Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   YC6258_RS10630 Genome accession   NZ_CP007142
Coordinates   2455267..2456964 (-) Length   565 a.a.
NCBI ID   WP_044616971.1    Uniprot ID   A0A0C5VVF6
Organism   Gynuella sunshinyii YC6258     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2450267..2461964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YC6258_RS10605 (YC6258_02401) argJ 2451101..2452324 (+) 1224 WP_044616966.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  YC6258_RS10610 yacG 2452352..2452570 (-) 219 WP_211264666.1 DNA gyrase inhibitor YacG -
  YC6258_RS10615 (YC6258_02403) coaE 2452567..2453166 (-) 600 WP_044616968.1 dephospho-CoA kinase -
  YC6258_RS10620 (YC6258_02404) pilD 2453163..2454029 (-) 867 WP_044616969.1 A24 family peptidase Machinery gene
  YC6258_RS10625 (YC6258_02405) pilC 2454029..2455252 (-) 1224 WP_044616970.1 type II secretion system F family protein Machinery gene
  YC6258_RS10630 (YC6258_02406) pilB 2455267..2456964 (-) 1698 WP_044616971.1 type IV-A pilus assembly ATPase PilB Machinery gene
  YC6258_RS10635 (YC6258_02407) - 2457219..2457719 (+) 501 WP_044616972.1 pilin -
  YC6258_RS10640 (YC6258_02408) - 2457796..2459412 (+) 1617 WP_144407611.1 Wzy polymerase domain-containing protein -
  YC6258_RS10645 (YC6258_02409) nadC 2459655..2460518 (-) 864 WP_044619948.1 carboxylating nicotinate-nucleotide diphosphorylase -
  YC6258_RS10650 (YC6258_02410) ampE 2460708..2461556 (+) 849 WP_044616974.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62186.44 Da        Isoelectric Point: 5.3558

>NTDB_id=116474 YC6258_RS10630 WP_044616971.1 2455267..2456964(-) (pilB) [Gynuella sunshinyii YC6258]
MAALLNGLSKRLVADQIISEESAIEATSLAKKNKVQLVTQLIDSELATPLQIAQTCATEFGTPLFDLEAFDKELLNKELV
DSKLIIAHRAFPLIKRGNRLFVAVSDPTNLIGLDEIKFHTGLSTESILVEDDKLSRLIESFLDDGNDAFEGLEDEALDNL
EVAETKKDEGDEGNSGADDAPVVRFINQMLLSAIKKGASDLHFEPYEKTYRVRFRIDGMLQDMSRPPVALGPKIAARLKV
MSQLDISERRVPQDGRIKLKISKTKAIDFRVNTLPTLFGEKIVLRILDSSSAKMGIDALGYEPDQKEMYLEALKRPQGMI
LVTGPTGSGKTVSLYTGLNILNTEGVNISTAEDPVEINLEGINQVNVNNRVGLDFASALRSFLRQDPDVIMVGEIRDLET
ASIAIKAAQTGHMVLSTLHTNSAAETLTRMLNMGVPSFNLATTVNLIIAQRLARRLCGHCKQALDVPVETLKKEGFSDEM
LKSAKIYHPVGCNQCNKGYKGRVGIYEVVKITPAISRIIMEEGNSIQLNEQFKKEGYNNLRKSGLVKVCQGVTSLEEVNR
VTSEN

