCBI ID   WP_179944710.1    Uniprot ID   W5YJN7
Organism   Marinobacter similis strain A3d10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2897465..2905564 2900316..2902379 within 0


Gene organization within MGE regions


Location: 2897465..2905564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AU14_RS13465 (AU14_13920) - 2897465..2898526 (+) 1062 Protein_2956 pilus assembly protein PilM -
  AU14_RS13470 (AU14_13925) - 2898526..2899092 (+) 567 WP_041341605.1 PilN domain-containing protein -
  AU14_RS13475 (AU14_13930) - 2899089..2899700 (+) 612 WP_041341606.1 type 4a pilus biogenesis protein PilO -
  AU14_RS13480 (AU14_13935) - 2899703..2900251 (+) 549 WP_041341607.1 pilus assembly protein PilP -
  AU14_RS13485 (AU14_13940) pilQ 2900316..2902379 (+) 2064 WP_179944710.1 type IV pilus secretin PilQ Machinery gene
  AU14_RS13490 (AU14_13945) aroK 2902519..2903085 (+) 567 WP_041341608.1 shikimate kinase AroK -
  AU14_RS13495 (AU14_13950) aroB 2903078..2904171 (+) 1094 Protein_2962 3-dehydroquinate synthase -
  AU14_RS23465 - 2904217..2904774 (+) 558 WP_330217905.1 hypothetical protein -
  AU14_RS23470 - 2905052..2905564 (+) 513 WP_330217906.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 687 a.a.        Molecular weight: 73751.82 Da        Isoelectric Point: 4.3371

>NTDB_id=116543 AU14_RS13485 WP_179944710.1 2900316..2902379(+) (pilQ) [Marinobacter similis strain A3d10]
MFKKLNVYVGVIAFGLLSGLANAVTLEDVSFSSLSGDRLEVKLAFDGEPPEPTGYTIERPARIAVDLRDTTNGLGSRSIP
LGSGNAQSMTVVETKDRTRLIFNLVELVPYDTVKSGNSLVMTLGGDAADVSSTASTGSAPQATSSSSRADALAGVDFRRG
KNGEGRVVVDLGSESAAVDLSELGGKIRLTMSGLSVPENLRRRLDVTDFATPVNRIDTFVQDGNAVVEIRPEGNYDYIAY
QSGSEFTVSVEKLTEEEAESRREEKFPYTGEKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVGGSITLRLQNVPWDQAL
DLILKTKGLDKRQIGNVLLVAPADEIAAREKLELETTKQIAELAPVRLDIIQVNYAKAADVVALIEADKELISDRGFVSS
DVRTNTISVRETTEKLEEIRRLVTTWDVPVRQVSIEARIVRAQTNVAENLGIRWGGAAYNVSGDNVFSVGGSQGAVEEAR
QAAGGTNNTITFPGALAVDLGVTGEGASSFALGWGSDDFLVDLELSALESDGQAEVVSQPRVVTADRQTASIKSGEEIPY
QEASSSGATSVSFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNEVTTQVLVGNGETVVLGGIFQSEV
ATQTTKTPFLGDIPYLGRLFKRTEHVDERSELLIFITPKILKNDLIR

