Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C6H39_RS25950 Genome accession   NZ_LT963409
Coordinates   5467414..5469519 (-) Length   701 a.a.
NCBI ID   WP_060402733.1    Uniprot ID   A0A2K4X1K8
Organism   Pseudomonas syringae isolate CFBP3840     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5462414..5474519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H39_RS25935 (CFBP3840_05015) - 5464048..5465661 (-) 1614 WP_060402735.1 SPOR domain-containing protein -
  C6H39_RS25940 (CFBP3840_05016) aroB 5465672..5466775 (-) 1104 WP_060402734.1 3-dehydroquinate synthase -
  C6H39_RS25945 (CFBP3840_05017) aroK 5466891..5467409 (-) 519 WP_002551657.1 shikimate kinase AroK -
  C6H39_RS25950 (CFBP3840_05018) pilQ 5467414..5469519 (-) 2106 WP_060402733.1 type IV pilus secretin PilQ Machinery gene
  C6H39_RS25955 (CFBP3840_05019) pilP 5469567..5470094 (-) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  C6H39_RS25960 (CFBP3840_05020) pilO 5470091..5470714 (-) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  C6H39_RS25965 (CFBP3840_05021) - 5470711..5471310 (-) 600 WP_060402732.1 PilN domain-containing protein -
  C6H39_RS25970 (CFBP3840_05022) pilM 5471310..5472374 (-) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75513.88 Da        Isoelectric Point: 5.0976

>NTDB_id=1148887 C6H39_RS25950 WP_060402733.1 5467414..5469519(-) (pilQ) [Pseudomonas syringae isolate CFBP3840]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDSPVPAPRGYTTAQPARIALDLPGVTSQLATKSRE
LGNGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLYVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVIIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VASAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNG
KWTTYGLDNNGDEASNTGSNVTSNVPFVDMGASGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNALLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=1148887 C6H39_RS25950 WP_060402733.1 5467414..5469519(-) (pilQ) [Pseudomonas syringae isolate CFBP3840]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTAAA
GACTCTGGATGTCGCCGCCCTGCCGGGTGATCGCATCGAGCTCAAGCTGGCATTCGACTCGCCGGTGCCTGCGCCGCGCG
GTTACACCACGGCGCAACCTGCGCGTATTGCGCTGGACCTGCCGGGCGTGACCAGTCAGTTGGCGACCAAAAGCCGTGAA
CTCGGTAACGGTAACGCACGCAGCGTGGTCGTTGTACAGGCTCAGGACCGCACGCGGGTAATCATCAACCTGACCACGCT
TTCGCCCTACAGCTCCCGCGTCGACGGCAACAACCTTTACGTGGTTATCGGTCAGGGCGCAGCCGCTGCGCAGGCTTCCC
AGCCAACCACCGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCACCCACTCCGGCATCTGCCAGACCGTTTATCCCCGCG
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTGATCATAGATTTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTCGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTGCGGCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTTGTCAGTGCGTCGGCTACGGGCGATAAGGCCAGTATC
CTGATCGAACCGTCGGGTGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGTGTGCGGCCGTTGAC
CAATGAGGATCTGGACCGCCGCGCCGCTGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTACGCTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTAGCCAGCGACACGGTTCAGGGCGGTATT
ACCCTGCGTCTGCAGAACGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCCGCCGATGAAATTGCTGCCCGCGAGCGTCAGGAGCTTGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAATTGTTGCAGGTCAATTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCG
GTGGCCAGCGCCGAGTCCAAGACCGACGAGCGTGGCTCGATCACTGTGGACGACCGGACCAACAACATCATTGCCTATCA
AACCCAGGACCGGCTCGACGAACTGCGTCGCATCGTCTCGCAACTGGACATTCCGGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTGGGTGTGCGCTGGGGCGGCTCGACCAACACCAGCGGCAATGGA
AAATGGACAACCTATGGCCTTGATAATAATGGCGATGAGGCAAGTAACACTGGCAGCAACGTGACCTCCAACGTGCCGTT
TGTCGATATGGGCGCATCTGGCGCAACCTCCGGCATTGGCCTGGGCTTCGTCACCAACAATACGTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGTAACGGTGAAATCGTCTCCCAACCCAAGGTAGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACCGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCAACCACTGTGAGCTTCAAGGAGGCCTCGCT
GTCGCTTGAGGTAACGCCACAGATCACGCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGTTGCTTGGTGTACCACCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTCATTGGTGGTGTGTTCTCAAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Secretin and TonB N terminus short domain (STN)

(309-355)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K4X1K8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.646

100

0.739
  comQ Acinetobacter baylyi ADP1

40.463

100

0.424
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.405
  pilQ Legionella pneumophila strain Lp02

38.495

98.573

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.406

98.431

0.378

Multiple sequence alignment