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=116474 YC6258_RS10630 WP_044616971.1 2455267..2456964(-) (pilB) [Gynuella sunshinyii YC6258]
ATGGCAGCACTACTAAACGGCTTATCCAAACGATTGGTTGCAGATCAGATTATTAGTGAAGAATCAGCTATTGAAGCAAC
CTCACTAGCCAAAAAAAATAAAGTACAACTCGTCACTCAGTTGATAGATTCAGAACTGGCAACTCCTCTACAGATAGCCC
AAACTTGCGCAACTGAATTTGGCACTCCTTTATTCGACCTGGAAGCTTTTGACAAAGAGCTACTTAATAAAGAACTTGTT
GATAGTAAATTAATAATCGCTCATCGTGCTTTCCCTTTGATAAAAAGAGGGAATCGCCTCTTTGTTGCCGTATCAGATCC
AACCAACTTAATCGGATTAGATGAAATAAAATTTCATACAGGATTAAGTACAGAGTCAATCCTGGTTGAGGATGACAAGC
TTTCTCGTTTGATTGAGAGCTTTCTCGATGACGGTAACGACGCCTTCGAAGGCCTAGAAGATGAAGCCTTGGATAATTTA
GAAGTCGCAGAAACTAAAAAGGATGAAGGAGATGAGGGAAATAGTGGTGCAGATGATGCTCCTGTTGTCCGCTTTATTAA
TCAAATGCTGCTCTCAGCGATTAAGAAAGGCGCTTCAGACCTACACTTTGAACCTTACGAAAAAACATATCGCGTCCGCT
TCCGTATTGATGGAATGTTACAGGATATGTCTCGCCCACCCGTTGCTCTTGGCCCTAAAATCGCCGCACGACTAAAAGTA
ATGTCTCAACTGGACATTTCTGAGCGCAGGGTTCCCCAGGATGGACGTATAAAACTCAAAATATCAAAGACCAAAGCTAT
TGACTTTCGTGTAAATACACTGCCCACACTGTTTGGCGAAAAGATCGTATTGCGAATTCTCGATTCCAGTAGCGCTAAAA
TGGGAATTGACGCTTTAGGTTATGAGCCGGATCAAAAGGAAATGTACCTGGAGGCACTCAAACGTCCTCAGGGAATGATA
CTGGTTACTGGCCCGACAGGATCCGGAAAAACTGTATCTCTGTATACTGGTTTAAATATATTGAACACTGAAGGTGTTAA
TATTTCTACAGCCGAGGACCCTGTTGAAATTAACCTGGAAGGGATTAACCAAGTTAACGTCAATAATAGAGTAGGGCTCG
ATTTTGCGTCAGCACTAAGATCATTTTTGAGGCAGGACCCCGATGTCATTATGGTTGGAGAAATCAGGGACCTGGAAACA
GCGTCTATCGCTATCAAAGCAGCTCAAACGGGCCATATGGTACTCTCTACCCTTCATACCAACAGTGCGGCAGAAACATT
GACTCGTATGCTGAATATGGGTGTCCCGTCATTTAACCTGGCAACGACCGTCAATTTGATTATCGCCCAACGACTCGCAC
GCAGATTATGTGGTCACTGTAAACAAGCCTTAGATGTTCCCGTTGAAACATTGAAAAAGGAAGGCTTCAGTGACGAAATG
CTCAAAAGCGCCAAGATTTATCACCCCGTAGGTTGCAATCAATGCAACAAAGGTTATAAAGGGCGAGTAGGTATTTATGA
AGTCGTTAAGATTACCCCGGCAATTTCCAGAATTATTATGGAAGAAGGTAATTCAATTCAATTGAATGAACAATTCAAAA
AAGAAGGCTATAACAACCTGCGTAAATCCGGATTGGTAAAAGTGTGTCAAGGTGTTACCAGCCTTGAAGAAGTCAACCGC
GTTACATCGGAAAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C5VVF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

61.17

99.823

0.611
  pilB Acinetobacter baumannii D1279779

60.424

100

0.605
  pilB Legionella pneumophila strain ERS1305867

53.996

99.646

0.538
  pilB Vibrio parahaemolyticus RIMD 2210633

51.548

97.168

0.501
  pilB Vibrio cholerae strain A1552

55.098

90.265

0.497
  pilB Vibrio campbellii strain DS40M4

49.82

98.407

0.49
  pilF Neisseria gonorrhoeae MS11

49.288

99.469

0.49
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.306

96.106

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

41

88.496

0.363

Multiple sequence alignment