Detailed information NTG1148549

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PL963_RS15150
• Genome accession: NZ_LT962480
• Coordinates: 3417105..3418274 (-)
• Length: 389 a.a.
• NCBI ID: WP_003400837.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae isolate CFBP4215
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3412105..3423274

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PL963_RS15135 (CFBP4215_02916)	-	3413389..3414453 (+)	1065	WP_003400842.1	microcin C ABC transporter permease YejB	-
PL963_RS15140 (CFBP4215_02917)	-	3414453..3415475 (+)	1023	WP_003400841.1	ABC transporter permease	-
PL963_RS15145 (CFBP4215_02918)	-	3415477..3417054 (+)	1578	WP_058822110.1	ABC transporter ATP-binding protein	-
PL963_RS15150 (CFBP4215_02919)	pilU	3417105..3418274 (-)	1170	WP_003400837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PL963_RS15155 (CFBP4215_02920)	-	3418398..3418673 (+)	276	WP_104684293.1	peptidylprolyl isomerase	-
PL963_RS15160 (CFBP4215_02921)	pgm	3418748..3420394 (-)	1647	WP_011268077.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PL963_RS15165 (CFBP4215_02922)	-	3420558..3422885 (-)	2328	WP_032657338.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42577.65 Da
• Isoelectric Point: 6.0641

>NTDB_id=1148549 PL963_RS15150 WP_003400837.1 3417105..3418274(-) (pilU) [Pseudomonas syringae pv. syringae isolate CFBP4215]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1148549 PL963_RS15150 WP_003400837.1 3417105..3418274(-) (pilU) [Pseudomonas syringae pv. syringae isolate CFBP4215]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TGATGGACGAAGAACAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTTATGCAGCGCAACGAAGTGTCCATCGTCGCGCGGAATATCAAACTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACGGGTT
CGGGCAAGTCGACCTCATTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGCGGCCATATCATCACCATTGAGGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCATTGAAAAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
ACTGGTCAGAACACCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCGGCGCTG
TTCAATCTGGCCGTCGAAGGGGCGATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAGAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGAGCCTCACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.287	93.059	0.542
pilU	Vibrio cholerae strain A1552	51.105	93.059	0.476
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362