Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLMBQ_RS09530 Genome accession   NZ_OY829390
Coordinates   2073396..2075108 (+) Length   570 a.a.
NCBI ID   WP_138439338.1    Uniprot ID   -
Organism   MAG: Marinobacter alexandrii isolate 78648b50-2abc-418f-b92f-8dc6f39ea282     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2068396..2080108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBQ_RS09510 (U5725_RS09515) - 2068932..2070293 (+) 1362 WP_322003694.1 MBL fold metallo-hydrolase -
  ACLMBQ_RS09515 (U5725_RS09520) - 2070341..2070784 (-) 444 WP_322003696.1 pilin -
  ACLMBQ_RS09520 (U5725_RS09525) - 2070858..2072396 (-) 1539 WP_322003697.1 O-antigen ligase family protein -
  ACLMBQ_RS09525 (U5725_RS09530) - 2072546..2073001 (-) 456 WP_322003698.1 pilin -
  ACLMBQ_RS09530 (U5725_RS09535) pilB 2073396..2075108 (+) 1713 WP_138439338.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLMBQ_RS09535 (U5725_RS09540) pilC 2075111..2076334 (+) 1224 WP_322003700.1 type II secretion system F family protein Machinery gene
  ACLMBQ_RS09540 (U5725_RS09545) pilD 2076402..2077277 (+) 876 WP_322003701.1 A24 family peptidase Machinery gene
  ACLMBQ_RS09545 (U5725_RS09550) coaE 2077281..2077880 (+) 600 WP_322003702.1 dephospho-CoA kinase -
  ACLMBQ_RS09550 (U5725_RS09555) - 2077873..2078967 (+) 1095 WP_322003703.1 hypothetical protein -
  ACLMBQ_RS09555 (U5725_RS09560) tsaA 2078970..2079713 (+) 744 WP_138439333.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62669.51 Da        Isoelectric Point: 4.9015

>NTDB_id=1162078 ACLMBQ_RS09530 WP_138439338.1 2073396..2075108(+) (pilB) [MAG: Marinobacter alexandrii isolate 78648b50-2abc-418f-b92f-8dc6f39ea282]
MATNNSNVTLTGLARRFVDDGLLDEATAKDAFLQASQNRTPLITYLVQNNLANSSGLAFSAAMEFGVSVLDLSSFLPEMM
PEKTVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAVLVDDAKLRAAIDKYLESQDTTMGDLDDA
DLEGVETEGGEQEDDGAVSTTEVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKTYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKEMYMTALE
QPQGMILVTGPTGSGKTVSLYTGINILNTHERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCSCKQATDIPKDVLLKEG
FTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAQQAQAEGFRNLRQSALLKVIEGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1162078 ACLMBQ_RS09530 WP_138439338.1 2073396..2075108(+) (pilB) [MAG: Marinobacter alexandrii isolate 78648b50-2abc-418f-b92f-8dc6f39ea282]
ATGGCTACCAACAACAGCAATGTAACGCTCACCGGTCTCGCAAGACGCTTCGTGGACGACGGCCTGCTGGATGAAGCAAC
GGCGAAAGACGCGTTTCTTCAAGCTTCCCAGAACCGCACCCCCCTGATCACCTATTTGGTACAGAATAATCTTGCAAACA
GTAGTGGGCTGGCGTTTTCTGCGGCAATGGAGTTCGGGGTTTCGGTTCTGGATCTCAGTTCTTTTTTGCCGGAAATGATG
CCCGAGAAGACTGTCGATGAGAAACTGATCCGAAAGCACAATGCCTTACCGCTCTACAAGAGAGGCAACCGCCTATTTAT
AGCCGTGTCTGACCCGACTAACATTCAGGCGCTGGACGAAATAAAGTTTAATACCGGCCTGAGTACCGATGCAGTCCTGG
TCGACGACGCCAAACTTCGTGCGGCGATCGACAAGTACCTCGAGTCGCAAGACACCACCATGGGTGATCTCGACGATGCC
GATTTGGAAGGCGTCGAGACTGAGGGCGGCGAACAGGAAGACGACGGCGCAGTCTCCACGACTGAGGTCGATGATGCCCC
CATTGTTAAGTACGTCAACAAAATGCTGCTTGACGCCATTCGTGGCGGCGCCTCCGATGTTCACTTCGAACCGTACGAAA
AGACTTACCGGGTGCGTTACAGAACCGACGGCATCCTCAAAGAAGTGTCCCGACCGTCCATCAAACTGGCCCCCAAAATT
TCTGCGCGCGTAAAGATCATGGCGCAGCTGGACATTTCAGAACGCCGCGTACCTCAGGATGGCCGCATCAAGATGAAGCT
ATCCAAAACCAAAGCCATCGATTTCCGGGTCAACACTCTCCCCACCCTCTGGGGTGAGAAGATCGTGCTGCGGATTCTTG
ACCCAAGCCAAGCCAAACTCGGCATTGATGTACTGGGTTACGAAGAAGACCAGAAAGAGATGTATATGACTGCTCTTGAA
CAACCGCAGGGCATGATTCTTGTGACTGGACCGACCGGTTCGGGCAAGACCGTTTCTCTCTATACCGGTATCAACATTCT
GAACACTCATGAGCGCAACATTTCTACGGCCGAGGACCCTGCGGAAATCAACCTTGAAGGCATAAACCAGGTTAACGTGA
ATACGCGGGTCGGACTTGGCTTCGCTGAAGCCCTGCGGGCATTCCTGCGACAAGATCCCGACGTCATCATGGTAGGCGAG
ATTCGAGACCTTGAAACCGCCAACATTGCCATAAAGGCTGCCCAAACCGGCCACCTCGTCCTTTCAACCCTTCACACCAA
CAGTGCCGCCGAAACACTGACACGGATGATGAACATGGGTGTGCCATCATTCAATATTGCAACATCGGTGAGCCTGATCA
TCGCCCAGCGCCTTGGCCGGCGCCTGTGTAGCTGCAAGCAGGCAACGGACATTCCCAAGGACGTACTTTTGAAGGAAGGG
TTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCAAAAGGCTGTGATAAATGCAATGGTGGATATAAAGG
CCGCGTTGGTATCTACGAAGTGGTCAAGATCACCGACGAGCTGGCGAACATGATTATGGAAGAGGCCAGCTCCATTAAAA
TCGCACAGCAGGCTCAGGCCGAAGGCTTCAGAAACTTGCGTCAGTCGGCGCTTTTGAAGGTTATTGAGGGTGTCACCAGC
CTCGAAGAAGCCAACCGGGTCACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.643

100

0.568
  pilB Acinetobacter baylyi ADP1

55.769

100

0.56
  pilB Legionella pneumophila strain ERS1305867

53.391

100

0.539
  pilB Vibrio cholerae strain A1552

51.246

98.596

0.505
  pilB Vibrio campbellii strain DS40M4

49.468

98.947

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

52.174

92.807

0.484
  pilF Neisseria gonorrhoeae MS11

47.34

98.947

0.468
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.113

93.158

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

40.154

90.877

0.365

Multiple sequence alignment