Detailed information NTG1155281

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ONG87_RS08995
• Genome accession: NZ_OX352319
• Coordinates: 1969991..1971154 (+)
• Length: 387 a.a.
• NCBI ID: WP_122478440.1
• Uniprot ID: -
• Organism: Pseudomonas sp. MM227
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1964991..1976154

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ONG87_RS08975 (AHFPHNDE_01792)	-	1965868..1966746 (-)	879	WP_122478446.1	Ku protein	-
ONG87_RS08980 (AHFPHNDE_01793)	-	1967000..1967257 (+)	258	WP_122714198.1	DUF1652 domain-containing protein	-
ONG87_RS08985 (AHFPHNDE_01794)	-	1967444..1967719 (-)	276	WP_043189034.1	peptidylprolyl isomerase	-
ONG87_RS08990 (AHFPHNDE_01795)	-	1967762..1969903 (-)	2142	WP_264382691.1	ATP-binding protein	-
ONG87_RS08995 (AHFPHNDE_01796)	pilU	1969991..1971154 (+)	1164	WP_122478440.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ONG87_RS09000 (AHFPHNDE_01797)	-	1971403..1972203 (-)	801	WP_264382692.1	endonuclease/exonuclease/phosphatase family protein	-
ONG87_RS09005 (AHFPHNDE_01798)	hbdH	1972268..1973038 (-)	771	WP_264382846.1	3-hydroxybutyrate dehydrogenase	-
ONG87_RS09010 (AHFPHNDE_01799)	-	1973078..1974655 (-)	1578	WP_264382693.1	ABC transporter ATP-binding protein	-
ONG87_RS09015 (AHFPHNDE_01801)	-	1974809..1975831 (-)	1023	WP_122479667.1	ABC transporter permease	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42873.84 Da
• Isoelectric Point: 6.4385

>NTDB_id=1155281 ONG87_RS08995 WP_122478440.1 1969991..1971154(+) (pilU) [Pseudomonas sp. MM227]
MEIQALLKLLASQDGSDLYLSTGAPPCAKFNGVLKPLSAEALKPGDIAAIAASIMDAEQRIDFERELEMNLALSIHGVGR
FRVNIFKQRNEVSIVARNIKLDIPSFEDLKLPRKLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFQAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLQAFVSQRLVRTTDGKRRAAVEVMLGTPTIRDFIHRNEFSELKGIMEKSVNLGMQTFDHAL
YELAIEGAISEEEALKNADSVNNLRLRLKLHGEEGVQHTSAPAPPTPASDRKSADWGLVSDDPESQQ

Nucleotide

• Length: 1164 bp

>NTDB_id=1155281 ONG87_RS08995 WP_122478440.1 1969991..1971154(+) (pilU) [Pseudomonas sp. MM227]
ATGGAAATCCAGGCATTGCTCAAGCTCCTGGCCAGTCAGGACGGCTCCGACCTCTACCTGTCCACTGGCGCGCCACCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTCAGCGCCGAAGCCTTGAAACCAGGCGACATTGCCGCGATCGCGGCCAGTA
TCATGGACGCCGAGCAGCGTATCGACTTCGAGCGTGAGCTGGAAATGAATCTGGCGTTGTCGATCCACGGGGTAGGACGC
TTTCGCGTCAATATCTTCAAGCAGCGCAACGAAGTGTCCATTGTCGCACGCAACATCAAGCTCGACATTCCCAGCTTCGA
AGACCTCAAGCTGCCGCGCAAGCTGCTCGACGTGATCATGGAAAAGCGCGGCCTGGTGCTGTTCGTCGGCGCGACCGGTT
CCGGCAAGTCGACGTCGCTGGCCGCATTGATCGACTACCGCAACCGCAACAGCAGCGGGCACATCATCACCATCGAGGAC
CCGGTGGAGTTCATCCACCGGCACAAGAAGTCGATCATCAACCAGCGTGAAGTGGGGGTCGACACGCGCAGCTTCCAGGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAACACG
CACTGGCATTCGCCGACACCGGCCACCTGGCGATTTCCACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCCGAAGACCGCCGCCCGCAGTTGCTCAACGATCTGGGCAACAACCTGCAGGCGTTCGTCTCGCAGCG
GCTGGTACGCACCACCGACGGCAAGCGCCGCGCCGCGGTCGAGGTGATGCTGGGCACGCCGACGATTCGCGACTTCATTC
ACCGCAACGAGTTTTCCGAGCTCAAGGGCATCATGGAGAAATCGGTGAACCTGGGCATGCAGACGTTCGACCATGCTTTG
TACGAGCTGGCCATCGAGGGGGCGATCAGCGAGGAAGAAGCGTTAAAGAATGCCGATTCGGTGAACAACCTGCGCCTGCG
CTTGAAGCTGCATGGCGAGGAGGGCGTGCAGCACACCTCGGCGCCCGCCCCGCCCACGCCTGCATCCGACCGCAAAAGCG
CAGATTGGGGCCTGGTGAGCGACGACCCCGAAAGCCAGCAGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.906	93.54	0.532
pilU	Acinetobacter baylyi ADP1	53.803	91.731	0.494
pilU	Vibrio cholerae strain A1552	51.429	90.439	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.628	97.158	0.385
pilT	Pseudomonas stutzeri DSM 10701	42.075	89.664	0.377
pilT	Pseudomonas aeruginosa PAK	41.279	88.889	0.367