Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ONG87_RS17595 Genome accession   NZ_OX352319
Coordinates   3870968..3872668 (+) Length   566 a.a.
NCBI ID   WP_264381752.1    Uniprot ID   -
Organism   Pseudomonas sp. MM227     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3865968..3877668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONG87_RS17545 (AHFPHNDE_03491) - 3866042..3866641 (-) 600 WP_122475276.1 LemA family protein -
  ONG87_RS17550 (AHFPHNDE_03492) - 3866902..3867120 (-) 219 WP_192058563.1 hypothetical protein -
  ONG87_RS17555 (AHFPHNDE_03493) - 3867245..3867424 (+) 180 WP_122475279.1 hypothetical protein -
  ONG87_RS17560 (AHFPHNDE_03494) - 3867480..3867725 (-) 246 WP_264381749.1 hypothetical protein -
  ONG87_RS17565 (AHFPHNDE_03495) - 3867892..3868659 (+) 768 WP_264381750.1 hypothetical protein -
  ONG87_RS17570 (AHFPHNDE_03496) - 3868656..3869066 (-) 411 WP_264381751.1 VOC family protein -
  ONG87_RS17575 (AHFPHNDE_03497) - 3869538..3869843 (+) 306 WP_192137714.1 DUF1484 family protein -
  ONG87_RS17590 pilE 3870256..3870681 (+) 426 WP_301335539.1 pilin Machinery gene
  ONG87_RS17595 (AHFPHNDE_03500) pilB 3870968..3872668 (+) 1701 WP_264381752.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ONG87_RS17600 pilC 3872672..3873886 (+) 1215 WP_264381753.1 type II secretion system F family protein Machinery gene
  ONG87_RS17605 (AHFPHNDE_03502) pilD 3873888..3874757 (+) 870 WP_264381754.1 A24 family peptidase Machinery gene
  ONG87_RS17610 (AHFPHNDE_03503) coaE 3874754..3875377 (+) 624 WP_264381755.1 dephospho-CoA kinase -
  ONG87_RS17615 (AHFPHNDE_03504) yacG 3875374..3875577 (+) 204 WP_192102642.1 DNA gyrase inhibitor YacG -
  ONG87_RS17620 (AHFPHNDE_03505) - 3875767..3876192 (-) 426 WP_264381756.1 DUF3830 family protein -
  ONG87_RS17625 (AHFPHNDE_03506) - 3876293..3876922 (+) 630 WP_122475299.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62546.50 Da        Isoelectric Point: 6.0081

