Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6H37_RS04960 Genome accession   NZ_LT963408
Coordinates   957902..959596 (-) Length   564 a.a.
NCBI ID   WP_104698279.1    Uniprot ID   -
Organism   Pseudomonas syringae group genomosp. 3 isolate CFBP6411     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 952902..964596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H37_RS04925 (CFBP6411_00885) - 953034..953567 (-) 534 WP_005614784.1 MOSC domain-containing protein -
  C6H37_RS04930 (CFBP6411_00886) - 953564..954193 (-) 630 WP_005736090.1 DUF1780 domain-containing protein -
  C6H37_RS04935 - 954390..954716 (+) 327 WP_005736089.1 hypothetical protein -
  C6H37_RS04940 (CFBP6411_00887) yacG 954982..955191 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  C6H37_RS04945 (CFBP6411_00888) coaE 955188..955811 (-) 624 WP_005736088.1 dephospho-CoA kinase -
  C6H37_RS04950 (CFBP6411_00889) pilD 955808..956680 (-) 873 WP_005736087.1 prepilin peptidase Machinery gene
  C6H37_RS04955 (CFBP6411_00890) pilC 956682..957899 (-) 1218 WP_005736085.1 type II secretion system F family protein Machinery gene
  C6H37_RS04960 (CFBP6411_00891) pilB 957902..959596 (-) 1695 WP_104698279.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C6H37_RS04965 (CFBP6411_00892) pilA2 959823..960251 (+) 429 WP_005736082.1 pilin Machinery gene
  C6H37_RS04970 (CFBP6411_00893) - 960414..960779 (-) 366 WP_005736080.1 winged helix-turn-helix transcriptional regulator -
  C6H37_RS04975 (CFBP6411_00894) gstA 960937..961557 (+) 621 WP_058975496.1 glutathione transferase GstA -
  C6H37_RS04980 (CFBP6411_00895) - 961656..962201 (-) 546 WP_003383227.1 DUF1523 family protein -
  C6H37_RS04985 (CFBP6411_00896) - 962322..963320 (+) 999 WP_005736077.1 ATP-grasp domain-containing protein -
  C6H37_RS04990 (CFBP6411_00897) - 963375..964523 (+) 1149 WP_005736076.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62489.78 Da        Isoelectric Point: 6.3854

>NTDB_id=1148842 C6H37_RS04960 WP_104698279.1 957902..959596(-) (pilB) [Pseudomonas syringae group genomosp. 3 isolate CFBP6411]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLVSLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLATQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1148842 C6H37_RS04960 WP_104698279.1 957902..959596(-) (pilB) [Pseudomonas syringae group genomosp. 3 isolate CFBP6411]
ATGACTGATGCTGTCCTTACTGGCTTGGCCAAACAATTGGTCCTCGCCGAGCTCCTTACTGAACCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGTCGCGACAAGATCTCGTTGGTGAGCTATCTGGTTCAGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCCGACCAGTTTGGCGTGCCGTTCATGGACCTCGTCAGCCTGGACAAGGAAAGCCAGCCCAAAGGCCTG
GTGAGTGAGAAGCTGGTTCGCCAGCATCACGCCCTGCCGCTGTGGCGACGCGGTAACAAGCTCTTTATCGGGATTTCCGA
CCCGACCAATCATCAGGCTGTGACCGATATTCAGTTCAGTACTGGCCTGAACACCGAAGCGATTCTGGTCGAAGATGACA
AGCTGACCACTGCGATAGATCGATTCTTTGACAGTGACAGCGGTCTGGGTAACTTGGAAGATGTCGATCTGGGCCTGGAT
ATCGAACCTGCTGATGGCAAGGAAACCTCCCTCGCGACGCAGAATGACGCCGACGACGCCCCCGTGGTCCGTTTCGTCAA
TAAAATGCTGCTGGATGCAATCCGGCTAGGATCATCCGATTTACATTTCGAACCCTACGAAAAGATCTTTCGCGTGCGCC
TACGCACCGACGGCATTTTGCATGAAGTTGCCAGGCCGCCGATTCACCTGGCAAACCGTATTGCAGCGCGCTTGAAAGTC
ATGGCAAGCCTCGATATCTCGGAGCGGCGCAAACCGCAGGACGGGCGGGTCAAGTTGCGGGTGTCCAAAACCAAGGCGAT
TGATTTTCGTATGAATACCTTGCCCACACTGTGGGGCGAGAAGATCGTGATGCGGATCCTGGACCCGACCAGCGCGCAGA
TGGGCATTGATGCTCTAGGCTACGAGCCAGAACAGAAAGCACTGTATCTGGAGGCGCTAAAACAGCCCCAGGGCATGATT
CTTGTGACTGGCCCCACGGGTTCCGGCAAGACCGTTTCCCTGTATACCGGTCTGAATATCCTTAATACGGTGGACATCAA
CATTTCCACAGCTGAAGACCCCGTGGAGATCAATCTGGAAGGTATTAATCAGGTCAACGTCAACCCGCGTCAGGGGCTGG
ACTTTTCGCAGGCATTGCGCGCCTTCTTGCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATACGAGACTTGGAAACC
GCCGAAATCGCCATAAAGGCATCACAAACCGGCCACATGGTGCTGTCCACCCTGCACACCAACAGCGCTGCTGAAACCTT
GACGCGACTGCATCATATGGGCGTGGCCGCATTTAATATCGCCACTGCAATCAACCTGATTATTGCGCAACGGCTCGCGC
GCAAGCTGTGCAGCCATTGCAAGAAAGAACTCGATATTCCTCGCGAGACACTGATTCGGGAAGGTTTTCCAGAAGCGAAG
ATTGGCACTTTCAAGATCTATGGTCCGGTGGGCTGCGAACATTGCAATGGTGGCTATAGAGGCAGGGTCGGTATCTACGA
AGTCGTCAAGAAAACAGCAGAGCTGGAGCGCATCATCATGGAAGAGGGTAACTCACTGGAGATTTCCAGGCAGATGCGCA
AGGACGGCTTCAACGACCTGCGAACCTCAGGCCTGTCAAAAGCCATGCAGGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.672

100

0.58
  pilB Acinetobacter baylyi ADP1

56.537

100

0.567
  pilB Legionella pneumophila strain ERS1305867

55.575

100

0.557
  pilB Vibrio cholerae strain A1552

50.89

99.645

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.626

99.113

0.502
  pilB Vibrio campbellii strain DS40M4

49.47

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.128

99.468

0.479
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.973

93.262

0.363

Multiple sequence alignment