Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ONG87_RS01775 Genome accession   NZ_OX352319
Coordinates   391452..393560 (+) Length   702 a.a.
NCBI ID   WP_264382092.1    Uniprot ID   -
Organism   Pseudomonas sp. MM227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386452..398560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONG87_RS01755 (AHFPHNDE_00348) pilM 388601..389665 (+) 1065 WP_122480446.1 pilus assembly protein PilM Machinery gene
  ONG87_RS01760 (AHFPHNDE_00349) - 389666..390235 (+) 570 WP_185794938.1 PilN domain-containing protein -
  ONG87_RS01765 (AHFPHNDE_00350) pilO 390232..390891 (+) 660 WP_122480444.1 type 4a pilus biogenesis protein PilO -
  ONG87_RS01770 (AHFPHNDE_00351) pilP 390888..391415 (+) 528 WP_264382091.1 type 4a pilus biogenesis lipoprotein PilP -
  ONG87_RS01775 (AHFPHNDE_00352) pilQ 391452..393560 (+) 2109 WP_264382092.1 type IV pilus secretin PilQ Machinery gene
  ONG87_RS01780 (AHFPHNDE_00353) aroK 393565..394083 (+) 519 WP_043191342.1 shikimate kinase AroK -
  ONG87_RS01785 (AHFPHNDE_00354) aroB 394164..395267 (+) 1104 WP_264382093.1 3-dehydroquinate synthase -
  ONG87_RS01790 (AHFPHNDE_00355) - 395338..396912 (+) 1575 WP_122480664.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 702 a.a.        Molecular weight: 75548.80 Da        Isoelectric Point: 4.9227

>NTDB_id=1155276 ONG87_RS01775 WP_264382092.1 391452..393560(+) (pilQ) [Pseudomonas sp. MM227]
MNRILSAFGISLGIAMLSPIVMAANLKTLDVASLPGDRVELKLSFDGPVTAPRGYTTEQPARIALDLPGVTSQLGARSRD
LGVGNARSVVVVEATDRTRIIVNLTKLAPYSTRVQGNDLFVLVGEGAAALQGTAIPAPTAARPAPRPAAVPAPAPARAAV
PVGRAIRNVDFQRGELGEGNVIIDLSDPTISPDIQEQGGKILLTFAKTQLPDPLRVRLDVKDFATPVQFVNSSVGATGAT
VTIEPSGSFDYSSYQTENRLTISVRPLTNEDVARRNVERNVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGG
ITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQRQIQELAPLRRELMQVNYAKAADIAKLFQ
SVTRGENVETERGSITVDERTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVGYNKALGVRWGGSTNLRGK
NNWTAYGLDNNGDEAGNTGADVGSNTPFIDLGAADATSGIGLGFVTNNVLLDLELSAMESTGNGEIVSQPKVVTSDKETA
KILKGTEVPYQEASSSGATTVSFKEASLSLEVTPQITPDSRIIMEVKVTKDEPDYLNAVLGVPPISKNEVNAKVLVNDGE
TIVIGGVFSNTQSKTVDKVPFLGDVPYVGRLFRRDVVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2109 bp        

