Detailed information NTG0116197

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BH81_RS13955
• Genome accession: NZ_CP007039
• Coordinates: 3342328..3343491 (-)
• Length: 387 a.a.
• NCBI ID: WP_025260389.1
• Uniprot ID: -
• Organism: Pseudomonas cichorii JBC1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3337328..3348491

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BH81_RS13940 (PCH70_27970)	-	3338452..3339864 (+)	1413	WP_025260386.1	sigma-54-dependent Fis family transcriptional regulator	-
BH81_RS13945 (PCH70_27980)	-	3340107..3341498 (+)	1392	WP_025260387.1	GntP family permease	-
BH81_RS13950 (PCH70_27990)	-	3341509..3342270 (+)	762	WP_038399888.1	3-hydroxybutyrate dehydrogenase	-
BH81_RS13955 (PCH70_28000)	pilU	3342328..3343491 (-)	1164	WP_025260389.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BH81_RS13960 (PCH70_28010)	-	3343624..3343899 (+)	276	WP_025260390.1	peptidylprolyl isomerase	-
BH81_RS13965 (PCH70_28020)	pgm	3343954..3345600 (-)	1647	WP_025260391.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
BH81_RS13970 (PCH70_28030)	-	3345703..3348030 (-)	2328	WP_025260392.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42683.86 Da
• Isoelectric Point: 6.2919

>NTDB_id=116197 BH81_RS13955 WP_025260389.1 3342328..3343491(-) (pilU) [Pseudomonas cichorii JBC1]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGTETLKPGEVAIVAQGLMDEEQKLEFQRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLHLPPILLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERREQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNQLTELKGIMEKSGSLGMQTFDTAL
FNLAAEGVISEEEALKNADSQNNVRLRLKLHGEGVSHLTTAPSAPVGTNTAQMPDWGLVDENEGPQS

Nucleotide

• Length: 1164 bp

>NTDB_id=116197 BH81_RS13955 WP_025260389.1 3342328..3343491(-) (pilU) [Pseudomonas cichorii JBC1]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGCCAGGACGGATCGGACCTTTATCTTTCCACCGGAGCGCCGCCTTG
CGCCAAGTTCAATGGCGTGCTCAAGCCGCTGGGCACTGAAACCCTCAAGCCCGGTGAGGTGGCGATCGTCGCCCAAGGCC
TGATGGATGAGGAACAGAAGCTCGAATTCCAGCGTGAGTTGGAGATGAACTTGGCGGTGTCGCTGGCCGGTATCGGGCGT
TTCCGGATCAATATCTTCATGCAACGCAACGAAGTCTCCATCGTTGCGCGCAATATCAAACTGGATATCCCGCGTTTCGA
GGACCTGCACCTGCCGCCGATCCTGCTCGATGTGATCATGGAAAAACACGGGCTGGTGCTGTTCGTAGGCGCGACTGGCT
CGGGTAAATCTACTTCCCTGGCGGCCCTGATCGATTACCGTAACCGCAATGCCAGTGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTTCATCCATCGCCACAAGAAGTCCATCGTCAACCAGCGTGAAGTGGGGGTCGATACCCGCAGTTTCCGTGC
CGCCCTGAAAAACACCTTGCGTCAGGCACCGGATGTCATCCTGATCGGCGAGATTCGTGACCGGGAAACCATGGAGCATG
CGCTGGCCTTTGCCGATACCGGTCACCTGGCCATCTCGACCCTGCATGCCAACAACGCCAACCAGGCCCTGGACCGAATC
ATCAATTTCTTCCCGGAAGAGCGCCGCGAACAACTGCTGCACGACCTGGGCAATAACCTCAAGGCCTTCGTTTCCCAGCG
ACTGGTCAAGACCCCGGATGGAAAACGACGGGCTGCTGTTGAGGTGATGATGGGCACGCCAACCATTCGTGATCTGATCC
AGCGCAACCAACTGACTGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTGGGCATGCAGACCTTCGATACCGCGCTG
TTCAACCTGGCGGCAGAAGGGGTCATCAGCGAAGAGGAAGCCCTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAGTTGCATGGCGAGGGCGTCTCGCACCTGACAACGGCACCCTCGGCGCCTGTGGGGACGAATACAGCGCAGATGC
CCGATTGGGGCCTCGTGGATGAAAATGAAGGCCCCCAATCCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.182	93.54	0.535
pilU	Acinetobacter baylyi ADP1	51.966	91.99	0.478
pilU	Vibrio cholerae strain A1552	50.857	90.439	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.51	94.832	0.375
pilT	Pseudomonas aeruginosa PAK	41.108	88.63	0.364
pilT	Pseudomonas stutzeri DSM 10701	40.816	88.63	0.362