Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   LSAF01_RS06510 Genome accession   NZ_LT960777
Coordinates   1299339..1299638 (-) Length   99 a.a.
NCBI ID   WP_011374999.1    Uniprot ID   Q38W25
Organism   Latilactobacillus sakei strain FLEC01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294339..1304638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSAF01_RS06475 (LSAF01_1312) - 1294970..1295428 (-) 459 WP_011374992.1 hypothetical protein -
  LSAF01_RS06480 (LSAF01_1313) - 1295483..1296679 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  LSAF01_RS06485 (LSAF01_1314) - 1296701..1297711 (-) 1011 WP_099947695.1 class I SAM-dependent methyltransferase -
  LSAF01_RS06490 (LSAF01_1315) - 1297835..1298173 (-) 339 WP_035145011.1 hypothetical protein -
  LSAF01_RS06495 (LSAF01_1316) comGF 1298136..1298648 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  LSAF01_RS06500 (LSAF01_1317) comGE 1298623..1298928 (-) 306 WP_016265375.1 hypothetical protein Machinery gene
  LSAF01_RS06505 (LSAF01_1318) comGD 1298915..1299367 (-) 453 WP_035145016.1 competence type IV pilus minor pilin ComGD Machinery gene
  LSAF01_RS06510 (LSAF01_1319) comGC 1299339..1299638 (-) 300 WP_011374999.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LSAF01_RS06515 (LSAF01_1320) comGB 1299635..1300642 (-) 1008 WP_011375000.1 type II secretion system F family protein Machinery gene
  LSAF01_RS06520 (LSAF01_1321) comGA 1300635..1301525 (-) 891 WP_016265378.1 competence type IV pilus ATPase ComGA Machinery gene
  LSAF01_RS06525 (LSAF01_1322) - 1301640..1302371 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LSAF01_RS06530 (LSAF01_1323) - 1302462..1302962 (-) 501 WP_099947696.1 VanZ family protein -
  LSAF01_RS06535 (LSAF01_1324) - 1303059..1303433 (+) 375 WP_011375004.1 hypothetical protein -

Sequence

Protein


Download         Length: 99 a.a.        Molecular weight: 10994.02 Da        Isoelectric Point: 10.2663

>NTDB_id=1148364 LSAF01_RS06510 WP_011374999.1 1299339..1299638(-) (comGC) [Latilactobacillus sakei strain FLEC01]
MKKKRKAFTLIEMVVVLAIIALLILLVAPNLGAQKQRAEKKTDQALVTTLQTQVELASDEIGHQIKSLDELTDKYISKDQ
LKHAKEKGITIEGGTVKQK

Nucleotide


Download         Length: 300 bp        

>NTDB_id=1148364 LSAF01_RS06510 WP_011374999.1 1299339..1299638(-) (comGC) [Latilactobacillus sakei strain FLEC01]
ATGAAGAAAAAAAGAAAAGCTTTTACACTAATTGAAATGGTAGTTGTTCTTGCCATTATTGCGTTACTAATCTTATTAGT
TGCACCAAACCTAGGTGCTCAAAAACAGCGCGCGGAGAAGAAAACGGATCAAGCTTTGGTGACGACTTTGCAAACGCAAG
TTGAATTAGCTAGCGACGAGATTGGTCATCAAATTAAGAGTCTAGACGAATTAACGGATAAGTATATTTCAAAAGACCAA
TTAAAGCACGCAAAGGAGAAGGGGATTACAATTGAAGGCGGCACGGTTAAGCAAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q38W25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Latilactobacillus sakei subsp. sakei 23K

100

100

1
  comGC/cglC Streptococcus pneumoniae TIGR4

45.349

86.869

0.394
  comGC/cglC Streptococcus pneumoniae Rx1

45.349

86.869

0.394
  comGC/cglC Streptococcus pneumoniae D39

45.349

86.869

0.394
  comGC/cglC Streptococcus pneumoniae R6

45.349

86.869

0.394
  comGC/cglC Streptococcus mitis NCTC 12261

44.186

86.869

0.384

Multiple sequence alignment