>NTDB_id=1155276 ONG87_RS01775 WP_264382092.1 391452..393560(+) (pilQ) [Pseudomonas sp. MM227]
ATGAACAGGATTCTCTCAGCCTTCGGTATTTCGCTAGGGATAGCGATGCTTTCACCGATCGTCATGGCCGCGAACTTGAA
GACGCTCGACGTTGCTTCCCTGCCCGGTGATCGCGTCGAGCTCAAGCTTTCGTTCGACGGCCCGGTCACGGCGCCACGCG
GCTATACCACCGAGCAACCGGCCCGGATCGCCCTCGACCTGCCTGGCGTGACCAGTCAGCTGGGCGCCCGCAGTCGCGAC
CTGGGCGTGGGCAACGCCCGCAGCGTGGTGGTGGTCGAGGCCACCGACCGCACGCGGATCATCGTCAACCTGACCAAGCT
GGCGCCCTACAGCACCCGTGTGCAGGGCAACGACCTGTTCGTGTTGGTGGGCGAGGGTGCCGCGGCCCTGCAGGGTACGG
CGATACCGGCGCCGACAGCGGCCAGGCCCGCCCCGCGACCCGCAGCCGTGCCTGCGCCTGCGCCTGCGCGCGCGGCGGTG
CCCGTCGGCCGGGCCATTCGCAACGTCGATTTTCAGCGCGGCGAGCTGGGTGAGGGCAATGTCATCATCGACCTGAGCGA
CCCGACCATCAGCCCCGATATCCAGGAGCAGGGCGGCAAGATCCTGCTCACTTTCGCCAAGACCCAGCTGCCCGATCCGT
TGCGCGTGCGTTTGGACGTGAAGGATTTCGCGACCCCGGTGCAGTTCGTCAACTCCTCGGTCGGCGCCACTGGCGCGACC
GTGACCATCGAGCCCAGCGGCAGCTTCGACTACTCTTCGTACCAGACCGAAAACCGCCTGACCATCAGTGTGCGGCCGCT
GACCAACGAGGACGTCGCGCGGCGCAATGTCGAGCGCAATGTCTATACCGGCGAGAAGCTCTCGCTGAACTTCCAGGACA
TCGACGTGCGTTCGGTGCTGCAGCTGATCGCCGATTTTACCAACCTCAACCTGGTGGCCAGTGACACGGTGCAGGGTGGC
ATCACCCTGCGCCTGCAGAACGTTCCCTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAACGCAA
GGTCGGCAACGTGCTGCTGGTGGCCCCGGCCGACGAGATTGCCGCCCGCGAGCGCCAGGAGCTGGAGTCGCAGCGGCAGA
TCCAGGAGCTGGCGCCGCTGCGCCGCGAGTTGATGCAGGTCAACTATGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAG
TCGGTGACCCGTGGCGAGAACGTCGAGACCGAGCGTGGCTCGATCACCGTCGACGAACGCACCAACAACATCATTGCCTA
CCAGACCCAGGATCGCCTCGACGAACTGCGTCGTATCGTTACCCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGG
CGCGCATCGTCGAAGCCAACGTCGGCTACAACAAGGCGCTGGGCGTGCGTTGGGGCGGTTCGACCAACCTGAGGGGCAAG
AACAACTGGACGGCCTACGGCCTGGACAACAATGGCGACGAAGCCGGCAACACCGGCGCCGACGTCGGCAGCAACACGCC
CTTCATCGACCTGGGGGCCGCCGATGCAACCTCCGGCATCGGCCTGGGCTTCGTGACCAACAACGTTCTGCTGGACCTGG
AGCTCAGTGCCATGGAAAGCACCGGCAATGGCGAGATCGTCTCCCAGCCCAAGGTGGTGACCTCGGACAAGGAAACCGCG
AAGATCCTCAAGGGCACCGAGGTACCGTATCAGGAGGCCAGCTCCAGCGGCGCCACCACGGTGTCGTTCAAGGAGGCCTC
GCTGTCACTTGAGGTGACGCCGCAGATCACCCCGGACAGCCGCATCATCATGGAAGTGAAGGTCACCAAGGATGAGCCCG
ACTACCTGAACGCGGTGCTGGGTGTACCGCCGATCAGCAAGAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAG
ACCATCGTCATCGGTGGGGTTTTCTCCAACACGCAAAGTAAAACCGTGGACAAGGTGCCCTTTTTGGGCGACGTGCCGTA
TGTTGGGCGCCTTTTCCGTCGGGATGTCGTGTCGGAAGCCAAATCCGAGCTGCTGGTATTCTTGACACCGCGTATCATGA
ATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(385-449)

Bacterial type II and III secretion system protein (Secretin)

(537-693)

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(310-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.917

100

0.748
  comQ Acinetobacter baylyi ADP1

41.509

100

0.439
  pilQ Acinetobacter baumannii strain A118

38.369

100

0.409
  pilQ Acinetobacter baumannii D1279779

38.369

100

0.409
  pilQ Legionella pneumophila strain Lp02

38.953

98.006

0.382
  pilQ Legionella pneumophila strain ERS1305867

38.953

98.006

0.382

Multiple sequence alignment