>NTDB_id=1155290 ONG87_RS17595 WP_264381752.1 3870968..3872668(+) (pilB) [Pseudomonas sp. MM227]
MNEVVLSGLAKHLVQAELLTEIVAQQAQQRALKEKIGFVHYLVQNKLAKGREIAEIASDQFGVSLIDVTAFDPEHQPKGL
VSEKLIRQHHVLPLWRRGNKLFIGISDPTNHQSITDIQFSTGLNTEAILVSDEQLTSAIEKYFDTNATGLESLADIDLDG
LDIESVDDHKTEPVAGQDSDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLKEVARPPINLAGRISARL
KVMANLDISERRRPQDGRIKMRISKNKSIDFRVNTLPTLWGEKIVMRILDPSSAQLGIDVLGYEADQKELYLQALSQPQG
MILVTGPTGSGKTVSLYTGLNILNTIDINISTAEDPVELNMEGINQVNVNNRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLQNMGVASFNIATSVSLIIAQRLARKLCPHCKREANIPQEALLAEGFPE
NAVSGLKIYEPVGCDQCNGGYRGRQGIYEVVKNTKALQQLIMEGANAIMIDEQMRKDGFNSLRTSGLRKVIQGVTSLAEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1155290 ONG87_RS17595 WP_264381752.1 3870968..3872668(+) (pilB) [Pseudomonas sp. MM227]
ATGAACGAAGTGGTCCTTTCCGGATTAGCCAAACATTTAGTTCAAGCAGAGCTCTTGACCGAGATAGTTGCTCAGCAGGC
ACAGCAGCGTGCCTTAAAAGAGAAAATAGGTTTCGTACACTATTTAGTCCAGAATAAACTTGCCAAGGGACGTGAGATTG
CAGAAATAGCGTCCGACCAGTTCGGCGTATCATTAATTGATGTCACTGCTTTCGACCCTGAACATCAGCCAAAAGGGTTA
GTCAGTGAAAAATTGATTCGACAGCATCATGTACTACCTTTATGGCGGAGGGGAAACAAGTTATTTATAGGAATATCTGA
CCCTACAAATCATCAATCTATCACCGACATACAGTTCAGCACAGGACTGAACACAGAAGCCATTCTTGTTTCTGATGAGC
AACTGACCTCTGCTATAGAGAAATATTTCGACACCAATGCAACTGGTCTTGAATCATTAGCAGACATAGATCTAGATGGA
CTGGATATTGAGAGCGTAGATGATCACAAGACAGAGCCAGTTGCGGGTCAAGATTCGGACGACGCTCCAGTGGTCAGGTT
TGTAAACAAAATGTTGTTGGACGCTATCCGCGGAGGCTCATCCGATCTCCACTTTGAGCCTTACGAAAAAATATATCGTG
TACGCTTTCGAACGGATGGCATGTTAAAAGAAGTGGCTCGCCCCCCAATAAACTTGGCGGGTAGAATTTCTGCCCGTTTA
AAAGTAATGGCCAACCTCGATATCTCAGAAAGACGTAGACCGCAAGATGGCCGTATCAAAATGCGAATCTCAAAAAATAA
ATCGATCGATTTCCGAGTTAACACTTTGCCTACCCTGTGGGGCGAAAAGATAGTAATGCGGATATTGGACCCGTCCAGCG
CACAACTCGGGATTGATGTTTTAGGGTATGAGGCCGACCAAAAAGAGCTCTACCTTCAGGCTCTTAGCCAACCACAAGGC
ATGATCCTAGTAACTGGGCCGACTGGTTCTGGTAAAACCGTATCGCTGTACACTGGATTGAATATACTCAATACCATAGA
TATAAACATATCGACTGCCGAGGATCCAGTCGAACTCAATATGGAAGGAATAAACCAGGTAAACGTAAATAATCGTCAAG
GTTTAGATTTTGCCCAAGCACTACGATCATTCCTGCGCCAAGATCCGGACGTTATCATGGTCGGCGAAATTCGAGATCTT
GAGACTGCTGAAATCGCTATAAAAGCAGCGCAGACAGGTCATTTGGTACTTTCTACCCTACATACGAATAGTGCAGCGGA
GACACTAACTCGCCTGCAGAACATGGGCGTAGCCAGCTTTAACATTGCCACATCTGTCAGCCTGATAATCGCCCAGCGCC
TAGCTCGAAAGCTATGCCCACACTGCAAAAGAGAAGCTAACATTCCTCAAGAAGCTTTGCTAGCCGAAGGCTTTCCCGAG
AATGCTGTGAGCGGCCTAAAAATATATGAGCCGGTAGGATGCGACCAGTGCAATGGAGGCTATCGTGGACGTCAAGGCAT
CTATGAAGTTGTTAAAAATACCAAGGCACTACAACAGCTAATTATGGAAGGCGCTAACGCGATAATGATTGATGAGCAGA
TGCGCAAGGATGGCTTTAATAGCTTACGAACTTCCGGCCTGAGAAAAGTCATTCAGGGCGTAACCAGCCTCGCAGAAATC
AACCGGGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.42

100

0.574
  pilB Acinetobacter baumannii D1279779

55.851

99.647

0.557
  pilB Legionella pneumophila strain ERS1305867

54.93

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

49.474

100

0.498
  pilB Vibrio cholerae strain A1552

50.267

99.117

0.498
  pilB Vibrio campbellii strain DS40M4

49.204

99.823

0.491
  pilF Neisseria gonorrhoeae MS11

48.233

100

0.482

Multiple sequence alignment