Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OGY80_RS08260 Genome accession   NZ_OX336253
Coordinates   1684919..1686598 (-) Length   559 a.a.
NCBI ID   WP_107810558.1    Uniprot ID   -
Organism   Neisseria sp. Marseille-Q5346     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1679919..1691598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OGY80_RS08230 lspA 1680274..1680780 (-) 507 WP_263340404.1 signal peptidase II -
  OGY80_RS08235 glyQ 1680929..1681828 (-) 900 WP_263340407.1 glycine--tRNA ligase subunit alpha -
  OGY80_RS08240 yacG 1681910..1682092 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  OGY80_RS08245 coaE 1682089..1682709 (-) 621 WP_263340410.1 dephospho-CoA kinase -
  OGY80_RS08250 pilD 1682709..1683596 (-) 888 WP_263340414.1 A24 family peptidase Machinery gene
  OGY80_RS08255 pilG 1683598..1684839 (-) 1242 WP_063069313.1 type II secretion system F family protein Machinery gene
  OGY80_RS08260 pilF 1684919..1686598 (-) 1680 WP_107810558.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OGY80_RS08265 carB 1687002..1690217 (-) 3216 WP_049335314.1 carbamoyl-phosphate synthase large subunit -
  OGY80_RS08270 - 1690240..1690800 (-) 561 WP_263340425.1 hypothetical protein -
  OGY80_RS08275 - 1691104..1691361 (-) 258 WP_016687622.1 SemiSWEET family transporter -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62236.46 Da        Isoelectric Point: 6.0382

>NTDB_id=1154764 OGY80_RS08260 WP_107810558.1 1684919..1686598(-) (pilF) [Neisseria sp. Marseille-Q5346]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1154764 OGY80_RS08260 WP_107810558.1 1684919..1686598(-) (pilF) [Neisseria sp. Marseille-Q5346]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCAGGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGGGAGTTAGTTGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCACTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTTCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTAGTGGTTCCCGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGCCAACGCTCGACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACGCAGCAATCTCAATCG
CTTTATATCGATAACGAAGAAGCTGAAGACGGCCCTATCCCCCGCTTCATCCATAAAACCTTATCCGATGCCCTGAATGC
GGGCGCATCAGACATCCACTTTGAATTCTACGAGCAAATGGCACGCGTCCGTTTCCGCGTGGACGGACAATTGCGCGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTAGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGCGTCAGCACGTTGCC
GACATTGTTCGGCGAAAAAGTGGTAATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAAGCTATCAACCGCCCTTACGGCATGGTGTTGGTTACCGGCCCGACAGGTTCG
GGCAAAACCGTTTCGCTCTATACCTGCCTGAGTATTTTGAACACGGAAGATGTGAACATTTCAACGGCAGAAGATCCGGC
CGAGATTAACTTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGTCTGACGTTTGCTGCCGCACTCCGATCCT
TCTTACGCCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCTACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
AGCGCCTTTCAACATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTTGAACGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAGCTC
TACCGCCCGGTTGGCTGCGACAGTTGTCGCGGCAAAGGCTTTAAAGGCCGCGTCGGCATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAAGAAGGCATGGTAG
ACTTGCGTCGCGCCGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTGACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.46

100

0.775
  pilB Acinetobacter baylyi ADP1

51.056

100

0.519
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.602

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.547

96.064

0.37

Multiple sequence alignment