Nucleotide


Download         Length: 2064 bp        

>NTDB_id=116543 AU14_RS13485 WP_179944710.1 2900316..2902379(+) (pilQ) [Marinobacter similis strain A3d10]
ATGTTTAAAAAACTCAATGTATACGTCGGCGTGATTGCTTTTGGGTTGTTATCCGGCCTGGCCAATGCGGTCACGCTGGA
AGACGTGTCATTCTCATCGCTATCAGGAGACCGACTGGAGGTTAAGCTTGCCTTCGATGGCGAGCCTCCCGAACCGACGG
GATACACAATTGAGCGGCCGGCTCGAATTGCTGTGGACTTGCGCGATACCACCAATGGGCTCGGTAGTCGCAGTATCCCG
CTGGGCTCAGGCAATGCTCAGAGCATGACAGTCGTTGAAACCAAGGACCGGACGCGCCTGATCTTTAATCTGGTCGAACT
GGTTCCCTATGACACGGTCAAAAGTGGGAACTCCCTGGTGATGACTCTTGGGGGCGATGCAGCTGACGTTTCGTCGACGG
CGTCAACTGGCTCTGCGCCGCAAGCAACTTCCTCATCATCTCGTGCAGATGCTCTTGCCGGAGTTGATTTCCGTCGCGGC
AAAAACGGAGAAGGTCGGGTAGTGGTCGATCTGGGCAGCGAGAGTGCTGCGGTGGATTTGTCCGAATTGGGTGGAAAGAT
TCGGCTGACCATGTCAGGCCTTTCCGTGCCAGAAAATCTCCGTCGGCGTCTGGATGTGACGGATTTTGCGACGCCGGTTA
ATCGCATCGATACGTTTGTCCAAGATGGCAACGCCGTCGTCGAAATCCGGCCGGAAGGTAATTACGACTACATCGCGTAT
CAGTCTGGTAGCGAATTCACGGTCAGTGTTGAAAAGCTGACAGAGGAGGAAGCGGAATCCCGTCGTGAGGAGAAATTCCC
GTACACGGGTGAGAAGCTGTCCCTTAACTTCCAGGATATTGAAGTTCGCTCTGTTCTTCAGTTAATTGCAGATTTCACCG
GACTGAACCTGGTCGCCAGCGATACTGTGGGCGGAAGCATTACCCTGCGGCTCCAAAATGTCCCTTGGGACCAGGCATTG
GATCTGATCCTGAAAACTAAAGGTCTCGACAAGCGCCAAATTGGTAACGTACTCTTGGTAGCGCCTGCGGATGAGATCGC
CGCGCGTGAGAAGCTGGAGCTGGAAACTACCAAACAGATTGCAGAACTTGCTCCGGTTCGTCTGGATATCATCCAGGTGA
ATTATGCCAAGGCTGCAGATGTAGTCGCTTTGATTGAGGCGGATAAAGAGCTGATTTCCGACCGTGGTTTCGTCTCTTCG
GATGTTCGGACGAATACTATCAGCGTGCGGGAAACCACAGAGAAGCTGGAAGAAATCCGCCGACTCGTTACTACCTGGGA
CGTTCCAGTTCGCCAGGTATCTATCGAAGCCAGGATCGTTCGTGCTCAGACCAACGTTGCCGAAAACCTTGGTATTCGCT
GGGGCGGTGCGGCCTACAATGTGAGTGGCGATAACGTTTTCTCAGTTGGTGGTTCTCAGGGCGCGGTCGAAGAGGCCCGT
CAGGCCGCCGGTGGCACCAACAACACAATCACTTTCCCGGGCGCTCTGGCAGTGGATCTGGGGGTAACGGGTGAGGGTGC
CTCCTCATTCGCGCTGGGTTGGGGTAGTGACGACTTCCTGGTCGACCTGGAACTGTCCGCTCTCGAAAGCGACGGACAGG
CGGAAGTTGTATCGCAGCCGCGAGTTGTGACTGCGGATCGCCAGACAGCGTCGATCAAATCTGGTGAAGAGATTCCTTAC
CAGGAAGCGTCCTCAAGCGGTGCCACCTCTGTGTCCTTCAAGGAAGCGGTGTTGTCGCTGGAGGTAACTCCGCAGATCAC
GCCTGATGACAAGATCATCATGGATCTGGTGGTGAACCAGGACTCCCGCGGTGAGGTAACTGCAGGCATTCCGTCAATCA
ACACCAATGAAGTTACGACTCAGGTGCTGGTTGGCAACGGCGAAACCGTGGTGTTGGGCGGTATCTTCCAGTCGGAGGTG
GCCACCCAAACCACCAAGACACCGTTCCTGGGTGATATCCCGTACCTCGGTCGTCTGTTCAAGCGGACTGAGCATGTGGA
TGAGCGCAGTGAGCTGCTGATCTTCATCACGCCCAAGATCCTGAAGAACGATCTGATCCGCTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(296-342)

Bacterial type II and III secretion system protein (Secretin)

(527-682)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W5YJN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

51.079

100

0.517
  comQ Acinetobacter baylyi ADP1

40.793

100

0.419
  pilQ Acinetobacter baumannii D1279779

39.889

100

0.419
  pilQ Acinetobacter baumannii strain A118

39.889

100

0.419
  pilQ Legionella pneumophila strain Lp02

42.121

98.836

0.416
  pilQ Legionella pneumophila strain ERS1305867

42.121

98.836

0.416

Multiple sequence alignment